可移植的

For those fungal corneal ulcers that are poorly responsive to anti-fungus eye drops, it is recommended to perform lamellar keratoplasty if the ulcer is located in the anterior 1/2 stroma, the key points of surgery are to remove the affected tissue completely and cauterize the recipient bed with 5% iodine.

采用无水甘油或无水氯化钙保存的角膜片可对药物治疗效果不显或对现有药物耐药的真菌性角膜溃疡者行治疗性角膜移植术，可及时去除病灶、控制炎症、保存眼球，为再次增视性角膜移植术创造条件。

In 8 cm grafted group , all survival dogs(n=5) were held upright with the affected hindlimb extended so that the body's weight was supported by the distal metatarsus and toes. In 10 cm grafted group, animals were failed to held upright with the affected hindlimb.

体内移植实验结果显示8 cm去细胞异体神经移植组的犬在术后12个月踝关节可直立行走，而10 cm去细胞异体神经移植组犬12个月时踝关节不能直立行走。

Prussian blue staining revealed more than 98% of BM-MNCs were labeled by Feridex and BM-MNCs appeared to be unaffected by magnetically labeling. Three dogs injected Feridex- BM-MNCs were visualized at one week by MRI, but none of unlabeled BM-MNCs were seen. All lesions that were detected within one week could not be visualized over four week. The dot injected Feridex-cells which fixated by 4 % methanal also was seen at one week by MRI, but the signal disappeared at two week.

普鲁士蓝染色显示体外Feridex标记BM-MNCs成功率达98%以上，而且细胞活性不受影响；其中3只实验犬标记细胞移植区1周内可用1.5T磁共振仪3DSSFP序列在体扫描到T2低信号影像，4周时该低信号消失；同样条件下经甲醛固定的标记细胞在1周时也可检测到T2低信号，但2周时该低信号消失；未标记细胞移植区没有检测到低信号。

We examined 8 cases after costal cartilage autograft in clinical observation through optics microscope, verified that the framework keeps the nature of costal cartilage and pointed out the importance of prevention of infection. Ear reconstruction can be completed in preschool age because we observed that the reconstructed ear in preschool age could grow with the normal ear in pace (7 cases). With perichondrium costal cartilage autograft could live and grow better.

临床观察8例肋软骨移植后的组织学检查，证实了耳支架成活后保持了肋软骨的生物学特性；强调了预防感染对移植耳支架的转归及治疗效果的重要性；观察到7例学龄前儿童自体肋软骨移植后再造耳廓能随年龄同步按比例生长，表明耳廓再造可在学龄前6岁时完成。

The induction of self heat shock protein immune reactivity can attenuate inflammatory reaction and delay transplant immunity.On the other hand,HSPs can also exacerbate transplant immunity by activating innate immunity system and adaptive immunity system.

HSPs适当的表达可能会减轻炎性反应，从而抑制移植排斥；另一方面，HSPs也可激活天然免疫系统和获得性免疫系统，加重移植排斥反应。

The progress of CAN can be torpidity by SRL ,and the survival of allografts were prolonged significantly.

SRL可降低移植肾组织TFG-β1和VEGF表达，减缓慢性移植肾肾病的进展，延长移植肾存活时间。

Conclusion］Good results can be obtained in hips with acetabular protrusion after reconstructed with autogeneous or allogeneous bone grafting and total hip arthroplasty.

结论］应用自体股骨头骨或异体颗粒骨移植填充髋臼连系多孔生物型髋臼假体，治疗Otto's病，可获得较好的疗效，移植骨可与宿主骨很好地连系在一路。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The quantity of CD3+ cells in the EdNSC, AdNSC of cloning goattransplantation group and control group had no statistics difference(P＞0.05) at acute and chronic period.Overall, in our study, We got the conclusions as follow: 1,The EdNSC, AdNSCof cloning goat and the AdNSC of the non-cloning goat were successful obtained;EdNSC growed faster than AdNSC in the same cell culture method. 2,The braincontusion and laceration model of cloning goats were successful established by freefalling of 50 gram of steel ball accelerate strike on the goat brain from 100cm height.3, EdNSC and AdNSC transplantation both had obvious therapeutic effect on braincontusion and laceration of cloning goats; The therapeutic effect of EdNSCtransplantation was better. 4, Allograft of stem cells has acute and chronicimmunological rejection and autografting of stem cells has no acute and chronicimmunological rejection.

在相同的条件下，EdNSC体外培养速度比成AdNSC快。2对羊脑上薛氏沟周围运动皮层区使用50g×100 cm力度作自由落体加速撞击，可产生观察指标明确且存活率高的羊脑挫裂伤模型，有望广泛用于神经干细胞移植及药物等治疗的实验研究。3胚胎神经干细胞及成体神经干细胞移植治疗脑挫裂伤后均有较明显的治疗效果；二者相比较而言胚胎神经干细胞稍好于成体神经干细胞。4中枢神经系统异体神经干细胞移植存在着明显的免疫排斥反应，而自体神经干细胞移植基本无免疫排斥反应

Methods : The defect of bone, skin and vessel in the bone and joint of limbs was treated by microsurgical method and the bone and joint injury was repaired by the external fixation supporter of odjustment cross joint with three Kirschner wires, keeping the free motion of every joint.

在四肢关节部位有骨缺损或骨折伴有血管损伤、皮肤缺损等病例，应用带血管的骨关节块移植、游离血管移植或各种皮瓣移植等显微外科方法修复后的同时，用三针可调外固定器跨关节外固定治疗骨关节损伤，保持各关节的自由活动。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！