变性蛋白

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

Based on these observations, we propose that the periplasmic proteins have been evolved to resist the formation of aggregates when subjected to various denaturing conditions and molecular chaperones may thus not be needed in periplasm.

根据这些发现，我们提出假说：膜间质蛋白在恶劣环境条件的选择压力下进化出对各种变性条件所诱导聚集的抗性，而不需要分子伴侣蛋白的有效保护。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

The tripeptide it contains, under the penetration by transdermal enhancer, can rapidly combine with reducing sugar to effectively reduce the bond between collagen caused by the combination of reducing sugar and collagen, avoid protein denaturation which leads to impoverished skin, and remove the fine lines and wrinkles of skin.

添加三胜肽在透皮促进剂的渗透下迅速与还原糖结合，有效地减少还原糖与胶原蛋白的结合所造成胶原蛋白相互交结，避免蛋白质变性而使皮肤失去弹性，并可以驰缓组织，抚平皮肤上既有的细纹与皱纹。

Both fusion proteins were purified under denaturing conditions which favor the transduction of proteins across the plasma membrane.

这两种蛋白皆是在变性的条件下纯化，这是为了利於蛋白穿透细胞膜。

A small amount of soluble Trx-IPSE was obtained by dialyzing the fusion protein in a large volume of PBS.

用大量PBS对变性融合蛋白进行透析，获得部分复性的可溶性Trx-IPSE融合蛋白。

This paper studied the effect of urea modification on adhesion strength and structure change of soy protein isolates.

研究了尿素变性对大豆分离蛋白粘接强度及对蛋白分子结构的影响。

Dystrophin plays a key role in building and repairing muscle; without it, muscles deteriorate and lose function.

肌营养不良蛋白在肌肉的生长和修复中扮演了重要的角色，如果没有这种蛋白，肌肉就会变性和失去功能。

The results are as follows: the DDM detergent dissolves the Cyt b6f better thanβ-OG detergent does; DDM can keep the Cyt b6f in native state, under which the hemes and Chl a experience specific environment, while theβ-OG make the complex in denatured state in some sort, under which the catalyzed electron transfer activity of the complex

结果表明，DDM去垢剂能使Cyt b6f处于较好的分散体系中，其中血红素和Chl a分子处于特定的蛋白环境中，不会导致Cyt b6f变性；而β-OG去垢剂会使Cyt b6f产生聚合现象，其中的血红素和Chl a与蛋白环境的相互作用减弱，和DDM相比其电子传递活性显著降低，Chl a单线激发态寿命延长，Chl a更容易被光

Coli. The protein was expressed in a soluble form and as a fusion protein. There was a 6 histidines tag at its amino acid terminus. It was purified by immobilized metal ion affinity chromatography under native conditions.

所表达的蛋白是一种可溶性的融合蛋白，其氨基端带有6个组氨酸的标签，因此可以用固定化金属离子亲和层析法在非变性条件下进行纯化。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。