可测的

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In our system, we were unable to detect the cleavage product of overexpressed slit2. Nevertheless, it is interesting to investigate whether the cleavage of slit2 is important for its responsible function in CL1-5 or not. Our result showed that a non-cleavable slit2 showed similar growth inhibitory ability as the cleavable slit2. Therefore, the growth inhibitory ability of slit2 does not require cleavage at the reported cleavage site.

结果显示，在CL1-5细胞中，我们没有侦测到Slit2被切割的情形，并且当不能被切割的Slit2蛋白表现在CL1-5时，其抑制生长的能力与可被切割的Slit2相同，因此这个报导中的切割点与Slit2抑制生长的功能无关，而单独过度表现Slit2-C terminal蛋白片段就具有抑制细胞生长的能力。

Regardless to the research development or industrial application, nano metal powders are extensively discussed. Within these powders, the flammable and explosible nano aluminum, titanium and iron powders produce a great amount production and apply widely. Meanwhile, the surface/volume ratio of powders is increased significantly under nano scale, in which to enhance the ignition opportunity. Thus, the dust explosion hazard risk of nano powders could remarkable rise. In addition, the comparison of explosion phenomenon, test methods and quantification parameters between nano and micro scale is necessary to deeply investigate further. So that this project operates the explosion characteristic experiments to measure and determine such the maximum explosion pressure, minimum explosion concentration and explosion characteristic Kst value on above mentioned three nano metal powders. Expectantly, to provide the appropriate recommendations of safety prevention for nano dust explosion based on the experimental results.

奈米金属粉末无论是研究发展或是工业界应用，目前皆是广泛讨论研究之材料，其中，奈米铝粉、奈米钛粉以及奈米铁粉等可燃、爆性粉末，均有高额产量及大量应用领域，与此同时，在粉末粒径进入奈米尺度的情况下，粉尘比表面积大幅提升，增加此三种粉尘可被点燃的机会，因此，潜在的粉尘爆炸危害风险将广幅增加，另外，微米尺度粉尘爆炸的现象、测试方法以及量化参数是否具有相同的实验尺度基点，也需要深入探讨，故本次计画针对上述三种奈米金属粉末，进行三种不同粒径的爆炸特性参数实验，如最大爆炸压力、最低爆炸浓度以及爆炸特性参数Kst值之量测与计算，期能量化其粉尘爆炸特性，并据此提出适当的安全防护建议。

Read and write of flash disk as USB host.According to the request of this subject, the system hardware and software for the slave device, and the communication interface to the master PC are developed. Detailed works are as follows: in order to implement the measurement principle in the system, flow-liquid levelmethod is used;velocity of flow is measured through rotor Kinemometer;water level is surveyedvia RS485 interface and pressure sensor;the C8051F060 chip which is in high performance andintegration, produced by Silicon Laboratories Company as master chip is used to capture and measure the width of impulses, so singals of flow velocity are collected and disposed;the 4×4 matrix keyboard and LCD are used in the part of man-machine interface which are easy to input, legible to read, various to display, comfy to be accepted and plain to show measure and inquiry results;FRAM memory FM3164 is used to carry out the non-volatile memory of data and accurate real-time clock.;SL811HST chip is used as USB host which can read and write flash disk,thereby a kind of adscititious mass storage is accomplished .

根据研制任务的要求，课题期间主要完成了下位机系统硬件和软件，设计了与上位机的通讯界面，所做的具体工作包括：测量原理在系统中的实现，采用了流速—水位计算法，通过旋桨式流速仪来测量流速，通过RS485接口与压力变送器通讯测量水位；主控制芯片采用了Silicon Laboratories公司的高性能、高集成度C8051F060芯片，利用其捕捉测脉宽的特性，实现了流速信号的采集和处理；人机接口部分采用了4×4矩阵键盘和LCD显示，输入方便，读数清晰，显示多样化，信息量大，易于被用户接受，更直观地显示测量及查询结果；通过采用铁电存储器FM3164实现了数据的非易失性存储和精确的实时时钟；通过采用SL811HST芯片，实现了USB主机的功能，可读写U盘，从而实现了单片机的外挂式海量存储。

Results In vitro recovery of the probe was mainly influenced by the perfusion rate. When the perfusion rate was set at 1.0μL/min, in vitro and in vivo recoveries of the probe were 50.16±2.34% and 14.096±0.58%, respectively. The enhancement indexes for 3%, 5% mentha oil were 3.8 and 17.6, respectively. The enhancement indexes were 9.2 and 24.8, for 3% and 5% of Azone, respectively. Conclusion The steady sate flux of meloxicam in rats is significantly improved by enhancer mentha oil and azone.

结果： 在实验条件下微渗析探针的体外回收率主要受灌注液流速的影响，为（50.16±2.34）%；反向渗析法测得探针的在体回收率为（14.096±0.58）%；MO的体积分数为3%和5%时，增渗倍数分别为3.8，17.6；azone的体积分数为3%和5%，增渗倍数分别为9.2，24.8；结论：不同体积分数的MO和azone均可显著增加美洛昔康经皮渗透稳态流量，azone的体积分数为5%时，大鼠角质层的屏障作用可能受到了较大程度的破坏。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

Results indicate that the number of bacteria grown in media LB, CSEA and WSA was 14.84×10^7, 10.27×10^7 and 6.91×10^7 CFU per gram dry soil, respectively, after 192 h of incubation. The diversity index of soil bacteria was the highest in WSA, while the lowest in LB. Certain similarity was discovered in bacteria community between the three media, i. e. the Jaccard index was 57.69% between LB and CSEA, 53.13% between LB and WSA and 66.67% between CSEA and WSA. Phylogenetic analysis suggests that the dominant bacterial groups in the soil belong to β-Proteobacteria,γ-Proteobacteria and Actinobacteria. The sequence of 16S rDNA of some OTUs was found to have high homology with Burkholderiaceae bacterium, Rhodococcus and Mycobacterium, so it is presumed that the cells of these bacteria can secrete Resuscitation-Promoting Factors, which may effectively improve culturability of soil microorganisms.

结果表明，LB、CSEA、WSA培养基192h后每g干土获得的细菌数量分别为14.84×10^7、10.27×10^7和6.91×10^7CFU，但微生物多样性指数以WSA为最高，LB多样性指数最低；三种培养基培养的细菌菌群有一定的相似性，LB和CSEA培养基间的Jaccard指数为57.69%，LB和WSA培养基之间为53.13%，而CSEA和WSA培养基的相似性指数达66.67%；16SrDNA测序结果表明，所获得的土壤细菌优势种群在分类方面主要属于γ-和β-变形杆菌以及放线菌亚门，其中某些OTUs中的16SrDNA序列与Burkholderiaceae bacterium、Rhodococcus和Mycobacterium属具有较高的同源性，推测其细胞能够分泌复苏促进因子，有效地提高土壤细菌的可培养性。

Analysis of nutritional components in pear fruits of different varieties showed that fructose, glucose and sucrose were predominant sugar in pear fruits, the content of which ranged from 3.40% to 9.24%, 0.21% to 2.72%, 0.10% to 1.69%, respectively; Content of titrable acid was the highest in Bayuehong Pear(0.286%) and lowest in Atago(0.078%); Content of Vitamin C was the highest in Beurre Bosc (9.50 mg/100g) and lowest in Lubaoshi Pear(1.89mg/100g); Content of protein ranged from 127 to 428 mg/100g, content of free amino acid ranged from 24.8 to 77.4 mg/100g; Contents of the same mineral element of different pear varieties were different.

不同梨品种果实营养成分含量的分析结果表明：果糖、葡萄糖和蔗糖是梨果实中的主要糖类，品种间三种糖的含量有较大差异，所测品种果实中三种糖的含量范围分别为：3.40%～9.24%、0.21%～2.72%、0.10%～1.69%；可滴定酸的含量以八月红最高(0.286%)，爱宕最低(0.078%)；维生素C的含量以博斯克最高(9.50 mg/100g)，绿宝石最低(1.89mg/100g)；不同品种间可溶性蛋白和氨基酸的含量差异较大，其含量范围分别为127～428mg/100g和77.4～24.8 mg/100g；不同品种间同一矿质元素的含量存在较大差异。

On the foundation of standard UKF algorithm, the ratio of the theoretical value to the practical ones of the innovation's covariance was taken as the input of the FLAC to reduce the dependency of FLAC on the filter model, the generality of the fussy adaptive UKF algorithm was intensified; In the procedure of adjusting the measurement noise's covariance dynamically, the adjusted range could be amplified or minified to different extent by setting the exponential adjusting parameter, so the adjusting velocity and precision of the algorithm was controlled.

在标准的非线性UKF算法基础上，以残差的实际方差与理论方差的比值作为FLAC的输入，使FLAC对滤波模型的依赖性减弱，强化了模糊自适应UKF方法的通用性；在对量测噪声方差阵进行动态调节的过程中，通过设置指数调节参数可不同程度地放大或缩小调节的幅度，使算法的调节速度和精度得到控制。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。