可氧化性

Objective:With environmental pollution and invasion of the inflammation induced by some materials, bronchia asthma and chronic obstructive pulmonary disease have become the important social public problems in recent years.

近年来，在支气管哮喘和慢性阻塞性肺疾病的治疗药物中，肾上腺皮质激素可对抗炎症或过敏介质，通过对慢性阻塞性肺疾病者的前炎症因子、炎症细胞、氧化应激的影响而发挥良好的疗效。

It can decompound omethoate with high efficiency and even survive under 3g/L omethoate. The degradation was related to the trend of the growth of the strain dynamically.

通过对NWB-36有机磷农药降解特性的研究，结果表明，该菌对有机磷农药的降解具有遗传稳定性、广谱性和高效性，可耐受3g/L的氧化乐果，降解呈现动态变化，与菌体的生长趋势呈现一定的相关性。

Liquid-phase epoxidation of allyl chloride with hydrogen peroxide was carried out over Ti-MWW, a novel titanosilicate with the MWW structure, to synthesize epichiorohydrin selectively and effectively. Ti-MWW proved to be superior to the conventional TS-1 catalyst in both catalytic activity and product selectivity. Ti-MWW prefers aprotic solvents of acetone and acetonitrile, both of which can restrain the solvolysis of epichiorohydrin, whereas TS-1 favors a protic solvent of methanol.

研究了具有MWW结构的新一代钛硅分子筛Ti-MWW催化剂对烯丙基氯液相环氧化高效合成环氧氯丙烷的催化性能结果表明，Ti-MWW的催化活性以及产物选择性均优于传统的钛硅分子筛TS-1，对于Ti-MWW，合适的溶剂为非质子性溶剂丙酮和乙腈，在该溶剂中环氧化物不易发生溶剂化开环反应；而对于TS-1，合适的溶剂是质子性溶剂甲醇，但甲醇可导致醇醚副产物的生成。

Based on relative literatures,this part has included five resesearch aspects as below.1 Fifteen compounds were isolated and purified by extraction,column chromatography,and their structures were determined on the basis of spectral analysis: acteoside(Ⅰ-1),isoacteoside(Ⅰ-2),crenatoside(Ⅰ-3),cistanoside F(Ⅰ-4),sinapoyl-4-O-β-D-glucoside(Ⅰ-5),adenosine(Ⅰ-6),β-siterol(Ⅰ-7),oleanic acid(Ⅰ-8), succinic acid(Ⅰ-9),caffeic acid(Ⅰ-10),protocatechuic aldehyde(Ⅰ-11),p-hydro xybenzyl alcohol(Ⅰ-12),β-daucosterol(Ⅰ-13),D-galacitol(Ⅰ-14),D-mannitol(Ⅰ-15).Ⅰ-4～15 were obtained from this plant for the first time,andⅠ-6,7,9,and 13 were isolated from Orobanche genus for the first time.2 The scavenging test of DPPH showed that most compounds have comparative antioxidant activity as L-ascorbic acid and part of them show better activity such as the O.coerulescens extract and phenylethanoid glycosides.Acteoside showed potent free radical scavenging effects with a median inhibition concentration of 25.6μg/ml.3 The anti-HBV activities of acteoside,isoacteoside and crenatoside were measured,and all of them showed suppressive activity on the expression of HBsAg and HBeAg in the HepG2.2.15 cell line.

本论文在文献调研基础上对紫花列当化学成分及生物活性进行了研究，并从免疫抗病毒角度探讨紫花列当中特征性成分类叶升麻苷的肝保护作用及其机制。1采用大孔树脂、硅胶和Sephadex LH-20等色谱技术对紫花列当进行系统的植物化学研究，从中分离得到19个化合物，利用UV和NMR等波谱手段及理化性质鉴定了其中的15个化合物，分别为类叶升麻苷(Ⅰ-1)、异类叶升麻苷(Ⅰ-2)、crenatoside(Ⅰ-3)、cistanoside F(Ⅰ-4)、sinapoyl-4-O-β-D-glucoside(Ⅰ-5)、腺苷(Ⅰ-6)、β-谷甾醇(Ⅰ-7)、齐墩果酸(Ⅰ-8)、琥珀酸(Ⅰ-9)、咖啡酸(Ⅰ-10)、原儿茶醛(Ⅰ-11)、对羟甲基苯甲酸(Ⅰ-12)、β-胡萝卜苷(Ⅰ-13)、D-半乳糖醇(Ⅰ-14)和甘露醇(Ⅰ-15除化合物Ⅰ-1、Ⅰ-2和Ⅰ-3外，其余化合物均为首次从该植物中分离得到。2)通过大孔树脂富集该药材有效部位苯乙醇总苷，并采用HPLC测得其所含特征性成分类叶升麻苷的含量可达80%以上。3DPPH自由基清除试验显示紫花列当提取物及其所含的苯乙醇苷类化合物均具有较好的抗氧化能力，其清除DPPH自由基能力接近于抗坏血酸。4采用卡介苗整体致敏、脂多糖离体攻击构建大鼠原代肝细胞免疫性损伤模型，在体外观察类叶升麻苷的保肝作用。

To synthesize the results of the research above, we found that the well result of the study on activities and detergency are extracted by methanol extracts. It had ability of antioxidant and anti-iflammatory activity on the biological activities. Sapindus mukorossi were surface activity well and stretched less damage on hair. It will raise application value of Sapindus mukorossi on cosmetics.

综合以上之结果，甲醇萃取物为乾燥粉末可增加原料保存性，且发现在生物活性及清洁力试验上皆以甲醇萃取物之效果最好，具抗氧化、抗发炎能力，此外存在表面活性优良性质，对头发强度之伤害性小，因此增加其在化菻~上之应用潜力。

It is suggested that mechanisms protecting epidermis of squamata species from UVB damage may involve in (1) Physical defensive mechanism: the death epidermal layer limited UVB transmitting to the viable cells;(2) Biochemical defensive mechanism: up-regulated CAT may protect skin from reactive oxygen species which may induced by UVB. Moreover, the lipid and proteins in the β-, meso- and α-layer may absorb incident UVB and formed damaged products that were limited in the original site;(3) Cellular defensive mechanism: quick initiating of proliferation of the germinal cells and forming of the new epidermis and shedding out the damaged epidermis shortened the damage process and restricted the damage area.

1物理性防御：角质层和中层阻挡绝大部分UVB透过，最大限度地减轻生发层的损伤；(2)生化性防御：通过上调CAT活性以减轻LVB诱导的活性氧对表皮的损伤，同时角质层蛋白质和中层脂类可吸收UVB，且形成的有害产物被局限于原位；(3)细胞性防御：通过迅速启动再生修复过程形成新的表皮，及时蜕去积累了脂类过氧化产物、受损蛋白质和晒斑细胞等的受损表皮，有效防止损伤的持续和蔓延。

Calcium, blood calcium decreased, the brain caused by increased nerve excitability plant, resulting in baby night waking, night terrors, night irritability, sleep; baby should be given calcium and vitamin D and more sun: A, calcium: elemental calcium daily 200-300mg (1) Composite Calcium Granule: 2-3 packets a day (each contains calcium 100mg)(2) the activity of calcium granules: 4-6 packets per day (calcium per tablet 50mg) B, fill D : daily 400-800IU (1) concentrated cod liver oil drops: 2-4 drops per day (2) vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IU attention these objects must be a doctor's prescription, prescribed application. C, to the sun to vitamin D, ultraviolet radiation to the skin 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day outdoors on the sun for half an hour each afternoon. 3, bile is a yellow pigment bilirubin, was it yellow staining and faeces, after bilirubin by oxidation into biliverdin, thus making green manure, the manure discharge encountered in diapers in the air oxygen, so that oxidation of bilirubin biliverdin, so that the surface of green manure.

缺钙、血钙降低，引起大脑植物性神经兴奋性增高，导致孩子夜醒、夜惊、夜间烦躁不安，睡不安稳；应给孩子补钙和维生素D并多晒太阳：A、补钙：每日元素钙200-300mg(1)复合钙冲剂：每日2-3包(每包含钙100mg)(2)活性钙冲剂：每日4-6包(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日2-4滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IU注意上述物均须医生处方，遵医嘱应用。C、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。3、胆汁中胆红素是一种黄色色素，被它染色及粪便呈黄色，胆红素经氧化过后变为胆绿素，从而使粪便呈绿色，排出的粪便在尿布中遇到空气中的氧，胆红素使氧化成胆绿素，使粪便的表面呈绿色。

In this study, LDL was oxidized by copper ion, 2, 2'-azobis(2-amidio-propane)dihydrochloride or macrophages in the absence or presence of apoH to delineate the correlation between apoH and LDL peroxidation,. It was shown that the formation of thiobarbituric acid reactive substances and conjugated dienes was decreased and the electrophoretic mobility of LDL was reduced in the presence of apoH. It suggests that apoH exerts an antioxidative effect.

为了进一步了解脂蛋白元 H 的表现与低密度脂蛋白氧化之间的关连性，分别以铜离子、 2， 2'-azobis(2-amidio-propane)dihydrochloride (一种可生成自由基的化合物)或巨噬细胞等来氧化低密度脂蛋白，发现在有脂蛋白元 H 的存在下，不论是 thiobarbituric acid reactive substances、共轭双烯的生成以及低密度脂蛋白电泳移动速率，都受到明显抑制。

The results showed that Acidithiobacillus ferrooxidans could effectively solubilise realgar and 16.3% arsenic dissolution could be achieved in 30 days under the conditions of pH 1.8 in Iron free 9 K medium (with additional 1.0g of ferrous iron per liter and 0.5% of realgar), 150 rpm, 30°C. The trace elements of Zn, Cu, Mg, Mn, Fe and Se et al. could also be effectively leached.

实验结果表明，氧化亚铁硫杆菌能够有效溶解雄黄中不溶性的硫化砷，30 ℃，pH1.8，以20%接种量接种驯化后的氧化亚铁硫杆菌BY-3条件下135r/min摇瓶浸出30天砷的溶出率可达16.3%，Zn、Cu、Mg、Mn、Fe、Se等有益微量元素都能够得到有效浸出。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。