变性蛋白

The resolution and renaturation efficiency of lysozyme were found to be independent of the flow rate and gradient time when the volume of mobile phase was kept a constant.

在计量置换模型的基础上首次提出了流动相用量估算公式，用实验对其进行了检验，并得出在保持流动相用量不变的条件下，改变流速和梯度时间对分离度和变性蛋白的复性效率影响不大的结论。

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响，认为其在沉降大分子物质和变性蛋白的同时，也必然会使目标蛋白有所损失。

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响，认为其在沉降分子物质和变性蛋白的同时，也必然会使目标蛋白有所损失。

Meantime, we also discuss the electrochemical investigations of the denatured proteins, towhich was just attached importance in 1990s.

同时，对九十年代刚刚引起重视的变性蛋白的电化学研究进行了评述。

Laminaria japonica and Undaria pinnatifida, representative species of brown algae, inhabit subtidal zone; Ulva pertusa and Enteromorpha prolifera, representative species of green algae, inhabit intertidal zone.

热休克蛋白70是热休克蛋白家族中重要的成员，参与新生蛋白的折叠、转运、重折叠变性蛋白、协助降解变性蛋白和抗逆环境胁迫等功能。

And finally, a suggested refolding mechanism of reduced-denatured egg white lysozymes in urea solution was presented.

最后，给出了一个建议的还原变性蛋白溶菌酶分子在脲溶液中的折叠机理。

The aggregation interaction between reduced-denatured egg white lysozymes during refolding procedure in urea solution was studied by means of reducing and non-reducing protein electrophoreses.

通过还原和非还原蛋白电泳，研究了还原变性蛋白溶菌酶在脲溶液中重折叠过程的集聚相互作用。

The refolding of reduced and non-reducing egg white lysozymes in a urea solution was studied by a "phase diagram" method of fluorescence.

用荧光&相图&法研究了还原和非还原脲变性蛋白溶菌酶的重折叠。

Results of non-reducing sodium dodecyl sulphate polyacrylamide gel electrophoresis of the supernatant and aggregate precipitate formed in refolding process show that except being refolded to native egg white lysozymes, the reduced-denatured lysozymes can also form the aggregates with molecular weights being separately about 30.0 and 35.0 kD, while the reducing SDS-PAGE and the refolding results in the presence of sodium dodecyl sulphate show that these aggregates are formed chiefly through the misconnection of disulfide bonds between the reduced-denatured lysozymes, and the aggregate precipitates are formed through the non-covalent interactions between the aggregates with molecular weight being about 30.0 kD.

复性过程形成的上清和集聚体沉淀的非还原电泳结果表明，除了能复性成天然态的溶菌酶分子外，还原变性蛋白溶菌酶同时还能形成分子量分别约为30.0KD和35.0KD的蛋白溶菌酶分子集聚体；而它们的还原电泳和在SDS存在时的复性结果表明，这些集聚体主要是通过还原变性蛋白溶菌酶分子间的二硫键错配而形成，而集聚体沉淀则是通过分子量约为30.0KD的集聚体分子之间的非共价相互作用而形成。

We found, on the one hand, urea can cause cytochrome cboth on the electrode surface and in the solution to be denatured and then the denaturedprotein lends to move the formal potential of the native protein in the bulk solution to amore negative value; and on the other hand, urea can also cause the formal potential ofnative protein in the bulk solution to shift positively with decreasing the degrees ofdissociation of electrolytes and polarity of the urea-water solvent.

我们发现脲能引起电极表面和溶液中的细胞色素C的变性，变性蛋白质能导致本体溶液中天然蛋白质的标准式电位的负移；另一方面，通过降低电解质的离解度和脲-水溶剂的极性，脲能使本体溶液中天然蛋白质的标准式电位正移。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。