可接种的

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

Result: Rhesus BMSC can be got in mass by density gradient centrifugation.; Rhesus BMSC can adhere to PLGA fiber quickly after plant and its growth , division ,proliferration is good; Some cells arrange like rosary on PLGA fiber surface, some cells still form the catenate or flaky cell bridge joined among PLGA fiber.

结果：采用密度梯度离心的方法可得到大量增殖能力强的猕猴骨髓基质干细胞；接种后的骨髓基质干细胞很快粘附于PLGA纤维上，生长、分裂、增殖良好，在PLGA纤维表面形成串珠样排列，部分细胞还形成连接在PLGA纤维间的链状或片状细胞桥。

The best relative effect of cross-protection was found when spore concentration of Cladosporium cucumerinum was adjusted to 1×10^2 spores/ml for 48 h interval. And it was much better in resistant cultivars than in susceptible ones. Meanwhile, after challenged by Corynespora cassiicola the cucumber induced by Colletotrichum orbiculare produced great resistance to brown-spot, but that increased the development of anthracnose.

在黑星病菌对霜霉病菌的诱导作用中，以浓度为1×10^2个／ml的黑星病菌孢子悬浮液的诱导效果最好，诱导间隔期为48h黄瓜黑星病菌对霜霉病的交互保护作用最明显，抗性品种的交互保护作用明显好于感病品种；炭疽病菌可诱导黄瓜有效抑制褐斑病的发生，但挑战接种褐斑病菌后，却促进了炭疽病的发生。

Used the dual culture, germination of spores and inoculation experiment , the strains of penicillium sp. had the strongest antifungal effectiveness to cytospora sp .of populus , so the strains of penicillium sp. could continue research .

综合两点对峙培养、孢子萌发和人工接种试验的结果可以看到青霉属菌株对杨树烂皮病菌的拮抗效果最好，因此，可对青霉属进行进一步试验为开发新的生防拮抗制剂打下基础。

The introduction combines traditional Chinese and Western medicine below the experience of enteritis of noxiousness of fine ailment of remedial Tibet mastiff, in order to offer reference. 1 hair sick condition and clinical expression ill dog are 2~4 month more age young dog, majority is come on suddenly, anorexia or abandon absolutely, mental depressed, systemic symptom worsens quickly, produce acuteness sex vomiting and diarrhoea, puke is first feed rotten, afterwards is yellow or olive bubble mucus and hematic type thing, have diarrhoea dung has the sticky stiff thing of grey yellow, turn after for rare dung of effluvial shape of embedded mucous membrane, then shows hematic dysentery, because ill dog is acuteness vomiting and diarrhoea are rapid dehydration, eyeball cave in, temperature is shown two-way and calorific,℃ of the 40~41 at the beginning of disease, 1~2d falls it is normal to come, after 3~4d answer elevatory, die very quickly next, 4~5d of course of diseases, the temperature when on one's deathbed drops more to normal temperature the following. Bottom of stomach of dog of die in one's bed of 2 analyse check has haemorrhage sex inflammation, show cardinal, small intestine mucous membrane falls off, alvine wall attenuates, there is gules mucus inside, mix inside large intestine have show wine blood excrement and urine, there are a lot of haemorrhage places on mucous membrane, haemorrhage of rectum mucous membrane is more, mesentery lymph node enlargement, sometimes afterwards sends intussusception, alvine tangent plane bleeds, cardiac muscle is loose, color becomes weak, cystic plentiful, liver is qualitative fragile and brittle. 3 diagnose 3.1 epidemiology to diagnose this ill much hair at young dog, rather dog of epidemic disease have an inoculation feels the most easily, be affected directly or affect secondhand, basically pass enteron infection, this disease all can happen all the year round, but with cold...

下面介绍中西医结合治疗藏獒细小病毒性肠炎的心得，以供参考。1发病情况和临床表现病犬多为2~4月龄幼犬，多数为忽然发病，食欲减退或废绝，精神沉郁，全身症状急剧恶化，发生剧烈性呕吐和腹泻，呕吐物先为食糜，继为黄色或黄绿色泡沫黏液和血样物，泻粪有灰黄色的黏稠物，后转为恶臭的内含黏膜状稀粪，继而呈血痢，由于病犬剧烈呕吐和腹泻迅速脱水，眼球下陷，体温呈双向发热，病初40~41℃，1~2d降至正常，3~4d后又复升高，然后很快死亡，病程4~5d，临死时体温多下降至常温以下。2剖检病死犬胃底部有出血性炎症，呈深红色，小肠黏膜脱落，肠壁变薄，内有红色黏液，大肠内混有呈暗红色血液粪便，黏膜上有许多出血点，直肠黏膜出血较多，肠系膜淋巴结肿大，有时继发肠套叠，肠切面出血，心肌松软，颜色变淡，胆囊充盈，肝质脆而易碎。3诊断3.1流行病学诊断本病多发于幼犬，未免疫接种犬最易感，直接感染或间接感染，主要通过消化道感染，该病一年四季均可发生，但以寒。。。

Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.

将其植入恒河猴背阔肌的肌袋内，以未转染GFP的BMSC用同样的方法接种块状可吸收HA上，作为对照，术后6周取材，4%的中性多聚甲醛固定，塑料包埋，制作骨磨片PI染色在激光共聚焦显微镜下进行观测。

The optimal pH and inoculating quantity for the cypermethrin degradation were 7.0 and 10%, respectively.

菌株GF31降解氯氰菊酯的最佳pH值为7.0，接种量为10%，对浓度高达300 mg/L的菊酯仍可保持较高的降解活性。

The research on the choice of bacteria vaccine and its addition, improvement of preparation and fermentation technique suggests that the amino nitrogen level can reach that of 180 at the time of only 60 days in artificial inoculation and the production period can be shortened to two thirds.

对豆酱制曲菌种的选择、制曲工艺条件的优化、种曲添加量及发酵工艺的优化研究表明，多菌种人工接种生产豆酱中氨基态氮的含量在第60天时就达到了自然发酵180天时的含量水平，生产周期可缩短三分之二。

The emodin did not inhibited multiplication of TMV in infected leaf discs or infectivity of TMV-RNA in vitro. The infectivity of TMV recovered by dialysis from incubations with emodin in vitro has regained showed that the emodin did not direct viricidal effect. The emodin did not reduce infectivity of TMV when applied before TMV inoculation.

大黄素对粒体无形态破坏作用，与TMV混合后经过透析可恢复大部分的侵染活性，体外混合不影响TMV-RNA的侵染活性，并无体内抑制复制作用，预先接种也不影响TMV随后的侵染。

He results show that the virus regained infectivity, and the tannins did not direct viricidal effect. Tannins extracted from Polygonum perfoliatum or Archontophoenix alexandrae seeds showed strongly anti-TMV activities separately. TMV virions seems to be aggregated when TMV incubated with the tannins by electron microscope. It seems that TMV coat protein bind to the tannins based on PAGE. Tannins did not reduce infectivity when applied either before or after TMV inoculation. It is proposed that tannins extracted from the 11 species inhibited TMV by binding with TMV coat protein to interfere with recognition site essential for viral coat protein. Tannins extracted from Euphorbia hirta, P. perfoliatum, P. cuspidatum reduce infectivity when applied immediately in N.

MV分别与这11种植物单宁混合后，通过透析除去单宁后，可恢复大部分侵染力，表明单宁对病毒粒体并无直接毒害作用；TMV与杠板归和假槟榔种子中提取的单宁分别混合后，电镜下观察粒体呈现明显的聚集现象；TMV外壳蛋白分别与这两种植物单宁混合后，聚丙烯酰胺凝胶电泳中CP泳带滞后；单宁在病毒接种前施用未降低TMV对叶片的侵染能力；这些结果表明植物单宁对TMV抑制作用可能是单宁与TMV-CP结合影响病毒对叶片侵染位点的识别而降低TMV侵染性，而这种结合是可逆的。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。