可染的

Wtp53 has obvious antiblastic effect towards Y79 cells, and the apoptosis rate of Y79 cells was the highest after transfection mediated by ultrasound-microbubble.

结论wtp53基因对RB瘤细胞具有较明显的抑制生长的作用；质粒+微泡+超声辐照，质粒+脂质体转染，以及质粒+超声辐照，均可促进Y79细胞的凋亡，但超声微泡介导的转染引起的凋亡率高于脂质体转染，而二者的凋亡率又明显高于质粒+超声转染。

Based on introducing the control principle of the jigger system, the analysis of control requirement for the system was achieved, and the control scheme was proposed. The construction of hardware platform based on programmable logic controller was built. The control strategies about the velocity-tension transmission system and the temperature of dyeing liquor were presented.

在介绍卷染机控制原理的基础上，对系统的控制要求进行了分析，并确定了控制方案，搭建了基于可编程逻辑控制器的卷染机硬件控制平台，提出了卷染生产过程中速度张力传动控制策略和染液温度控制策略。

The results showed that the acid releaser HTA was able to lower the pH values gradually and to increase the levelness without changing the dye fastness.

探讨了释酸剂用量、加入方式以及起始pH值等对锦纶弱酸性染料染色的上染速率和上染率的影响，结果表明：释酸剂HTA能逐渐降低染液的pH值，在不改变染色牢度的前提下，可提高匀染性

Methods In this study,a plasmid vector,rAd5,and rAAV2 were used to transfect rat cochlear marginal cells of stria vascularis by injection into the perilymph through the round window membrane,and the transfection efficiency, target tissue accessibility, cell/ tissue toxicity, time course of expression, and effect on hearing were evaluated in vivo and in vitro.

结果三种载体对原代培养的大鼠耳边缘细胞的转染研究发现，腺病毒的转染效率最高，而腺相关病毒转染对细胞活性影响最小。rAAV2和rAd5对大鼠耳蜗组织的感染实验发现，腺病毒和腺相关病毒携带的增强型绿色荧光蛋白可在多种耳蜗组织中及转染对侧耳蜗组织表达，且并不引起明显的耳蜗组织细胞凋亡。rAAV2携带的EGFP基因在第90天时仍可检测到大量表达。rAd5携带的EGFP基因在第30天时表达明显减弱。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

Suppression of metastatic hemangiosarcoma by a parvovirus MVMp vector transducing the CXCL10 chemokine into immunocompetent mice.

2.2 转染细胞表达CXCL10蛋白的检测采用Western blot分别检测转染CXCL10表达载体的细胞及其培养上清中CXCL10的表达，均可检测到CXCL10蛋白；而转染空载体组和未转染组细胞均未能检测到CXCL10存在

The research and development of levelling agent for vat dyes and cationic dyes was reviewed,which provide reference for the application, repreparation and development of levelling agent in China.

匀染剂具有缓染性和移染性，可有效改善染料的匀染性达到均匀染色的结果，从而提高染料的应用性能。

Dollars, with five pad assembly line, in dyeing and finishing capacity of 150 million meters, the company leading product for export cotton jacquard fabrics, real wax printed cloth, cotton, linen, canvas, plain cloth, T / C cloth, knitting cloth, etc., with singeing, calendering, silk-ming, pre-shrinking, super-soft, waterproof, sand , sanding and other finishing capabilities, export cotton jacquard fabric series, real wax printed cloth series has enjoyed a high reputation in West Africa, the company has become a major exporter of cotton jacquard fabric dyeing and finishing, dyeing and finishing of the leading linen large enterprises and exports, according to "China Bast net" Customs statistics show that: in 2006 January-August linen and ramie woven fabrics exports ranked Division I ranks No.

公司现有占地面积316亩，总资产10亿元，年销售近十亿元，自营出口约7000万美元，拥有五条轧染流水线，年染整能力可达15000万米，公司主导产品为全棉出口大提花布、真蜡印花布、棉布、麻布、帆布、平布、T/C布、针织布等，具有烧毛、轧光、丝鸣、预缩、超柔、防水、砂洗、磨毛等多种后整理能力，全棉出口大提花布系列、真蜡印花布系列已在西非享有很高的声誉，公司已成为国内全棉出口大提花布染整、麻布染整的龙头企业和出口大户，据《中国麻纺网》的海关统计显示：2006年1-8月亚麻及苎麻机织物出口排名中我司列全国第41位。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。