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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I,并成功地在原核细胞E.coli进行了重组表达,筛选出稳定表达的工程菌株DH5α,摸索了稳定表达的条件,摸索了包涵体的洗涤、变性和复性条件,摸索出了复性蛋白Src I的纯化工艺,纯化过程简单,经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化,就可获得了高纯度的重组蛋白样品(纯度达99%以上),该纯化方法适合重组蛋白的大量纯化,为Src I的性质、结构和功能研究奠定了良好的基础。

Triplication ECC provides a more reliable mechanism to advanced technologies. Moreover, in view of lower latency of decoder, it has rapid correction ability to reduce the physical transfer unit size of switch fabrics by self-corrected in bit level.

随著更先进制程,三重错误更正码将提供更可靠的更正机制,此外由於此方法可迅速编解码,使之能有效地降低晶片网路交换结构中的位元数。

Even in the ordinary case of the exchange of promise for promise,§ 78 makes it clear that voidable and unenforceable promises may be consideration.

即使在允诺交换允诺的通常情况下,第78条也清楚地表明:可撤销的和不可执行的允诺也可能成为约因。

Results:(1) Dobutamine increased 〓 exchange currents with a concentration-dependent manner in ventricular myocytes of normal and hypertrophied rats.

结果:(1)多巴酚丁胺可剂量依赖性地增加正常大鼠和高血压肥厚大鼠心室肌细胞的〓交换电流。

The next year I did better still, for I spaded up all the land which I required, about a third of an acre, and I learned from the experience of both years, not being in the least awed by many celebrated works on husbandry, Arthur Young among the rest, that if one would live simply and eat only the crop which he raised, and raise no more than he ate, and not exchange it for an insufficient quantity of more luxurious and expensive things, he would need to cultivate only a few rods of ground, and that it would be cheaper to spade up that than to use oxen to plow it, and to select a fresh spot from time to time than to manure the old, and he could do all his necessary farm work as it were with his left hand at odd hours in the summer; and thus he would not be tied to an ox, or horse, or cow, or pig, as at present.

扬的著作在内。我发现一个人如果要简单地生活,只吃他自己收获的粮食,而且并不耕种得超过他的需要,也不无餍足地交换更奢侈、更昂贵的物品,那末他只要耕几平方杆的地就够了:用铲子比用牛耕又便宜得多;每次可更换一块新地,以免给旧地不断地施肥,而一切农场上的必要劳动,只要他夏天有空闲的时候略略做一做就够了;这样他就不会像日前的人们那样去和一头牛,或马,或母牛,或猪猡,捆绑在一起。

The application results from fluorination showed microwave heating could accelerate chemical reactions markedly. The reaction time could be shortened 50% at least than that of conventional heating. The relationship between fluorinating agents\' structure and their activity was studied systematically. In addition, an anhydrous KF with higher activity was prepared by using expansion effect of microwave heating. As we known, solvents were very important to reaction. The comprehensive study showed some less polar aromatic solvents could be used as dipolar solvents. In addition, they had better effect than dipolar solvents in some occasions, such as fluorination of chlorobenzaldehydes and chlorinated diphenyl ketones etc. The primary applications of ionic liquids were also studied which indicated reactions with ionic liquids as solvents were more efficient and simple.

研究表明,微波是一种节能高效的加热方式,在微波作用下的卤素交换氟化反应具有反应速度快、转化率高、选择性好的优点,其反应时间可较常规加热缩短50%以上;同时也系统地研究了氟化剂种类及制备方式对其反应活性的影响,并利用微波加热的膨化作用制备出了一种活性较高的KF,其活性与喷雾干燥KF相差不大;溶剂对反应具有重要的作用,在此系统地研究了强极性非质子溶剂和中等极性非质子溶剂的应用情况,发现一些中等极性的非质子溶剂如硝基芳烃和氯代芳烃类溶剂在氯代苯甲醛类化合物的氟化反应中具有比强极性非质子溶剂更好的使用效果。

The results showed that there were significant differences in numbers of stolons, length of stolons, dry weight of genets, dry weight of stolons and dry weight of ramets of L. brachystachya in different plots with different altitudes (P.01). The numbers of stolons of L.

在一定时间内,克隆植物相互连接的克隆分株可生长在不同环境条件的斑块中,并彼此进行物质交换、实现克隆整合,从而最大可能地利用异质环境 [5]。

Available in a wide range of firmness levels, it is simple to build a mattress that caters to an individuals needs; his and her sided beds are easy to construct; toppers can fine tune the feel to fit anybody's preference, and can easily be exchanged to ensure you feel as comfortable as possible when it matters most.

可提供范围广泛的坚定性水平,这是简单的建立一个床垫,满足了个人的需要;他和她的片面床很容易构造; toppers可以微调的感觉适合任何人的喜好,并可以方便地进行交换,以确保您觉得的尽可能舒适,最重要的当。

Eachsession is uniquely identified in a transmission header by a pair of network addresses, identifying the origin and destination NAUs of any transmissions exch anged during the session.

在传输报头中,通过一对网络地址,唯一明确地标识著一条话路,这对网络地址还标明:在两个NAU进行通话期间,对任何要交换的传输信息﹝报文,信元﹞而言,谁是发信的网络可编址单元,谁是收信的网络可编址单元。

Eachsession is uniquely identified in a transmission header by a pair of network addresses, identifying the origin and destination NAUs of any transmissions exch anged during the session.

在传输报头中,通过一对网络地址,唯一明确地标识着一条话路,这对网络地址还标明:在两个NAU进行通话期间,对任何要交换的传输信息〔报文,信元〕而言,谁是发信的网络可编址单元,谁是收信的网络可编址单元。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。