可杂交的

The decomposition of chromosome was a main problem in construction of BAC library. DNA discompose would be avoided or decreased by adjustments of component and pH of chromosome extraction buffer or pre-electrophoresis for 6-8h before digestion. Identification of inter-species hybrids was a necessary step for creating a new germplasm. Two samples were selected from offspring of hybridization and backcross of a cross between cabbage and Chinese cabbage.

鉴定种间杂种是利用远缘杂交创造新种质时的一项重要工作，本研究运用细胞核DNA含量流式测定技术，对甘蓝和白菜杂交及回交各世代的DNA含量进行检测，以监控遗传物质在不同材料间的流向，并从回交二代植株群体中筛选了两个材料，具有和白菜差异不显著的DNA含量，可推断其为核质杂种。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation on the 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF 的mRNA表达上升，损伤8天到达高峰，此后SDNSF 的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的 mRNA 阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

The immunohistochemistry showed that accompanied with SDNSF mRNA upregulation, the nestin-positive cells showed erupted roots, migrated peripherad and proliferation onthe 8-day slice.

结果 RT-PCR检测SDNSF mRNA在正常大鼠脊髓中的表达，损伤后4天SDNSF的mRNA表达上升，损伤8天到达高峰，此后SDNSF的mRNA表达逐渐减少，到16天恢复到正常水平；脊髓切片原位杂交结果发现SDNSF的mRNA阳性细胞主要分布于脊髓灰质细胞中，可能是神经元细胞，结果表明正常脊髓可表达SDNSF；脊髓损伤后8天，原位杂交显示SDNSF阳性细胞明显增多。

Results In the follicles of estrous cycle rat,the signals of MMP 2mRNA were detected in the granulosa cells and thecal cells;w...

结果 在动情周期中，各情期卵巢中卵泡的颗粒细胞和发育良好的卵泡的膜细胞中均可检测到MMP 2mRNA的杂交信号；MMP 9mRNA的表达始于已发育良好的窦前卵泡的膜细胞，此后在小窦状卵泡、窦状卵泡及排卵前卵泡的膜细胞中均检测到MMP 9mRNA的杂交信号。

Electrochemical cyclic voltammetry was employed to monitor the completion degree of hybridization, it was found that the the voltametric current was about 6 times increase under the microactuating with comparison of the non- microactuating.

用电化学循环伏安法监测其杂交程度，发现这种体积变化所致微点致动可引起DNA杂交度的很大增加，反映在伏安电流值上的变化是大约为无体积变化致动下的6倍。

The application of MAS in rice bacterial blight resistance and rice blast resistance breeding programs showed it is efficient in selecting individuals containing resistance genes from a crossing population, reducing the scale of subsequent experiment, and breeding variety with pyramided genes.

以抗白叶枯病基因及抗稻热病基因为例，利用分子标志辅助选拔进行杂交后裔族群的筛选，能有效地自杂交后裔族群中筛选出具有抗病基因的个体，并可缩小病原菌接种的试验规模，快速地育成堆叠抗病基因的品系。

Based on the practice of nectarine breeding,the systematical inheritance of peel tomenta, flesh color ,pollen fertility and flavor of parents and their hybridization offsprings was analysed.

结合油桃育种实践，对亲本、杂交后代的果皮茸毛、肉色、花粉育性、果实风味等性状进行了系统遗传分析，结果进一步证实了果皮茸毛、肉色、花粉育性3个性状各由1对等位基因控制，其中果皮有茸毛对无茸毛、白肉对黄肉、花粉可育对败育均呈完全显性，且这3对等位基因分别呈独立遗传，无互作效应；选择甜风味的品种作亲本，杂交后代出现甜油桃单株的比例较高。

The combinated utilization of ICH and ISH can greatly improve the detective efficiency of micro-metastasis in SLNs. The result detected by ISH arere, The ISH is more sensitive than the ICH method for the detection of micromeastasis.

通过免疫组化法和原位杂交法的联合使用，可明显提高乳腺癌SLN微转移的检出率，同时也证明原位杂交法是可靠的，比免疫组化更为敏感的技术。

The property of the hybridoma cell lines to secrete expected antibody was confirmed by ELISA and Western blotting analysis.

杂交瘤细胞产生的抗体可以作为相关实验的材料，杂交瘤细胞也可作为从事植物抗体研究时抗体基因的来源。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。