可染色的

Results:Whith the time and doses of ART incubation extended, ART significantly inhibited the proliferation of SGC-7901 cells and the inhibited effect shows dose-and -time-dependent. Obvious changes of apoptotic morphology were observed by invert microscope, fluorescence microscope, scanning electron microscope and transmission electron microscope, they showed that cells had marked nuclear condensation or the fragmentation of chromation as well as apoptotic , mitochondrial edema and vesicle , with the time of incubation extended , the proliferation rate become slow and the volume became small and transformed. FCM assay indicated that most of the cells were arrested in Go/Gi and the apoptotic peak appeared. During the prolong of incubation time, the apoptosis rate was increased. At the same time, the number of S and G2/M phase cells were decreased. The result of TUNEL indicant that there are apoptosis and necrosis.

结果：随着药物浓度的增加和作用时间的延长，蒿甲醚对胃癌SGC-7901细胞的抑制作用呈时间和浓度依赖关系：在倒置显微镜、荧光显微镜、扫描电镜和透射电镜下可观察到典型的凋亡细胞的形态改变，表现为：核固缩或染色质边聚或凝聚成大块状，可见凋亡小体，线粒体肿胀增殖，严重的空泡化，随作用时间的延长，细胞增殖速度减慢，细胞体积缩小变形；流式细胞术显示胃癌SGC-7901细胞出现明显的凋亡峰，随着作用时间的延长，其凋亡率逐渐升高，细胞周期阻滞在G_0/G_1期，S及G_2/M期细胞数大量减少；流式细胞术TUNEL检测结果显示细胞凋亡和坏死同时存在。

One such novel idea is that of inserting into the chromosomes of plants discrete genes that are not a part of the plants' natural constitution:specifically,the idea of inserting into nonleguminous plants the genes,if they can be identified and isolated,that fit the leguminous plants to be hosts for nitrogen-fixing bacteria.

这其中的一个新奇思想就是，在植物的染色体内注入并非是该植物自然构造一个部分的那些不相关联的因基：具体而言，这一思想是，在非豆科植物内注入这样一些基因，倘若这些基因可被辨识出来并被分离开来，而这些基因业已使豆科植物宜于充当那些具备固氮作用的细菌的寄主。

One such novel idea is that of inserting into the chromosomes of plants discrete genes that are not a part of the plants' natural constitution:specifically,the idea of inserting intononleguminous plants the genes,if they can be identified and isolated,that fit the leguminous plants to be hosts for nitrogen-fixing bacteria.

这其中的一个新颖思想就是，在植物的染色体内注入并非是该植物自然构造一个部分的那些不相关联的因基：具体而言，这一思想是，在非豆科植物内注入这样一些基因，倘若这些基因可被辨识出来并被分离开来，而这些基因业已使豆科植物宜于充当那些具备固氮作用的细菌的寄主。

Along with science's and technology's development, the licorice use is getting more and more widespread, raw material purification becomes the licorice frost, the liquorice extract, besides have the medicine to affect, moves in the ink or the pigment, may cause the dyeing to be even, may use for to make the fire-extinguishing the foaming agent and the petroleum drilling fluid stabilizer, makes the candy, the cigarette and the medical industry seasoner.

随着科学技术的发展，甘草的用途越来越广泛，原料精制而成的甘草霜、甘草膏，除有药物作用外，调在墨汁或颜料里，可使染色均匀，可用来作灭火剂的起泡剂和石油钻井液的稳定剂，作糖果、卷烟和医疗工业的调味剂。

Fan YS,Davis LM,Shows TB.Mapping small DNA sequences by fluorescence in situ hybridization directly on banded metaphase chromosome.Proc Nat1 Acad Sci USA,1990,87∶6223-6227.5 Cherif D,Julier C,Delattre O,et al.Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy:Application to regional mapping of human chromosome 11. Proc Nat1 Acad Sci USA,1990,87∶6639-6643.6 Harai M,Suto Y,Kanoh M.

我们曾经介绍过一种荧光R显带的方法［10］，在此基础上建立了一种在显带染色体上直接观察杂交信号的新的定位方法：在细胞培养时，同时加入BUdR和Hoechst33258，PI染色后即呈现清晰的荧光R带带型，用彩色和全色黑白胶卷照相均可得到满意的结果，尤其适用于国内具有一般条件的实验室。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

RESULTS: Totally 57 rats enrolled in this study, all animals completed Hot pain allergic symptom could not be observed on rats in experimental group during the whole experimental process, but continuously appeared in discharge percentage of nerve fibers at the central end of experimental rat sciatic nerve was remarkably lower than that of the control group 6.79％Sympathetic postganglion nerve fiber budding was not obvious in the slices of entire L4-5 dorsal root neuroganglion of injury side in 6 experimental rats,and basket-shaped cell could not be observed, but sympathetic postganglion nerve fiber budding was obvious in 2 rats in control groups with altogether 41 basket-shaped cells observed.

电生理实验：实验组大鼠坐骨神经中枢端的神经纤维自发放电百分数显著低于对照组(6.79％，46 7％)。③背根神经节切片荧光染色结果：实验组6只大鼠损伤侧L4-5的全部背根神经节切片中看不到明显的交感节后神经纤维芽生，没找到篮状细胞，而在对照组2只大鼠损伤侧L4-5的全部背根神经节切片中可看到明显的交感节后神经纤维芽生，共找到篮状细胞41只。

In order to obtain viable tetraploids, which remains a major challenge worldwide, research on spindle organization and chromosome behavior was carried out mainly in zhikong scallop, Chlamys farreri, a typical commercially important species which is being cultured in North China Sea. Immunofluorescent technique and laser scanning confocal microscopy has been developed for observation of both microtubule organization and chromosome segregation. Orcein squash method was also used for chromosome observation. A series of complicated configurations of the meiotic spindle and associated patterns of chromosome segregation, including tripolar and seperated bipolar segregation, were found in developing eggs following the inhibition of the first polar body.

为了攻克可存活四倍体人工培育技术的国际性研究难题，本研究选取了我国北方海区的典型经济贝类栉孔扇贝为主要研究对象，建立了一套免疫荧光染色和激光扫描共聚焦显微技术，结合使用染色体地衣红压片观察技术，对第一极体受抑制的受精卵的减数分裂纺锤体装配、染色体分离动态进行了研究，发现了一系列特殊的纺锤体装配及相应的染色体分离行为，包括三极分离、独立二极分离等分离类型，从亚细胞水平上阐明了四倍体形成的细胞学机理。

One such novel idea is that of inserting into the chromosomes of plants discrete genes that are not a part of the plants' natural constit ution:specifically,the idea of inserting into nonleguminous plants the genes,if they can be identified and isolated,that fit the leguminous plant s to be hosts for nitrogen-fixing bacteria.

复杂修饰+插入+省略+倒装）这其中的一个新颖思想就是，在植物的染色体内注入并非是该植物自然构造一个部分的那些不相关联的因基：具体而言，这一思想是，在非豆科植物内注入这样一些基因，倘若这些基因可被辨识出来并被分离开来，而这些基因业已使豆科植物宜于充当那些具备固氮作用的细菌的寄主。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。