可杂交的

The new method combined the use of two DNA probes whose sequences comprises the whole complementary strand to the target DNA, in which one probe was labeled with an EDTA-〓 complex on the 5'-terminus and the other, labeled with a bidentate β-diketone on the 3'-terminus.

该法测定目标DNA的最低检测下限为6 pM，比现有其它均相DNA杂交分析法低2-3个数量级。该法具有简单、快速和高灵敏度等优点，通过使用各种的标记DNA探针，预计该法可广泛应用于各种实际DNA样品的分析。

The emasculation method is effective and rapid for carrot, and the heredit of important characters could be further studied.

因此，本方法可快速有效去除胡萝卜雄蕊，进行自交系之间的杂交，为深入开展优异基因的聚合及胡萝卜重要农艺性状的遗传规律研究奠定了基础。

Results Four hybridoma cell lines stably secreting McAbs against sporozoites were obtained: Easp-3G3 and Easp-5G10 belonging to IgG1, Easp-3H6 belonging to IgG2b, Easp-5H4 belonging to IgG2a. All four McAbs bound with E. acervulina sporozoite protein, but the Easp-5H4 McAb showed cross reactivity with E.

结果 获得4株可稳定分泌单克隆抗体的杂交瘤细胞株，其中Easp-3G3、Easp-5G10为IgG1 类，Easp-3H6为IgG2b 类，Easp-5H4为IgG2a 类。4株单克隆抗体均能与堆型艾美球虫子孢子的抗原蛋白反应，但仅Easp-5H4能与柔嫩艾美球虫子孢子抗原蛋白反应。

In the progeny of monosomic addition lines a high frequency of wheat-alien species translocation with various segments of chromosomes could be found by application of both PCR and GISH technique.

利用PCR和GISH原位杂交相结合的方法可提高检测外源染色体小片段的准确性和选择效率。

A novel double hairpin molecular beacon that has more stable structure than traditional molecular beacon has been designed. Its hairpin structure will not fully open when it hybridizes to cDNA but the hybrid can be cut by restriction endonucleases, which leads to destroy of its structure and restore the fluorescence.

本论文的主要内容包括以下三个方面：(1)基于双发夹分子信标分析核酸甲基化酶及药物筛选我们设计了新型分子信标-双发夹分子信标，该探针具有比传统分子信标更稳定的分子结构，与互补核酸链杂交后发夹结构不完全打开，但在酶切反应后其发夹结构被破坏，荧光又可恢复。

The two response models the thesis concluded could be not only applied in clinical detection,but also applied in the studies of hybridizing kinetics and thermodynamics.

论文推导出的两种响应模型不但成功地应用于临床检测，而且可用于杂交动力学及热力学的研究。

18 Days after perithecia appearance was the best time to isolate ascospores, although there were differences between crosses. The highest survival percentage of ascospores could reach up to 85. 5％ during this period.

观察发现，虽然不同杂交组合间有一定差异，分离稻瘟病菌子囊孢子的适宜时期，一般在子囊壳开始出现后7~18天之间；在此期间分离出的子囊孢子成活率最高可达85.5％。

Results 371 bp DNA fragment was amplified from 24 different species. The sensitivity could be improved to 10-12 g. No signal was observed when human DNA,funguses and viruses were used as templates. 22 blood samples and 4 cerebrospinal fluid samples, being positive with culture, were positive by using PCR. The gramnegative and grampositive probes hybridized to clinic samples and different species, as predicted by Gram stain characteristics.

结果 对24株不同标准菌株进行PCR扩增，均出现371 bp长度的DNA片段，敏感性试验可检测出10-12 g的细菌DNA，与人类基因组DNA、真菌及病毒无交叉反应；22例血培养阳性标本及4例脑脊液培养阳性标本均扩增出371 bp长度DNA条带，反相杂交法区分革兰阳性/阴性细菌与培养结果相符。

The distinction is, however, less clear cut than this suggests, since pidgins and Creoles characteristically operate on a 'post Creole continuum', which itself may be seen as a linguistic expression_r_r of the flux of HYBRID CONTACTS.

然而，自从洋泾滨语与混合语显著地作用于一种&后混合语的连续体&——其本身可被视为杂交接触之变化的语言表达——以来，区别已不是太明确。

The highest biomass and Zn content of the strain was obtained 30h at 28℃ and 220r/min, when 40mL of the culture in 250-mL shake flasks was grown in wert co...

在优化的条件下，杂交菌株ZGH 374的生物量达到 14 。3g L ，细胞锌含量可达到 9.3mg g ，锌总含量达到了 133mg L。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。