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Results Approximately 700 protein spots were revealed in equivalent proteins of the promastigotes and axenic amastigotes separated by 2-DE,among which more than 90% protein spots showed equivalent quantity and distribution,with 6 proteins up-regulated and 3 proteins down-regulated in axenic amastigotes compared with promastigotes.

结果 等量的前鞭毛体与纯培养的无鞭毛体总蛋白经双向电泳分离后均可获近700个蛋白点,其中超过90%的蛋白点的分布和相对强度基本一致。

The purity of Monochloroacetone can be reached to 99% by azeotropic distillation in the ratio of water to the mixture of Monochloroacetone and 1,1-dichloroacetone 1:1.

实验确定共沸剂水的加入量与氯丙酮混合液的比例为1:1时,能达到良好的分离效果,可得到纯度99%以上的一氯丙酮。

A compound 2-(3-hydroxyphenyl)[60] fullerene pyrrolidine was synthesized by the 1,3-dipolar cycloaddition reaction of the fullerene(C60) and azomethine ylide (prepared from glycine and 3-hydroxybenzaldehyde). The product was characterized by UV-Vis,1H-NMR ,FT-IR and MS. The reaction conditions affecting the yields of product were investigated and the optimum reaction conditions with product yield 75%(based on consumed C60) were:molar ratio of C60∶3-hydroxybenzaldehyde:glycine 1∶3∶6,reaction temperature 95℃,reaction time 18h.

利用甘氨酸和3-羟基苯甲醛与C60发生1, 3-偶极环加成反应,合成分离得到了2-(3-羟基苯基)[60]富勒烯吡咯烷,用UV-Vis、1H-NMR、FT-IR、MS等测试手段表征了产物的结构,并通过单因素方法,探讨了反应条件对产物产率的影响,得到最佳工艺条件:反应物摩尔比1∶3∶6,温度95 ℃,反应时间18 h,产物的产率可达75%(以消耗的C60计)。

The optimal measuring conditions were investigated. The results indicated that the catalytic reaction to Pt and bromocresol purple was the first-order reaction. The apparent activation energy was 217.4kJ/mol and the maximum absorption wavelength of fading reaction was at 434 nm. The linear range of Pt was 0.16-4.0μg/L and the detection limit was 2.5×10^(-11)g/mL. Most of common ions had no interference with the determination, while ten multiples of Ru, Rh and Os had interference with the determination of Pt. Therefore.

选择了最佳测定条件,实验结果表明,催化反应对铂和溴甲酚紫为一级反应,反应的表观活化能为217.4kJ/mol,褪色反应的最大吸收波长为434nm,方法测定R哪的线性范围为0.16~4.0μg/L,检出限为2.5×10^(-11)g/mL,大多数常见离子不干扰测定,10倍以上的Ru,Rh和Os对测定有干扰,可在测定前分离除去。

Identification of these cells: Over 90% cells isolated from human fetal calvaria were stained positively for both alkaline phosphatase and Alizarin red.

采用酶消化后植块的改良植块培养法可在较短时间内获得大量成骨细胞,这些细胞具有典型的成骨细胞形态和功能,是一种较好的分离培养人胚成骨细胞的方法。

The main steps include as follows: CO2 is led into the mother liquor of sodium meta-aluminate to carry out carbon content decomposing; the primary separation between the aluminum and the gallium is realized by primary carbonatation to filter out sodium hydroxide; secondary carbonatation is carried out on the filtrate to obtain the deposit of the complex salt of gallium and aluminum; a sodium carbonate crystal is obtained after the filtered filtrate is condensed and vaporized; the deposit of the complex salt of gallium and aluminum is dissolved in the mother liquor of sodium meta-aluminate; the processes are repeated until the proportion of gallium and aluminum in the complex salt of gallium and aluminum is larger than 1/340; then the complex salt of gallium and aluminum is added into the NaOH liquor and electrolyzed, thus obtaining the metal gallium with a purity larger than 99.9 percent which reaches the level of 3; simultaneously, the byproducts of the aluminum hydroxide and the sodium carbonate during the process of carbonatation by steps are obtained.

主要步骤包括:向铝酸钠母液中通入CO 2 进行碳分分解,一次碳酸化实现铝镓的初次分离;过滤出氢氧化钠,滤液进行二次碳酸化获得镓铝复盐沉淀,过滤后滤液经浓缩蒸发获得碳酸钠晶体,铝镓复盐沉淀溶解在偏铝酸钠母液中,重复上述过程直至铝镓复盐中的镓铝比例大于1/340,将其加入到NaOH溶液中,电解可获得纯度>99.9%的金属镓,达到3N级水平同时在分步碳酸化的过程中获得氢氧化铝和碳酸钠副产品。

By separating the crude oligosaccharide products with various ratios of activated charcoal and Celite, the results indicate that the products from 30% lactose (L30) had an adsorption percentage of 96.3%, the highest one among all mixtures of activated charcoal and Celite. The following fractionation by eluting successively with 10% and 20% ethanol aqueous solutions gave an oligosaccharide yield of 13.5% and a purity of 97.9%.

合成的寡醣以不同比例活性炭和矽藻土吸附分离,结果发现30%乳糖组(L30)经单活性炭组吸附即有96.3 %的寡醣吸附力,是所有活性炭与矽藻土混合组别中最高者,再经10 %与20 %乙醇冲提画分后可得13.5 %的收率,其寡醣纯度更高达97.9 %。

The effects of cholic acid concentration and pH of the buffer on separation were studied, and the influences of organic additives such as methanol, acetonitrile, isopropyl alcohol were also examined.

该实验表明电化学检测同样可用于手性物质的分离,从而扩宽了电化学检测的应用范围,并为对映体的定量合成和纯度测定找到了灵敏的检测方法。

The recoveries of seawater and sediment are :naphthalene 73. 0% and 75. 3%, phenanthrene79. 2%and78. 0%, fluoranthene 88. 1% and 80. 7%, pyrene 73. 8% and 70. 2%, chrysene 77. 6% and 72. 8%, benzofluoranthene 87. 6% and 83.6%, benzo pyrene 120. 3% and 110. 0%, respectively.

通过对7种多环芳烃标准样品的高效液相色谱法的定性、定量分析,结果表明,该方法可有效分离PAHs,回收率较高,海水和沉积物中的回收率分别为:萘73.0%和75.3%,菲79.2%和78.0%,荧蒽88.1%和80.7%,芘73.8%和70.2%,屈77.6%和72.8%,苯并荧蒽87.6%和83.6%,苯并芘120.3%和110.0%。

By use of AB-8 macroporous resin and with 10% gardenia yellow as adsorptive solution, selecting proper adsorptive and desorptive conditions, crocin and geniposide can be separated effectively, the purified gardenia yellow whose colority increases from 10 to 320 and OD ratio decreases from 2. 31 to less than 0.4 answers for the standard of gardenia yellow of high quality.

采用AB—8大孔树脂,以10%的栀子黄溶液上柱,选择合适的吸附、解吸条件,栀子黄的色价可从10提高至320,OD值比率也从2.31降至0.4以下,藏花素和栀子甙均达到了有效地分离。

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