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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

If i die tomorrow I'd be allright Dream Theater Because i believe That after we're gone The spirit carries on I used to be frightened of dying I used to think death was the end But that was before I'm not scared anymore I know that my soul will transcend I may never find all the answers I may never understand why I may never prove What i know to be true But i know that i still have to try If i die tomorrow I'd be allright Because i believe That after we're gone The spirit carries on Victoria:"move on, be brave Because i am no longer here But please never let Your memory of me disappear" Nicholas: Safe in the light that surrounds me Free of the fear and the pain My questioning mind Has helped me to find The meaning in my life again Victoria's real I finally feel At peace with the girl in my dreams And now that i'm here It's perfectly clear I found out what all of this means If i die tomorrow I'd be allright Because i believe That after we're gone The spirit carries on Hypnotherapist: you are once again surrounded by a brilliant white light.

如果我死在明天我就是所有权梦剧场因为我相信之后，我们正在经历精神继承从前我害怕死亡从前我认为死亡是完不过，那是以前我不害怕了我知道我的灵魂将超越我可能永远找不到所有的答案我可能永远不会明白为什么我可从来没有证明我所知道的是事实但我知道我仍然要尝试如果我死在明天我就是所有权因为我相信之后，我们正在经历精神继承维多利亚："的大举措，要有勇气因为我已不再在这里但请决不辜负你的记忆力对我的消失"尼古拉斯：安全，在轻环绕箱免费的恐惧和痛苦我的质疑态度已帮我找到意思是我人生的又维多利亚的实质最后，我觉得在和平与女孩在我的梦想现在我来这里它的完全清楚我发现什么所有这一切都意味着如果我死在明天我就是所有权因为我相信之后，我们正在经历精神继承

Late 2004, after a vacation in London and Italy respectively with my father and mother, my father opened a joint bank account of $9,700,000 US Dollars in a London bank on my name as the next of kin and inheritor, and on arrival to Senegal, i and my mother decided to transfer the money out of London after we were informed about the new British government's banking policy of deduction of taxes from funds and deposits in British banks that belong to non-residents which we saw as dangerous and highly exploitative, and after requesting for the modalities of transfer of the money out of London I was told to search for someone who will provide a bank account to pay in the money since I am currently refugees without oversea bank account.

他们雇佣了杀手，向我母亲开了两枪，我母亲死在了医院抢救室的手术台上。2004年末，我分别与我的父母在伦敦和意大利度过了两个假期，我父亲在伦敦的一家银行用我，下一个王位继承人，的名字开了一个联合帐户，里面有970万美金。到达塞内加尔之后，我们收到了英国新政府的金融政策的通知，他们将要从他们认为具有危险性和可剥削性的非本国居民的基金和存款中扣除税费，因此，我和我的母亲决定把我们的钱从伦敦银行中转出来。但当我请求转钱出来的时候，我被告知我需要找到一个愿意提供用于转入资金的帐户的人，因为我现在是一个难民，是没有海外帐户的。

Looky here, see, my eyes are dry No tears when I said goodbye Know better than to ask me why (Don't even try) Cause I'm gone, so gone Pack your bags, boy, it's time to carry on I'm taking back all that's mine All of the years that I gave All of these tears that I waste I think it's time that I get on with my life Although I might be alone, I'm better on my own.

我以为我告诉过你这是我独立的一天我找到一个全新的生活即使我的心在渴望可我会一小步一小步变得更坚强回头看看我留下的知道这个决定是对的你说你会在我身边现在看来你完全都不在乎我真的不想再承受了这些年来我所付出的那些我浪费的眼泪我想是时候往前走即使我会孤单一人我自已会过得好没有你在这里我过的比好还要好没有男人就没有哭啼看这里吧我的眼晴是干的说再见的时候我并没有哭想还知道吧那问我吧(Don't even try)根本不要哭因为我已经走了已经走了收起你的包男孩是时候向前走我所讲的都是我的这些年来我所付出的那些我浪费的眼泪我想是时候往前走即使我会孤单一人我自已会过得好没有你在这里我过的比好还要好没有男人就没有哭啼这些年来我所付出的那些我浪费的眼泪我想是时候往前走即使我会孤单一人我自已会过得好没有你在这里我过的比好还要好没有男人就没有哭啼 OO

The measured object can be found within a few milliseconds in the raster-scan image. 4 . Two lines delay for the processed image is implemented using two 128×8 shift registers with the configurable logic block in FPGA. A new fully pipelined bit parallel hardware architecture for Sobel edge detection is implemented with FPGA. Furthermore, it takes no more than 2ms to complete the edge detection of an image with 128×128 pixels.

视觉检测信号的前端处理技术 1、系统地分析了视觉检测算法层次性特点和计算特点，提出了提高视觉检测信号处理速度的基本方案：采用前端处理系统，自动识别被测物体在获取图像中的位置，并将真正有用的被测物体的边缘坐标值，提供给计算机，作进一步的高精度处理； 2、系统研究了实时图像硬件处理的设计基本方法，提出了基于FPGA的视觉检测信号的低级处理算法硬件实现模块化模型； 3、提出了一种新的基于FPGA的模板匹配并行模块化结构，解决了二维图像处理高带宽要求，在几毫秒内就可以在按扫描顺序输入图像中找到检测目标物体的位置； 4、采用FPGA内部的可配置逻辑块实现了128×8移位寄存器的设计，解决了大容量移位寄存器与FPGA内部寄存器数量之间的矛盾，实现了两行图像的延迟。

The main results in this paper as follows: First, a prototype of a shape-based image database retrieval system is completed, it can receive the query mode both by giving an example image and by sketching the desired object on the screen, Second, five shape-based image retrieval methods are realized; Third, an algorithm based on triangulation for shape-based image retrieval is brought forward. In this algorithm, firstly, the edge of the original image is followed and the candidate corners in the original image are detected. Then the counterpoints of the candidate corners in the result of edge follow are found, and the boundary corners whose counterpoints have been found are queued in the order of their counterpoints in edge follow. After these steps, the boundary corners are triangulated and triangles are obtained which can show the true shape of the original image. Finally, shape feature is obtained by calculating the angle histogram of the triangles.

同时本文中主要完成了以下三方面的工作：完成了一个基于形状特征的图象检索的原型系统，可实现例子图象或徒手绘草图两种查询方式；实现了五种利用形状特征进行图象检索的具体方法，并对其进行了分析比较；并且在继承将三角剖分引入形状检索的思想基础上提出了利用三角剖分进行形状检索的一种新算法：先对原图象进行边界跟踪和角点检测；然后寻找初始角点在边界跟踪中的对应点，并对找到对应点的角点按其对应点在边界跟踪中的顺序进行排序；再对排序后的角点进行德洛内三角剖分，得到能表示目标真实形状的三角形序列；最后计算三角形序列的角度直方图作为形状特征，并进行相似性匹配。

In many convents we find numerous instances of members of the families of the founders and chief benefactors becoming conventuals; in some cases the relationship of uncle and nephew may be traced through several centuries; just as the prebends of cathedrals and collegiate churches were often the gift of the founder and his family and were handed down from generation to generation, the more humble cells of a Carmelite convent remained frequently in the hands of one and the same family who considered it their duty as well as their right to be ever represented by at least one member.

在许多修道院，我们找到无数的事例家庭成员的创始人兼恩人，成为conventuals ，在某些情况下的关系，叔叔和侄儿最早可追溯到经过几百年；正如prebends的大教堂和合议教会往往是礼物的创始人和他的家人，并分别传世一代一代传下去，越谦虚细胞的一个carmelite修道院仍然经常在手中的是同一个家族，他们认为这是他们的职责以及他们的权利比以往派在至少有一名成员。

In the gold I do not know when to disappear, but regret is gold, I do not know is the gold or the ignorance of fools gold, or gold, the stupid, because the life of a people to get through, obviously have a look at light, but suddenly disappeared , this has been considered to be friends, the twinkling of an eye has become a fantasy, too naive individual gold each picked him as a diamond stone, but I do not know it was not a stone that suddenly disappeared, the stones told of his dream of gold , gold stone, he also told of the plans, may God缓过gold has not disappeared in the sea to stone, and gold found along the way that people had not thought of lost gold and lost a stone but it is also touch gold He was no light, he also lost the value of gold as a financial one for the mud Pakistan, gold has lost a stone has become a mud Pakistan, and Pakistan that the mud has to be done Have you ever thought of mud Pakistan, and Pakistan has no mud to become the sun above the earth, he knew that one day, sooner or later will become the sun, gold may be done, and may not do, you may also be less than a lifetime, then how do find a rock, or you have to rely on their parents ?

在金子不知到的时候消失，却是让金子感到后悔，不知是金子的无知还是金子的傻，还是金子的笨，一辈子就因该一个人度过，看着明明中有了光芒，却突然消失，本认为是有了朋友，转眼却成为了幻想，金子个人太天真了，每次捡到石头就把他当做钻石，却不知那是不是突然之间那石头消失了，石头告诉金子他的梦想，金子也告诉了石头他的计划，可在金子还没有缓过神来石头消失在海里了，金子以为找到了一路人，却不曾想过要失去，一但金子失去的石头，那摸金子也就没有他原来的光芒，也就失去了他的价值，金子一为财务一为烂泥巴，金子失去了石头就成为了烂泥巴了，那这个烂泥巴有没有想过一直要做烂泥巴了，没有烂泥巴一直想要成为地球上面的太阳，他知道迟早有一天会成为太阳，金子可能作到了，可能没作到，可能一辈子也作不到，那摸石头怎么办了，还是一直依靠父母吗？

A wide selection of products from SPF 15 to SPF 50, from oil-free sport formulations to water resistant forms, from extra gentle kids' formulations to fragrance-free -- the entire product line-up ensures every member of the family will find the product to meet their own special sun care need.

整个产品系列包含广泛的产品可供消费者选择--从SPF 15到SPF 50，从不含油脂的运动配方到防水型配方，从适合孩子使用的超温和配方到无人工香料的配方--确保家庭的每个成员都能找到符合其专门防晒护理需求的产品。

This is true or cortical areas which in man have relevance to language; it is also true of Broca area.

近似人类的灵长类，其大脑皮质的细胞分布约略和人类有不同的界线，从人类皮质区域跟语言的相关性以及波卡区就可看出，不过在灵长类的波卡区细胞建构中，也可以找到大致与人类同源的皮质区域。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。