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Lividans. Using pIJ903 bearing tsr gene as a vector, the two furthermost fragments of the cluster, 4. 0kb and 1. 5kb in size respectively, were inserted into it. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. OriT from E coli plasmid RK2 was also incoporated into the vector, therefore, pIJ903 derivatives can be mobilized from E.

在具有硫链丝菌素抗性基因的pIJ903的单—BamHI位点,同向插入了FR-008生物合成基因簇两个最远端的4.0kb和1.5kb片段,并在二者之间插入了可在链霉菌FR-008和变铅青链霉菌中表达的阿泊拉霉素抗性基因,同时也插入了有助于将质粒引入链霉菌的oriT片段,从而构建出了置换整个基因簇的基因置换质粒pHZ691。

In news reports, to call a woman "grandmotherly" is for "kindly, frail, harmless, keeper of the family antimacassars, and operationally past tense."

在新闻报导中,称一位妇女为"慈祥的"就是一种公式化的表达即"和善的,脆弱的,好心的,可胜任的家庭监护人。"这已是过去式了。

The language of uncertainty has three main categories:(1)words such as probably, possibly, surely, which denote a single subjective probability and are potentially quantifiable;(2)words like many, of-ten, goon, which are also quantifiable but denote not so much a condition of uncertainty as a quantity imprecisely known;(3)words like fat, rich, drunk, which can not be reduced to any accepted number because they are given different values by different people.

模糊语言可分为三类:1。表示单一主观意义上可能性的,可能定量的词语,诸如"很可能"、"可能"、"肯定"等词。2。诸如"许多"、"时常"、"不久"等词语,这类词语也可以定量,但所表达的意义与其说是某种不能肯定的状况,不如说是一个知之不确的数量。3。诸如"胖"、"富"、"醉"这类词语,不能精确到人们都认同的数量,只因不同的人有不同的评判标准。

Objective Consequently, by constructing the pcDNA3-HA-hdacl, a HDAC1 eukaryotic expression vector, we were to study the interaction between HDAC1 and ERa and ERB in vitro and the effects of HDAC1 on the ERa and ERB at the protein level. So we can partly reveal the multiplication, differentiation and cancerization of lacteal gland cells.

目的 本文意在通过构建可在真核细胞中表达的重组质粒pcDNA3-HA-hdacl,研究组蛋白去乙酰化酶1(HDAC1)和雌激素受体ERα和ERβ在体外的相互结合作用,以及HDAC1对ERα和ERβ蛋白水平的影响规律,从而在一定角度揭示乳腺细胞的增殖、分化和癌变的过程,为更好地寻找治疗癌症的药物靶标打下基础。

So packing oneself in his single world can help nothing to add his reputation, what he strives to do is the reverse-anatomize oneself in front of the public stares and acclaim for his names being praised or criticized in the SUN -then can he make himself known out of the nonplus of being forgotten and lose his prestige in public.

这里我们已经知道作这篇文章要从"公众人物"自身出发,以他们的角度来看待自己隐私是否该暴光的问题,这样我们就找到中心回应点是"should expect",对象是"public figures",而例证可以充分利用话题的友好提示:列举的三种人,选择熟悉易于表达的来充实论证,免了自己的劳神工夫,这种资源一定要善加利用,一方面可充实论据,另一方面,作到了兼顾其他,全面回应的原则。

The uniqueness of solution of field point, inside a convex region due to singular source with kernel function decreasing with distance increasing, outside-region-distribution such that the boundary condition expressed by the response of the source is satisfied, is proved by using the condition of kernel function decreasing with distance increasing and an integral inequality.

应用奇源解的核函数沿距离递减的条件和积分不等式,可证这类奇源在凸域外分布只要满足以它产生的响应来表达的边界条件时,所引起相应的域内场点解是唯一的。

At Prime Minister's Question Time, he said there was entirely understandable fearin the community and said all MPs would want to send their sympathy to the people of Suffolk and the relatives of the victims.

在首相的提问时间,他说整个社区都笼罩在"彻底的可理解的恐惧"之中,他还说所有的议员们都想表达他们对萨福克郡的居民及受害者的亲属的同情。

The results demonstrate that a greatly increased bioavailability of As under the flooded conditions is the main reason for an enhanced As accumulation by flooded rice, and growing rice aerobically can dramatically decrease the As transfer from soil to grain.

这两种硅运输蛋白都在根部表达,其中一种称为Lsi1的蛋白是亚砷酸盐进入水稻的门户,而另一种称为Lsi2的蛋白控制着从根部到茎再到谷粒的流动。这组研究者还发现硅酸—含有硅、氢和氧—会干扰亚砷酸盐的摄取。他们提出,增加土壤中可利用的硅,可能会减少从土壤和灌溉用水到水稻的砷的运输。(生物谷Bioon.com

You can do anything but lay off of my blue suede shoes.

话说我英语其实不大好,可我看那歌词真的感觉怪怪的,我的理解是像上面那样的,译得不好大家也别笑阿因为我就是直译的,可能我不了解他真正想表达的也不一定

On the other hand, Rothko's works are all very large.

克利的作品尺寸都不大,却表达了深邃的意境,画面上可看的东西很多,还有些幽默感,我喜欢他的线条和对色彩的处理。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。