可染色的

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果：在同一标本上，不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况，二者的分布区域大致相同，神经元分化初期，较集中分布在核周附近，神经元分化成熟则分布于胞质及突起内。

HIRA may function in regulating chromatin structure, nucleosome reassembly and cell cycle progression by the interactions of its different domains with different proteins.

HIRA可通过其不同的结构域与不同的蛋白质相互作用，来参与调节核小体的组装、染色质的结构及细胞周期的进程等。

NGF had obvious cytotoxicity on these cell lines ,which shows to be dose-dependent, especially for MGC-803 and BEL-7404 ,their IC_(50) value at the 24~ hours are 49.9mg/L and 55.46mg/L ; Main features of apoptosis under fluorescent microscope include reduction in volume,nuclear chromatin condensation, intensification under jacinth fluorescence; The early apoptosis rate of pristine cells which detected by FCM increases with NGF dose increasing.

2NGF对4种人肿瘤细胞均有细胞毒性作用，且呈剂量依赖关系，尤其是对MGC-803和BEL-7404的抑制作用更强，作用24h的IC50值分别为49.9mg/L和55.46mg/L，荧光显微镜下可观察到MGC-803凋亡细胞的主要特点为：细胞体积缩小，染色质固缩，橘红色荧光增强；流式细胞仪检测到MGC-803细胞的早期凋亡率随NGF浓度的增高而增加，各浓度组与对照组的早期细胞凋亡率比较有明显差异(P＜0.01)。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有按摩明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数按摩明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

结果 (1)ATP对U937细胞的增殖有明显的阻抑作用，加药48h后增殖的抑制率可达43%以上；(2)ATP处理的U937细胞周期发生改变，G1期细胞数明显增多，ATP作用24h时G1期前出现亚二倍体峰——凋亡峰，凋亡细胞数为3.4%，作用48h时凋亡细胞数增多为22.7%；(3)ATP处理的U937细胞首先在核内染色质浓缩成半月形贴近核膜，逐渐向核膜外移动，进入胞浆内再移向质膜内外面，紧贴质膜外面再逐步脱离细胞体，进入细胞基质中，成为游离的凋亡小体。

To be hosts for nitrogen-fixing bacteria.

这其中的一个新颖思想就是，在植物的染色体内注入并非是该植物自然构造一个部分的那些不相关联的因基：具体而言，这一思想是，在非豆科植物内注入这样一些基因，倘若这些基因可被辨识出来并被分离开来，而这些基因业已使豆科植物宜于充当那些具备固氮作用的细菌的寄主。

One such novel idea is that of inserting into the chromosomes of plants discrete genes that are not a part of the plants' natural constitution; specifically, the idea of inserting into nonleguminous plants the genes, if they can be identified and isolated, that fit the leguminous plants to be hosts for nitrogen-fixing bacteria.

这其中的一个新颖思想就是，在植物的染色体内注入并非是该植物自然构造一个部分的那些不相关联的因基：具体而言，这一思想是，在非豆科植物内注入这样一些基因，倘若这些基因可被辨识出来并被分离开来，而这些基因业已使豆科植物宜于充当那些具备固氮作用的细菌的寄主。

Cobalt and its compounds are used in electroplating and colouring ceramics and glass and as lamp filament s, catalysts, a trace element in fertilizers, and paint and varnish driers.

钴和它的化合物可应用在电镀，陶瓷器、玻璃的染色，制备催化剂、颜料和油漆的干燥剂，以及用作灯丝、肥料中的微量元素等等。

Results After 2 weeks, the number of skeletal myoblasts reached 107 per millilitre. Immunocytochemical analysis showed that over 90% cells were stained positive for myogenin, a factor specific to skeletal myocytes.

结果 采用组织块培养法，2周后成肌细胞的密度可达107/ml，免疫细胞化学分析显示90%以上的细胞呈骨骼肌特异的myogenin抗体染色阳性。

These results indicated that HA and PmmA are both good bone graft substitutes for mastoid obliteration causing no structural or functional damage to inner ear and inducing no rejection or inflammatory reaction. PmmA, in particular, can be prepared as the occasion requires and can be solidified in a short time, making itself endurable and mouldable.

内耳连续切片，HE染色，光镜观察：实验耳耳蜗结构，形态正常，毛细胞排列整齐，〓泡内壁对HA和PmmA未呈现任何炎性细胞和巨噬细胞等，表明人造骨对内耳结构和功能无损害，不引起排斥或炎症反应，相容性好，是良好的骨组织代替材料，尤其是骨水泥可临时配制，能在短时间内象水泥一样凝固，具有一定的抗压性及成形性，是一种良好的乳突重建材料。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。