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Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具,分别以人丁酰胆碱酯酶(1p0i:A)和果蝇乙酰胆碱酯酶(1d×4:A)的蛋白晶体结构为模板,对嗜虫书虱2个AChE的三维结构进行同源建模,并在三维结构中发现了AChE的酶解活性位点,证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少,在GenBank中没有可用作内参基因的序列,因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号:FJ041117),该片段长度为822 bp,编码273个氨基酸残基,同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

By sorting out and summarizing the theories of resource efficiency revolution to make systematical epagogic elaboration on overseas references of 4 factor revolution,10 factor revolution and resource efficiency indexes,and an overview on reference of resource efficiency by domestic scholars and scientific econometric analyses on emergy theories.

在总结前人研究成果的基础上,定义了资源效率革命的概念,提出了资源效率革命具有目标性、根本性、革命性和倍数性的特征;分析了资源效率革命的理论基础是可持续发展理论、循环经济理论,提出循环经济的核心是资源效率革命的观点并进行了深刻剖析。

ConclusionThe method is reliable, stable and repeatable and can be used for the analysis of the volatile constitiuents of Evodia lepta leaves.

结论此方法稳定可靠,重现性好,可用于三叉苦叶挥发性成分的分析。三叉苦叶;挥发油;气相色谱-质谱联用

Based on the wave equation in the acoustic fluid space and the theory of thin-wall structure, the uncoupling error in low frequency range about the nonsymmetrical coupled structure-acoustic analysis equation is studied.

该对称模型特征值的非负性及特征问题的可降阶性也在本文中进行了深入研究,同时指出了在复模态分析中应当注意的问题;(2)由声空间波动方程和薄板理论出发,对非对称声振耦合方程的低频解耦误差问题进行了研究。

By analyzing the factors that have impacts on spatial morphology and landscape pattern,this paper points out that it is the approach to realize the sustainable development of town spatial morphology of the completeness of ecological landscape,the successiveness of the spatial development,the organic nature of spatial elements and the human virtue of spatial morphology.

通过对空间形态和景观格局形成的影响因素分析,提出了小城镇生态景观的完整性、空间生长的连续性、空间要素的有机性与空间形态的人文性是实现小城镇空间形态可持续发展的方法和途径。

Furthermore, three kinds of conflict events,"fulfill positive obligation","violate uncompulsory obligation" and "violate compulsory obligation" were best predictors of parent-child relation after conflict.

研究二以开放式问卷搜集65位大学生的亲子冲突经验,308笔的质性资料经内容分析后显示,以义务观点为基础而建构的冲突事件之分类实存於生活中;另外,将冲突事件分为父母「执行积极义务」、「违反非强制义务」及「违反强制义务」三类最具简约性,且可有效预测冲突后的亲子关系变化。

Based on the practice of nectarine breeding,the systematical inheritance of peel tomenta, flesh color ,pollen fertility and flavor of parents and their hybridization offsprings was analysed.

结合油桃育种实践,对亲本、杂交后代的果皮茸毛、肉色、花粉育性、果实风味等性状进行了系统遗传分析,结果进一步证实了果皮茸毛、肉色、花粉育性3个性状各由1对等位基因控制,其中果皮有茸毛对无茸毛、白肉对黄肉、花粉可育对败育均呈完全显性,且这3对等位基因分别呈独立遗传,无互作效应;选择甜风味的品种作亲本,杂交后代出现甜油桃单株的比例较高。

Plastic Injection the design process, which is designed as part of the craft-oriented establishment : Plastic Parts of the Process Analysis, Plastic Parts of the size and quality of calculation and the injection parameters set; Structural design : Surface choice cavity determination, Cavity the arrangement, gate design, lateral type pulling mechanism design, launch and reattached body identified; Core, Cavity size calculation; Die heating and cooling system computation; Mold closing high set; Injection machine parameters in the verification; The design of such a structure can be used to ensure reliable die.

注塑模中文摘要翻译成英文摘要???帮帮忙~~~救急啊塑料注射的设计过程,其中的设计内容有零件的工艺性编制:塑件的工艺性分析、塑件的体积和质量计算及注射机参数的确定;结构设计:分型面选择、型腔数确定、型腔的排列方式、浇口设计、侧向分型抽芯机构设计、推出及复位机构方式确定;型芯、型腔尺寸计算;模具加热和冷却系统计算;模具闭合高度确定;注射机有关参数的校核;如此设计出的结构可确保模具工作运用可靠。

300 pieces of idioplasm resources of the Tibetan edible cultivation spring naked barley is identified for characteristic of shape, agronomic characters, rust-resisting property and grain thick protein,β-grape polymerization sugar and Vitamin E and so on from the Tibetan different region, which are analysed result in, for one thing, multiplicity of Tibetan spring naked barley is quite rich for the variation of the awn, leaves and the grain color, and multiple property are highest; Secondly, it has some fine hereditary characters of early maturation, big spike, many grains, many arrises and the strong anti-rust quality; Thirdly, genetic potential which is used possibly is enormous for characteristic of dense spike, exiguous spike, naked grain , the hook awn, short stem, the anti-rust and so on ; Fourthly, thick protein,β-grape polymerization sugar and Vitamin E of grains are rather difference to environmental condition of the various areas.

从西藏各地征集的300余份食用栽培春青稞种质资源,从形态特征、农艺性状、抗锈性和籽粒粗蛋白、β-葡聚糖含量、维生素E等特征特性鉴定、评价。分析结果认为:(1)西藏春青稞多样性极为丰富,如芒、稃和籽粒颜色,具有变异最为丰富,多样性最高的特点;(2)具有早熟、大穗、多粒、棱型多,以及抗锈性强等和耐各种不良环境因素的优良遗传性状;(3)具有密穗、稀穗、裸粒、钩芒、矮生、抗锈等可利用基因潜力巨大等特色;(4)籽粒粗蛋白、β-葡聚糖、维生素E等成份因各地区环境条件不同差异较大。

It is concluded that the positive rate of platelet antibody screening is very high in patients with hematologic malignancies, the coincidence rate of platelet antibody crossmatch in 249 blood samples is between 40% and 48%, and the effeciency of using crossmatched platelets in clinic is enhanced significantly.

微柱凝胶免疫分析技术在血小板抗体筛选中的应用大部分免疫性血小板减少性紫癜患者血小板膜上可测出血小板相关抗体,包括IgG、PA IgM、PA IgA,其中一项出现阳性,即为血小板抗体检测阳性,而且其量往往与外周血的血小板数量呈负相关,因此检测这种血小板相关抗体有助于免疫性血小板减少性紫癜的诊断[1]。

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推荐网络例句

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。