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With the rapid development of computer ,micro-electronics and network, embedded system has been widely applied in the national defence, industry, medical treatment,etc.lt is very meaningful and important to develop a general platform, which is an integrated production of embedded control, signal processing and network communication, and provide a programmable hardware interface and a friendly interface, which can improve networking flexibility, system reliability and developability.

随着计算机技术、微电子技术和网络技术的飞速发展,嵌入式系统在国防、工业、医疗等诸多领域的应用越来越广泛。研制一个集嵌入式控制、信息处理和网络通信于一体,且提供可编程的硬件接口和友好的软件开发界面的通用平台,对于提高分布式测控系统的组网灵活性、系统可靠性和可开发性都具有重要的意义。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7(MDA-7)的克隆及功能研究:利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列,经测序鉴定序列与文献报道一致后,与真核表达载体pcDNA3.0连接,构建pcDNA3-MDA-7表达载体,瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA,RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达;转染48h后测定细胞增殖和活力,MDA-7可选择性抑制肿瘤细胞的增殖,对A549、Hela和HepG2细胞的抑制率分别为25%、20%和19%,但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后,以G418筛选2周后计数单层细胞集落形成数,计数仅为转染pcDNA3.0空载体的细胞的30%左右。

According to 'cluster analysis', testers can be divided into 4 groups:"Moralist","Moderationist","The self serving","The peer-first".

受测者依集群分析,可分为4群︰「卫道人士」、「中庸之道」、「独善其身」、「同侪至上」。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?