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The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物,该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成,通过该引物可扩增片段大小为295bp,为了防止假阳性出现,本发明根据牛3号常染色体报道序列设计了一对内标引物C34,扩增片段大小为208bp,通过上述两对引物对单个牛精子进行双重PCR扩增,然后根据对检测结果的统计分析,对分离精子纯度做出最终评价。

The salt-out extraction process is used to deal with the waste acid from benzol refining .

试验采用盐析萃取工艺对焦化精苯废酸进行处理回收,结果表明,在一定的比例下可获得最佳萃取分离效果,酚相萃取液可进行二次利用,回收的净化酸可用于硫铵制备。

The thermodynamic parameters in the separation of enantiomers of amino acid derivatives and diastereomers of cinchona alkaloids were calculated and the dissociation constants of cinchonine and cinchonidine on the cinchonine imprinted monolithic disk were determined by frontal analysis.

计算了氨基酸衍生物对映体以及辛可纳非对映异构体分离过程的热力学参数并利用前沿色谱法测定了辛可宁和辛可尼丁在盘状分子印迹整体柱上的离解平衡常数。

T. , the isolated yield reached to 92% and the selectivity was above 99%. The product was immiscible with TSILs and could be separated simply by decantation after the reaction.

由于生成的乙酸苄酯不溶于催化体系,反应产物与催化体系分层,通过简单的倾析便可实现产物分离,简化了分离过程。

Results show this new-inlet hydrocyclone has original sides on liquid spin-making and stabilize its flow field. Moreover this gradual contraction inlet may translate some pressure energy of liquid into kinetic energy and increase circumrotate speed. These play active actions on improving separation efficiency. Moreover, the uniflow hydrocyclone has the traits of big capacity, low pressure drop and a little low separation efficiency in contrast to the returned flow hydrocyclone. This hydrocyclone system has the higher separation property in contrast to the single-level hydrocyclone.

实验结果表明:这种新型入口结构水力旋流器在流体的导旋方面具有独到之处,能很好的形成稳定的流场;同时这种渐缩式入口方式,可将来液的一部分压能逐渐转化为动能,提高了旋流速度,对提高分离效率起到积极作用;采用新型入口的直流式水力旋流器比返流式水力旋流器压降低,处理量大且分离效率略低;组成返流式水力旋流器串联系统相对于单级水力旋流器具有更高的分离性能。

The pilot study shows that the ammonia dissociation/synthesis reaction is inherently free of any potential side reaction problems, and also offers phase separation of products. The carrier of heat store is liquid. and is very interesting for long-term storage as it can be carried out at room temperature with no energy losses, Its theoretics energy storage density reachs 66.5kJ/mol, which outclasses sensible or latent heat storage. In comparison to other thermochemical energy storage, ATCES needs lower temperature in endothermic reaction(only 650℃), but It can produce over 550℃ high temperature stream in exothermic synthesiser. Furthermore, the heat store/recovery can change with adapt to the heat source/load automatically, and offers coninuous(24 h) production of heat, These advantages will see a rapid increase in the adoption of large-scale concentrating solar thermal power generation.

初步研究表明:采用氨基储能体系,储/释过程无副反应,且储能介质能自然发生相分离;储热载体为流体,并可在室温下长期无热损储存,理论储能密度达66.5kJ/mol,远高于显热或相变蓄热;与其它热化学储能相比,吸热反应温度低,仅需650℃,而放热反应可产生500℃以上的高温,能量的储/释流量可随热源/负荷自适应变化,且可全天供热,更适合作为聚光式大规模太阳能热力发电中的能量储存系统。

The numerical result shows that this method can correctly and quickly separate pure compress wave and pure shear wave from hybrid wave field with high precision, good boundary absorption, and the numerical process is stable, so it can be used to guide multi-wave multi-components seismic datum processing, and also point out that the method has difficulty to separate the coupled wave type such as surface wave.

数值模拟结果表明,该方法能够从混合波场中准确地分离出纯纵横波波场,且精度高,边界吸收效果好,计算速度快,计算过程稳定,可用于指导实际多波多分量地震资料处理,同时指出本文算法对相互耦合的波型无法实现准确分离。

In addition to CIP monitoring, the sensor can be used to monitor the conductivity of eluents in chromatographic separations and to detect liquid interfaces.

除CIP监测外,传感器还可被用于监测色谱分离层分离洗提液的传导率,探测液体界面。

And the phenol extraction is a propriety procedure for extraction proteins from aqueous phase. Based on the well-resolved 2-DE patterns, our protein preparation is free of interfering compounds.

使用此方法制备的蛋白质样品进行双向电泳,所得图谱背景清晰,蛋白质点分离效果良好,可有效分离蛋白质点3662个。

Their rotors, three-blade inserts, and all parts contacted with the medium being separated are made of stainless steel or silicon rubber.

分离机转鼓,三叶板等与介质接触部分的所有另部件都采用不锈钢或硅橡胶,除分离血制品外,还可用于一般腐蚀性介质分离。

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推荐网络例句

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。