变性反应

Chain reactions are triggered which lead to retinal pigment epithelium degeneration,Bruch's membrane breakdown, CNV formation, AMD and blindness in the end.

经过一系列反应，最终导致视网膜色素上皮细胞变性、Bruch膜断裂、脉络膜新生血管形成、老年黄斑变性、失明。

Optimal condition of denaturalization is given by: temperature, 45℃ treatment time, 15min; concentration of NaSO3, 0.15%.

变性处理的最佳条件为Na2SO3浓度0.15%，温度45℃，变性反应时间15min。

In most denaturation experiments, the high concentrations of denaturants may greatly affect the kinetic behavior of the system to preclude the use of the kinetic parameters determined in the absence of denaturant.

在大多数蛋白质变性实验中，高浓度的变性剂可能对酶反应体系的动力学行为有很大影响，因此在没有变性剂存在时测定的动力学参数不再适用。

METHODS: The procedure conditions of PRC: 40 cycles of denaturation temperature from 88 ℃ to 97 ℃ for 5 s and 40 s respectively, and the temperatures of anneal and extension was 60 ℃ for 40 s.

聚合酶链反应运行程序条件：变性温度分别为88~97 ℃，变性时间分别为5 s和40 s，退火和延伸60 ℃，40 s，循环数40。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

From probability consideration, the curing theory of A〓+A〓-type and A〓+ B〓-type polymerization involving initiation stepis proposed. The sol-gel distribution equations which can describethe behaviour of the conversions in the curing process are givenand the gelation condition, the number fractional distribution andits invariant property are obtained.

针对含引发机制的〓型和〓型缩聚反应体系的固化问题，利用概率分析的方法进行了系统的理论研究，给出了这两类反应体系的溶胶凝胶分配方程，从而揭示了体系在固化过程中的变化规律，得到了这两类反应体系的凝胶化条件、数量分布函数及其不变性。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应，a波潜伏期缩短o叼。05）；术后2周的暗适应视杆反应，a波潜伏期延长o功刀5）：术后1周的暗适应最大反应，b波潜伏期缩短o功刀5卜＊CS大鼠术后4周的暗适应视杆反应和明适应反应，a波振幅增大o功刀匀；术后4周的暗适应视杆反应，a波潜伏期缩短o＜0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡，导致FERG的a、b波振幅明显降低；视网膜下腔仍存在感光细胞外节盘膜碎屑，外丛状层缺乏感光细胞的突触末端，同时Muler细胞出现反应性胶质纤维增生。

The influence of thixotropic reactive resin—Ac resin the automobile coating have been studied.

研究了触变性反应型树脂对汽车涂料性能的影响。

The effect factors of solid content, alkali, reaction temperature, reaction time, cross-linking agent, starch, polyvinyl alcohol, termination reaction point, resistivity against water, alkali washfastness on the performance of the adhesive for labeling beer bottle were investigated.

采用乾酪素、聚乙烯醇、酸变性淀粉为主要原料，制备改性酪蛋白啤酒瓶标签胶，讨论了固含量、碱、反应温度、反应时间、交联剂、酸变性淀粉、聚乙烯醇、终点判断、耐水性、碱洗净性等因素对酪蛋白商标胶的制备与性能的影响。

This article summarises the chemical reactions in milk during heat treatment, analyzes the properties of several indicators for heat damage , such as heat-sensitive enzymes, denatured proteins, the products of Mailliard Reaction and isomerised lactose.

本文分析了牛乳在热加工过程中的化学变化，介绍了几种牛乳热损伤监控标识物(如热敏性酶类，蛋白质变性产物，美拉德反应产物和乳糖异构化产物)的特点，并详细论述了已有的牛乳热损伤监控技术：对变性β-乳球蛋白含量的监控，对糠氨酸和乳果糖含量综合水平的监控，对羟甲基糠醛含量的监控，以及以变性蛋白质(以色氨酸的荧光强度计，FTry)和美拉德反应荧光产物的聚集量为基础，评估牛乳热加工损伤程度的FAST方法。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。