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Microcells were produced by centrifugation of micronucleated A9 (neo12) cells in Percoll density gradient containing 20 mg/L Cytochalasin B at 39 000 g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 μm and 5 μm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20 mg/L细胞松弛B的Percoll密度梯度介质中,经39 000g高速离心后,包含微细胞、完整细胞、细胞核和细胞碎片的混合液,依次通过8 μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

Microcells were produced by centrifugation of micronucleated A9 (neol2) cells in Percoll density gradient containing 20mg/L Cytochalasin B at 39 000g. The resulting mixture of microcells, whole cells, karyoplasts and cytoplast fragments was filtered through 8 pm and 5 pm size membrane pores sequentially to obtain pure preparation of microcells.

微核化的细胞在含有20mg/L细胞松弛B的Percoll密度梯度介质中,经39000g高速离心后,包含微细胞、完整细胞、细胞核和细胞碎片的混合液,依次通过8μm和5μm孔径的滤膜过滤后可获得纯化的微细胞溶液。

In addition, the reagent in O-T method are more toxic and irritative, so GOD method adopted in this paper.The stability of INS in digestive secretion was investigated. The results show that the degradation rate in gastric solution was so fast that any pharmaceuticalmodification and adding enzyme inhibitors could not protect it.

在肠道吸收部位的研究中发现,INS溶液在大肠处给药可有微量吸收,但能引起动物血糖的明显下降,INS的水溶液和微乳液的吸收与药效无明显差异;在小肠处,INS溶液不能吸收,也不能引起大鼠血糖的降低,而制成微乳液后,可经小肠粘膜吸收,并引起血糖的明显下降。

C/OS-Ⅱ,the Real-time kernel is a highly portable, ROMable, very scalable, preemptive real-time, multitasking kernel for microprocessors and microcontrollers.

C/OS-Ⅱ是一种可移植、可固化、可裁剪及可剥夺型的多任务实时操作系统,适用于各种微处理器和微控制器。

Basing on utilization of organiccarbon compounds,mixotrophic culture is expected to possess advantages such as aless light requirement,capability of adopting fermentation techniques ofheterotrophic microorganisms and achieving a high cell density.

以对有机底物的利用为基础的微藻混合营养培养具有可降低光的需求、可借鉴已有的异养微生物的培养技术和实现高细胞密度培养等优势,是微藻培养技术的最新发展之一。

To enrich the outlook of glass-ceramics and improve its competitive power, an ingredients schedule with fly-ash solid residue as main raw materials, which contained fluorine after some valuable materials were obtained, was put forward based on theoretic analysis, making use of the characteristics of silicofluoride as crystal nucleating agents.

为丰富微晶玻璃外观色泽,提高产品竞争力,利用氟硅酸盐在微晶玻璃制备中可作成核剂的特性,经过理论分析确定以粉煤灰提取有价成分后的含氟固体残渣为主要原料生产镁橄榄石微晶玻璃的配料。

Numerical simulation of micropolar fluids elastic lubrication with mixed friction in mechanical seal;2. The basic line contact elasto-hydrodynamic lubrication equations for micropolar fluids were developed on the basis of the theory of micropolar fluids.

应用微极流体理论,考虑流体的可压缩性,建立线接触微极流体动力润滑的基本方程,进行固液两相流体稳态流动弹流润滑数值分析,获得了润滑油膜压力、形状以及摩擦力分布,分析了微极参数对润滑性能的影响,并与不可压缩流体结果进行比较。

Since there are real demands to the fine powder of expansible graphite in the market, it was studied that glacial acetic acid were inserted into 2μm5μm micro-graphite using potassium dichromate, potassium permanganate, or ammonium persulphate as oxidant and acid as medium to get FPEG low expansible volume successfully.

针对目前对低膨胀容积的可膨胀微粉石墨的实际需求,本论文研究了在酸性条件下,以重铬酸钾、高锰酸钾或过硫酸铵为氧化剂,成功地将冰醋酸插入2μm5μm石墨层间,制得低膨胀容积的可膨胀微粉石墨。

A method for the determination of water soluble chloride content in iron ore by potentiometric titration was studied.

以2g/L硫酸钾为溶解试剂,硝酸银为滴定剂,电位滴定法测定铁矿石中的水溶氯,通过电极电位-浓度二次微商滴定曲线,计算二次微商等于零时对应的V为滴定终点,可省去绘制E-V曲线或ΔE/ΔV-V一次微商曲线作图带来的麻烦。

The 2 g/L solution of potassium sulfate was employed for the dissolving of sample, and silver nitrate was used as titrant.

以2g/L 硫酸钾为溶解试剂,硝酸银为滴定剂,电位滴定法测定铁矿石中的水溶氯,通过电极电位-浓度二次微商滴定曲线,计算二次微商等于零时对应的V为滴定终点,可省去绘制E-V曲线或ΔE/ΔV-V一次微商曲线作图带来的麻烦。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。