变性蛋白

Firstly,The crosslink of sodium caseinate and whey protein isolateat three different conditions using transglutaminasewas studied.The extent of polymerization was detemined by SDS-PAGE、electron microscope and infrared spectrometer associated with gel image analysis.

通过对各组分蛋白相对光密度值的变化分析可知：在本实验所选条件下，在80℃预热15min后并再加入一定量还原剂(20mmoL/LDTT)变性条件下，酪蛋白和乳清蛋白之间可以交联；为进一步验证上述结论，通过中红外光谱仪和电子显微镜进一步分析交联前后蛋白质的二级结构的变化和交联前后蛋白质空间结构上的差异，结果分析均与上述结论相吻合。

However, the final temperature of processed meats could not sufficient to denature the main components of soy protein (7S and 11S), thereby limiting its interaction with muscle proteins.

然而当其应用到肉品加工过程中，大豆分离蛋白的主要成分(7S和11S)并不能表现出理想的结构变化，抑制肉品中的蛋白变性，导致大豆分离蛋白在应用上的局限性。

She and her colleagues used the detergent SDS, which is usually used as denaturant to degrade protein, to activate hemocyanin. At certain high concentrations, instead of destroyomg the complex, it turns hemocyanin into an enzymatically active phenoloxidase.

她和她的同事用一种经常被用作蛋白质变性剂的十二烷基磺酸钠来活化血蓝蛋白，它在一定的高浓度下，增强血蓝蛋白酚氧化酶的活性而不会破坏血蓝蛋白复合物。

Metamorphic protein element can condense into a skin, form very firm film to surround the air of interfuse inclose, in the meantime, as a result of the action of exterior tension, make albumen bubble contractive become globose, add albumen colloid to have viscosity and affiliation flour raw material to adhere to it is all round albumen bubble, make bubble becomes very stable, can carry the gas of interfuse, in heating process, the gas inside bubble be heated expands, make goods loose and poriferous and have certain flexibility and tenacity.

变性的蛋白质分子可以凝聚成一层皮，形成十分牢固的薄膜将混入的空气包围起来，同时，由于表面张力的作用，使得蛋白泡沫收缩变成球形，加上蛋白胶体具有黏度和加入的面粉原料附着在蛋白泡沫四周，使泡沫变得很稳定，能保持住混入的气体，加热的过程中，泡沫内的气体又受热膨胀，使制品疏松多孔并具有一定的弹性和韧性。

We also got STM images of denatured fibroin under stress or by methanolin molecular level. We found the conformation of silk fibroin is changed from α-helixand random coil structures to β-strand and loosed α-helix formation. We employed the STM to explore the structure and morphology of silk fibroinin the middle silk gland of mature silkworm.

我们在分子水平上对在冰冻和甲醇处理下的丝心蛋白的变性情况进行了STM研究，我们发现丝心蛋白的构象由α-螺旋结构和无规线团的结构向β-伸展链和松散折叠α-螺旋结构转变我们还用STM对熟蚕的中部丝腺体中的丝心蛋白的结构形态进行了研究。

Western-blot analysis and indirect immunofluorescence assay showed that the gp90 protein expressed successfully was 45ku in molecular weight,possessed good reactionogenicity and could be recognized by mouse hyperimmune sera against the gp90 protein under natural or denatured state.

抗gp90蛋白小鼠超免疫血清介导的Western-blot分析和间接免疫荧光试验结果显示，gp90蛋白被正确表达，分子质量约为45ku；gp90蛋白在变性和自然状态下均能够被识别，具有良好的生物活性。

A potential approach to this problem is to make use of a natural defense mechanism called " the stress response," which is characterized by a transient down - regulated synthesis of nonvital cellular proteins and increased production of endogenous protective factors known as heat shock proteins that act to allow proper refolding of proteins denatured by the incipient injury.

应激反应的特征是一过性下调细胞非必需蛋白的合成及增加被称为热休克蛋白的内源性保护因子的合成。热休克蛋白作用是促使损伤导致变性的蛋白适当地再折叠。HSP家族中HSP70被认为在细胞保护中发挥重要作用。

The results showed that Tang-an-kang could respond symptomatically, lower blood-sugar, diminish urinary protein, regulate endogenous creatinine clearance rate and lipid metabolism etc. It was suggested that Tang-an-kang had a good action on improvement lipid metabolism, diminishing urinary protein and renal function protection.

结果表明：糖安康治疗组在改善DN患者临床症状，降低血糖、糖基化血红蛋白及血肌酐，减少尿蛋白及尿白蛋白，调节内生肌酐清除率，降低血与尿β2微球蛋白，调节脂代谢，改善血液流变性等方面，疗效均优于单用西药的对照组，提示糖安康有良好改善糖脂代谢、减少尿蛋白、保护肾功能的作用。

Effects of microbial transglutaminase on the tensile properties of plastic made from soy protein isolates are studied. The results show that the tensile strength and Young modulus of the protein plastic are increased from 9.2MPa and 265.4MPa of the control, which is not treated with TG, to 14.7MPa and 390.1Mpa respective, which is treated with TG at 50℃ for 60min. High molecular weight protein conjugates are formed with TG treatment, and they are responsible for the enhanced tensile properties, denaturalizing temperature and enthalpy values.

研究了微生物谷氨酰氨转胺酶对大豆分离蛋白塑料拉伸性能的影响，发现在50℃时转氨酶对大豆分离蛋白催化聚合反应60 min，形成的高分子聚合物使大豆分离蛋白的抗拉强度和杨氏模量分别由未经处理时的9.2 MPa和265.4 MPa增加到14.7 MPa和390.1 MPa，同时增加了蛋白质的变性温度和热焓值。

METHODS: The rhBMP-2,expressed in Escherichia coli, was washed by Triton X- 100 and further purified by DEAE chromatography.The inclusion bodies were resolved in 8 mol/L urea, and were refolded and dimerized in the redox systems (reduced and oxidized glutathione). Finally, a one - step purification procedure based on the heparin affinity chromatography was implemented. The biological activity of purified rhBMP - 2 were tested by induction of the alkaline phosphatase activity in C2C12 cells.

在大肠杆菌中通过温度诱导表达重组人骨形成蛋白-2经过Triton X-100清洗之后，又通过DEAE离子交换层析纯化包涵体，包涵体在8 mol/L尿素变性溶解，在氧化-还原(还原型和氧化型谷胱甘肽)复性系统中，通过简单的稀释复性，通过肝素亲和层析一步纯化法纯化重组人骨形成蛋白-2，最后通过诱导C2C12细胞产生碱性磷酸酶检测重组人骨形成蛋白-2活性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。