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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Incredibly high levels of cocaine — usually coupled with the abuse of other drugs — can lead to premature labor, preterm birth and low birth weight, Stanwood said.

令人难以置信的高水平可卡因-通常加上滥用其他药物-可导致早产,早产和低出生体重,s t anwood说。

It may also be combined with proptosis as a result of impaired digestive organs the body of waste discharge problems causing bowel is full then the fish will become even more swollen.

另外还可合并有眼球突出以及因消化器官功能受损、体内废弃物排出困难导致的肠满,这时鱼会变得更为膨大。

Pseudorabies is an infectious disease that primarily affects swine, but can also cause a fatal disease in dogs with signs similar to rabies.

伪狂犬病是一种最初感染猪的传染病,但也可导致犬死亡,其症状类似狂犬病。

Pyemia is caused by making a sneak attack the body-wide inflammation response syndrome which the infection causes. And further development may cause multi-organ function barrier syndrome. How to prevent and reduce pyemia is important.

脓毒症是由于侵袭性感染引起的全身性炎症反应综合征,进步发展可导致器官功能障碍综合征怎样预防和降低脓毒症具有重要意义。

In 1997 carried out an experiment in Canada found that a large number of saccharin sodium intake can lead to bladder cancer in male rats.

在1997年进行了一次实验中发现,加拿大的糖精钠大量摄取可导致雄性大鼠膀胱癌。

Both ischemia and ischemia/reperfusion injured the structures and functions of the membranes of sarcolemma and mitochondria.

两种损伤皆可导致大鼠心肌肌纤维膜及线粒体膜的结构和功能障碍。

Ladder outflow obstruction secondary to BPH can decrease the densities of cholinergic nerve and receptor and damage the function of cholinergic nerve in bladder detrusor.

PH引起膀胱出口梗阻,梗阻可导致逼尿肌胆碱能神经和受体的密度降低,以及胆碱能神经功能减退。

In those with sickle-cell anaemia or other abnormalities of the haemoglobin parvovirus B19 can cause an acute, severe anaemia and they may need to be admitted to hospital and to receive blood transfusions.

在这些与镰状细胞贫血或其他异常的血红蛋白微小病毒B19 ,可导致急性,严重贫血症,他们可能要入院接受治疗,并接受输血。

The disadvantage of forcing is that it can lead to stalemate if the other side uses the same approach.

缺点是,它可导致强迫僵持如果对方用同样的方法。

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