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The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

The measurement system error model analysis is introduced into power system real time network state analysis as an important part for the first time, which can improve on the state estimation quality and provide the capability to monitor the operation of the measurement system; 2. The theory and algorithm of the on-line estimation and update of measurement noise variance based on the relation between the residual variance and noise variance. The statistic properties of the sample variance are discussed and the relation between the estimation precision and sample size under given confidence level is derived; 3. The theory and algorithm of detection and identification of measurement bias are presented, which is based on the relation between residual mean and noise mean. The statistic properties of sample mean are discussed and the relation between estimation precision and sample size is derived; 4. The Givens orthogonal transformation algorithm is selected to be the essential algorithm of state estimation, the fast orthogonal transformation algorithm with damp factor and the algorithm which can handle the zero injection measurements efficiently are presented; 5. The quantity analysis theory of bad data detectivity and identifibility are presented, which describes the relation between the elements in matrix W〓 and bad data amplitude and can provide the theory base for measurement system design and valuation.

一、首次将量测系统误差模型分析做为一个环节引入电力系统实时网络状态分析中,为EMS系统增加了实时监视系统运行、修正量测系统误差模型的新功能,进一步发挥了实时网络状态分析应用软件的潜力;二、首次提出了应用样本方差在线估计与修正量测系统误差方差的基本理论,讨论了样本方差的统计性质和概率分布,推导出了样本容量、估计精度和置信度之间的关系,给出了在线估计与修正量测系统误差方差的算法;三、首次提出了应用样本均值在线检测与辨识量测偏差的基本理论,讨论了样本均值的统计性质,推导了样本容量、估计精度和置信度之间的基本关系,给出了在线检测与辨识量测偏差的算法;四、在状态估计算法设计中,以Givens变换算法做为基本算法,提出了快速正交变换阻尼因子法和可以有效地处理零注入量测的混合法,并对实时应用中的一些问题进行了讨论;五、提出了不良数据可检测性与可辨识性的定量分析理论,揭示了描述量测系统配置、网络结构与参数的残差灵敏度矩阵中的元素与不良数据的幅值在可检测、可辨识能力上的定量关系,为量测系统配置设计与评价提供了理论基础;六、综合国内外最新研究成果,采用自适应自回归预测技术和稀疏矢量技术,构造了较完善的不良数据检测与辨识算法。

By experimenting with solid-liquid separator in the treatment of sewage before it is discharged on three large scale pig farms, we come to the conclusion that solid-liquid separator is effective in gathering dung sullage, with about 2.9 t/h and the water content less than 55%. The average rates of removal of TS, COD, and BOD are 82.5%, 77.5% and 73.5%, respectively. ByKey words: FZ-12 solid-liquid separator; large scale pig farm; sewage treatment

为了了解FZ-12固液分离机前处理效果,对3个规模化猪场进行污水前处理试验,结果表明,FZ-12固液分离机能有效地收集粪渣,平均收集量为2.9t/h左右,粪渣含水率〈55%,对猪场污水的TS、COD和BOD平均去除率分别达到82.4%、77.5%和73.5%,应用该机处理猪场污水,增加相关投资约人民币5.3万元,收集的粪渣出售后,扣除运行成本,年可增收税利约人民币4.5万元,投资回报率达84.9%,约1.18年可收回投资,因此,应用FZ-12固液分离机处理猪场污水,具有广阔的应用前景。

This thesis deeply researches the J2EE security strategy on the application tier of enterprise system. The security strategy predigests the information security model and meets the security requirements expediently. But there are some disadvantages when the security requirements become more complex. The expositive security on the Web-Tier can not offer more information for authentication; there is no authentication for EJB-Tier to use, make the EJB-Tier depend on the Web-Tier; J2EE uses database or application server to manage the role information. Use database to manage the role information will increase the complex of enterprise application, and the way of application server will reduce the ability of migrate other J2EE platform. To solve these problems, the thesis designs an improved security strategy model.

本文从企业级系统应用层安全的角度出发,深入研究J2EE多层体系结构的安全策略模型。J2EE提供的安全策略简化了系统的信息安全模型,能够很方便地满足基于J2EE的分布式应用系统的安全需求,但是在面对更加复杂的安全需求时,J2EE安全策略在验证授权和角色管理中还存在不足之处:其在Web层的声明式验证机制无法为用户提供更多验证信息,可扩展性较差;在EJB层缺少业务逻辑层可供采用的验证机制,而是借助于Web层的验证机制,其耦合程度较高有悖于业务逻辑分离于表现逻辑的原则;在角色管理方面,J2EE采用数据库或者应用服务器来管理角色信息,其中,数据库管理角色的方式增加了应用开发的复杂度,应用服务器管理角色降低了系统的可移植性,两者都具有片面性。

Later, under the untypical parasternal four-chamber view which can thoroughly display the ostium of coronary sinus, the catheter was promoted further to the ostium of coronary venous sinus. Then the echocardiography technician adjusted the transducer of TTE in order to thoroughly confirm the position of the catheter tip. After confirming the position of catheter tip in the coronary venous sinus, the operator inserted the catheter into the coronary venous sinus for 3~4 mm farther. It was noted that if resistance was encountered by operator, the operation must be stopped, which was the same as x-ray fluroscopy as image guiding. TTE guiding His bundle elactric cahteter、high right atrium electric cahteter and right ventricular electric catheter in site: It is difficult using TTE singly since there are too many crossroads in inferior venous.

本研究结果显示,(1)单独应用TTE作为影像学引导进行冠状窦电极导管置放的成功率为93.8%~96.7%,并且应用TTE作为影像学引导放置专用冠状窦电极导管和普通电极导管的成功率和放置时间在统计学上无显著差异;(2)TTE作为影像学引导,除过度肥胖患者外,可完全替代X线引导进行冠状窦电极导管置放,包括鞘管和扩张管的放置、指引导丝在静脉而非动脉内的证实均可由TTE完成引导,且较X线C型臂有一定优越性;(3)TTE结合普通X线胃肠透视机作为影像学导引,可顺利完成心内电生理检查时导管的安全到位,而不需昂贵的X线C型臂,可将心内电生理检查扩大到广大的基层医院;(4)TTE较X线更易和更早期发现介入治疗的并发症如急性心包填塞等,对并发症的防治甚至挽救病人的生命至为重要;(5)动物实验研究表明,TEE可引导射频导管消融术时大头电极导管成功到位。

To pay attention to carry out effective cardiopulmonary resuscitation, hemostasis by compression and deep vein intubation rapidly in the initial stage of emergency. The carotid shunt tube plays an important role in severe carotid injury and re-establishment process. We use external jugular vein to transplant and re-establish,which are rung with artificial blood vessels for the long distance carotid injury and achieved the best result. We expose as fully as possible for the hepatic venae and the posthepatic post caval injury,and adopt effective methods to stop bleeding and repair. The application of blood shunt pump could raise the success rate for those severe patients. The self-double vastransplantation with both ends being all trousers-style one opening solve some problems such as there are no suitable caliber self-transplantation blood vessels and the artificial blood vessels are easy to occur obliteration for long-term and so on for the great or middle blood vessels.We use improved Fogarty duct and anterograde intubation to eliminate thrombus could eliminate thrombus effectively and avoid accessory injury for the deep vein injury and extensive thrombosis.

抢救初期要注意进行有效的心肺复苏、压迫止血和快速进行深静脉插管;颈动脉转流管在严重颈动脉损伤重建过程中有重要作用;对长距离颈动脉损伤用颈外静脉移植外环包人造血管重建,可取得最佳效果;对肝静脉及肝后下腔静脉损伤,要充分暴露,采用有效方法止血和修复,对病情极危重的凶险性出血者,血液转流泵的应用可提高成功率;对大、中血管损伤,两端均为裤式单开口自体双血管移植,解决了无适宜口径自体移植血管和应用人造血管远期易闭塞等问题;对深静脉损伤并广泛血栓形成者,应用改进的Fogarty导管和顺行插管取栓法,可有效取栓并避免了副损伤。

To pay attention to carry out effective cardiopulmonary resus-citation,hemostasis by compression and deep vein intubation rapidly in the initial stage of emergency.The carotid shunt tube plays an important role in severe carotid injury and re-establishment process.We use external jugular vein to transplant and re-establish,which are rungwith artificial blood vessels for the long distance carotid injury and achieved the best result.We expose as fully as possible for the hepatic venae and the posthepatic post caval injury,and adopt effective methods to stop bleeding and repair.The application of blood shunt pump could raise the success rate for those severe patients.The self-double vastransplantation with both ends being all trousers-style one opening solve some problems such as there are no suitable caliber self-transplantation blood vessels and the artificial blood vessels are easy to occur obliteration for long-term and so on for the great or middle blood vessels.We use improved Fogarty duct and anterograde intubation to eliminate thrombus could eliminate thrombus effectively and avoid accessory injury for the deep vein injury and extensive thrombosis.

抢救初期要注意进行有效的心肺复苏、压迫止血和快速进行深静脉插管;颈动脉转流管在严重颈动脉损伤重建过程中有重要作用;对长距离颈动脉损伤用颈外静脉移植外环包人造血管重建,可取得最佳效果;对肝静脉及肝后下腔静脉损伤,要充分暴露,采用有效方法止血和修复,对病情极危重的凶险性出血者,血液转流泵的应用可提高成功率;对大、中血管损伤,两端均为裤式单开口自体双血管移植,解决了无适宜口径自体移植血管和应用人造血管远期易闭塞等问题;对深静脉损伤并广泛血栓形成者,应用改进的Fogarty导管和顺行插管取栓法,可有效取栓并避免了副损伤。

Applications Construction of industrial commercial or residential air handling operations Suitable for operating temperatures up to 120°C with maximum air velocity of 10 m/s and static pressure of 50 mm water gauge Benefits Non-combustible (Tested to SABS 0177 Part V) Class I fire index (Tested to SABS 0177 Part III) Integrated system Reduces manufacturing, material and insulation costsroduct can be tailor-made to suit the application Can be used in conjunction with metal and flexible ductwork Modifications can be fabricated on site Can be pre-grooved or grooved on site Low thermal conductivity Non corrosive Excellent sound reduction Controls condensation

应用 工业商业或住宅建筑空气处理操作适用于工作温度与10米,最高风速可达120℃/ s和50毫米水表静压优点不可燃(经测试可SABS规格0177第五部分) I类火灾指数(经测试可SABS规格0177第三部分)集成系统降低制造,材料和绝缘costsroduct可以度身订造,以满足特定的应用可用于金属管道和灵活的结合修改可以在现场制造可以预先槽槽或网站导热系数低非腐蚀性出色的声音还原控制凝结

The lamps are available with two viewing angles. With an angle of 30 degrees by 70 degrees, the red HLMP-AD31-UX000 and HLMP-AD30-UX000 (with and without lead standoffs, respectively), and amber HLMP-AL30-TW000 and HLMP-AL31-TW000 (with and without lead standoffs) are generally used for long-distance viewing in applications such as traffic signals.With a viewing angle of 40 degrees by 100 degrees, the red HLMP-HD31-SV000 and HLMP-HD30- SV000, and amber HLMP-HL31- RU000 and HLMP-HL30-RU000 (with and without lead standoffs) are ideal for wide-viewing-angle applications such as stadium scoreboards and other message signs.

这一系列LED可提供两种视角:红色HLMP-AD31-UX000和HLMP-AD30-UX000(一个有支架,一个没有支架)及琥珀色HLMP-AL30-TW000和HLMP-AL31-TW000(一个有支架,一个没有支架),可提供30 x 70度视角,通常用于交通信号等长距离应用;红色HLMP-HD31-SV000和HLMP-HD30-SV000及琥珀色HLMP-HL31- RU000和HLMP-HL30-RU000(一个有支架,一个没有支架),可提供40 x 100 度视角,适用于露天大型运动场的记分板和其他信息标志等宽视角应用领域。

On the research, firstly the principle of market forecasting and the strategy model of M.E.

应用了市场预测原理和波特战略模型等进行了运营环境研究,应用了货币时间价值理论对财务效益进行了研究,应用了盈亏平衡和敏感性分析理论对抗风险能力进行了研究,应用了可持续发展、循环经济等理论对可持续性发展能力进行了研究,应用了加法模型对项目的综合分析评价进行了研究。

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Shop assistant: Please check at the door.

店员:请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后,对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论,结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。

Quick stop! AIDS doctors while the side and say hello and colleagues mention a blood sample box, the Red Cross had sent blankets and so on, with patients有说有笑to go home.

快停车!防艾医生们一边招呼一边和和同事们提着血液采样箱,抱着红十字会送来的被子等,有说有笑地同患者往家走。