可染色的

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The pigment carbon black our factory researches and produces is divided into three big series of high, middle and low, which is widely used in dyeing industry, such as plastic cement coating, the fiber staining, painting, the printing ink, the printer inked ribbon, the leather coloring, the papermaking, defferent kinds of pen industry, matching colors, mixing colors and so on, and it can be used in making scattered products such as the color cake, the color thick liquid,etc.

我厂研发生产的超细色素炭黑，分高、中、低三大系列，广泛用于塑胶涂料、纤维着色、油漆、油墨、打印机色带、皮革染色、造纸、各种笔业、配色、颜料、调色等染色业，并可用于予制色饼、色浆等予分散产品。本公司秉承"质量第一、诚信至上、周到服务、合作共赢"的宗旨，用优质的产品搭建供需桥梁、用热情服务构筑合作平台、用至诚至信密切商业伙伴，在互利互惠同谋发展的甚础上共创双方美好的明天！

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

In double labell the high contrast substrates of enzym should be used.

因而作者认为，在进行胰岛内分泌细胞的双重免疫组化染色时，一染后可经PBS漂洗后直接进行第二种抗原的免疫组化染色，既节省时间，又减少了因不同的化学试剂的应用而影响第二种抗原活性的可能性。

During the surgery, the lesion was found to be a solid tumor. A surgical wedge biopsy was done and purulent dark yellow fluid was drained from the center of the mass. Histologically, there was a solid pattern of rhabdoid and anaplastic round or oval cells haphazardly arranged with poor differentiation. Immunohistochemical staining was positive for vimentin, a mesenchymal marker; the reticulin staining was consistent with carcinoma.

我们实施了手术切片及肿瘤中心脓状物引流，病理组织学上，我们发现了不规则或未分化的横纹状、圆形及椭圆形细胞；免疫组织染色上，细胞广泛的对非上皮标记vimentin呈阳性反应、且其reticulin染色呈现出腺癌的形式；肉瘤及腺癌的特色皆可在切片中看到，所以我们的诊断是类肉瘤性线癌。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

The result shows that 8% BCPA, weak acid dye , proper temperature and pH value meet good dyeability for polypropylene .

结果表明，当BCPA含量为8%，选用弱酸性染料，适当控制染色温度和染浴pH值就可获得良好的可染性。

In quality management, technical testing laboratory with advanced computer color measurement instrument and automatic batching machine, drip feeder to ensure that the staining formula is reasonable and accuracy, with 15 computer-controlled high temperature and high pressure dyeing small prototype can completely simulate the actual production, thereby ensuring that the character color.

在质量管理方面，技术检测化验中心拥有先进的计算机测色仪和自动配料机，滴料机以确保染色配方的合理性和精确性，拥有15台计算机控制的高温高压染色小样机可完全模拟实际生产，从而确保色纱的品性。

It features more flexibility, high automation, constant tension and speed, low-tension dyeing etc. The AC converting servo system, PLC and large sc9 reen man-machine interface are adopted to make the machine operate automatically and simply.

自动化程度高，自动恒张力、恒线速运行，可适应低张力染色，高精度的交流变频伺服调速系统，可编程控器控制全机自动化操作，大屏幕人机面监控，操作简便，运行稳定可靠。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。