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Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

A common weed, Peterson's Guide recommends our eating them when they are very young, as they soon become stringy and bitter.

一种常见的杂草, Peterson的《指南》中提醒我们要吃嫩的,因为随着这种草的成熟会变得越来越粘越来越苦。

In the path of growth, successive tests so that we become mature and stable.

在成长的道路上,接连不断的考验使我们变的成熟、稳重。

In the path of growth, We expereince successive tests so that we become mature and stable.

在成长的道路上,接连不断的考验使我们变的成熟、稳重。

Artistic feeling explains cosmopolitan life lithe and gracefully and flowery, maturity and lovely, take among them hold so that had better become money of shoe of the head that belong to a circle, the different adornment on toe cap lets shoe and you at the same time perfect change personally.

艺术的感觉把大都会的生活诠释得曼妙而绚丽、成熟与可爱,其中拿捏得最好当属圆头鞋款,鞋头上的不同装饰让鞋子和你同时完美变身。

OVC has an important role in the physiological and pathophysiological process of liver cell development, growth, proliferation, and carcinomatous change.

肝脏严重损伤时,卵圆细胞受自身状态和微环境的调节,分化为成熟肝细胞和胆管细胞,但其分化方向并不是固定不变,有研究表明,卵圆细胞在特定的自身状态和微环境的调节下,可以分化为肝癌细胞。

And as manufacturing became more technologically stable and product lines matured during the postwar boom, suburban growth dovetailed nicely with the pattern of industrial growth.

随着战后繁荣带来的制造业在技术上变得更加稳定,产品线更为成熟,郊区的增速和产业的增速密切配合。

Fruit drupaceous, of separating mericarps or ± completely syncarpous, drying brown to black or rarely pale gray-brown when ripe; abortive carpels, if any, persistent; outer pericarp ± fleshy; endocarp bony next to locule, otherwise woody, in mericarps developed from 2-ovuled carpels forming a median longitudinal partial partition within locule.

果核果状,有分离的分果瓣或者多少完全的合心皮,成熟后变干,棕色或黑色,很少灰棕色;败育心皮,如果有的话,宿存;外部果皮多少肉质;内果皮骨质或木质,在分果瓣里内果皮由2胚珠的心皮在每室内形成中间的分隔。

Only in adulthood the inability of these neurons to receie GDNF due to the missing receptor became eident and the older the animals grew, the more nere cells died.

仅仅在成熟期间随着这些神经元因失去受体变的明显并且动物长的越老,细胞的死亡越多从而无力接受GDNF 。

When a lack of folic acid, some amino acids in each variable delay so that the formation of red blood cells in the process of nucleic acid synthesis of obstacles, red blood cell development and maturation blocked, causing giant erythroblastic anemia, folic acid can be used at this time for treatment, so folic acid also called the anti-anemia vitamin.

当叶酸缺乏时,某些氨基酸的互变受阻,使红血球形成过程中的核酸合成障碍,红血球的发育和成熟受阻,引起巨幼红细胞性贫血,这时可以用叶酸进行治疗,故叶酸也叫抗贫血维生素。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。