变性作用

The result showed that the temperature of denature for the formeris 106.67°C, while that for the latter is 66.84°C. These results showed that theoligosaccharide chain of SPWSG played a role of strengthening resistance todenaturation.

采用DSC对甘薯水溶性糖蛋白的热力学性质进行了研究，结果表明甘薯水溶性糖蛋白的热变性温度为106.67°C，而去糖基后的热变性温度为66.84°C，说明糖蛋白上的寡糖链对其有较好的稳定作用，具有增强糖蛋白抗变性的功能。

The equilibrium association constant K of DNR with fish sperm DNA is 1.98×105 mol/L and that of DNR with denaturated DNA is 2.29×104 L/mol.

研究发现嵌入作用是柔红霉素和天然DNA之间的主要作用方式；并且柔红霉素和天然DNA之间的作用要强于和热变性单链DNA之间的作用。

Objective To examine the role of apoptosis in photoreceptor cell death in age related macular degeneration.

老年黄斑变性中感光细胞的凋亡徐格致李维英曹安民目的探讨细胞凋亡在老年黄斑变性感光细胞死亡中的作用。

Objective To study the expression of tumor necrosis factor-α, intercellular adhesion molecule-1(ICAM-1) and S-100 in the process of pigmentary degeneration, and its significance.

目的 观察视网膜色素变性过程中，视网膜外节层内组织细胞肿瘤坏死因子α、神经组织蛋白S-100、细胞间黏附分子－1(ICAM-1)的表达情况，探讨其在视网膜色素变性过程中的作用及意义。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

PartⅡ:Effect of bicyclol on hepatocyte apoptosis in alcohol-intoxicated mice and related mechanismsThree consecutive intragastric administration of alcohol(6g/kg)in 12h interval resulted in an obvious liver injury and inflammation in mice,as indicated by the elevation of serum alanine transaminase(2.2 fold of control),increased hepatic COX-2 protein expression(2.2 fold of control),as well as liver pathological changes characterized by myelonic degeneration,vacuolar degeneration and inflammatory infiltration.

双环醇对酒精中毒小鼠肝脏细胞凋亡的保护作用及机制研究小鼠连续三次酒精(6g/kg)灌胃后可导致明显肝损伤，变现为血清ALT水平升高(为对照组的2.2倍)，肝脏炎症相关蛋白环氧合酶-2(cyclooxygenase-2，COX-2)表达增加(为对照组的2.2倍)，肝组织出现髓样变性、小空泡变性和炎性细胞浸润。

This paper reviewed the effect of some major neurotrophic factors on diabetic neuropathy and their application in clinical treatment.

本文对一些主要的神经营养因子对糖尿病性神经病理变性及再生作用，以及神经营养因子在临床糖尿病性神经变性的应用研究作－综述。

Objective The purpose of this study was to investigate whether subretinal transplantation of Müller cells with neural stem cell properties can rescue photoreceptor degeneration in RCS rat.

目的 以RCS视网膜色素变性大鼠为模型，研究经诱导产生神经干细胞特性的Müller细胞视网膜下腔移植对光感受器变性的保护作用。

Based on the theories of rheology, Non-Newtonian Fluid Mechanics, petroleum chemistry, crystallography and pipeline technology etc. and sampling Huabei crudes, experimental indexes and programmes are drew out, integrating the oil properties and parameter analysis; in terms of the macro analysis of continuum mechanics, substantial experiments are conducted with HAAKE RS600 rotational rheometer and the rheological equation as well as the viscosity-temperature equation are further developed with tested rhrogramme and viscosity-temperature curve, respectively. Beased on the researches on thixotropic mechanism and thixotropy fluid property, hysteresis loop curve, structure failure and viscosity build-up curve as well as shear stress decrement curve for one type oil are first present in this paper. According to the theoretical researches and testing results, a novel thixotropy equation with τ=τ_∞e~ pattern, which is of hi-accuracy, few parameter and simplicity, is developed. Considering the influence of various parameters on rheological behaviour, depressant adding condition is optimized and experimental evaluation on BEM-5P is conducted, which further unrip its mechanism on the Huabei crude. A corresponding viable pipe transportion programmel is also recommended.

本文基于原油流变学、非牛顿流体力学、石油化学、结晶学、原油管输工艺学等理论，以华北原油为研究对象，结合原油物性及实验影响因素分析，制定出实验研究的评价指标及实验方案；从连续介质力学宏观分析的角度，利用HAAKE RS600高端旋转流变仪开展了大量的室内实验，并运用微观研究方法，测定了油样的流变曲线和粘温曲线，建立了流变方程和粘温方程；通过对触变性机理和触变性流体特征的研究，首次对华北原油测定出滞回环曲线、结构破坏与恢复粘时曲线、剪切应力裂降过程曲线，并在理论研究和实验结果的基础之上，建立了能定量描述含蜡原油剪切应力裂降过程的新型三参数触变模式τ=τ_∞e~b/(t+c，该模型具有精度高、参数少、实验简便且适用性强的特点；通过对含蜡原油流变性的影响因素理论研究，开展了加剂条件优选及BEM-5P降凝剂室内评价实验，进一步揭示了降凝剂对华北原油作用机理，在此基础之上，提出了华北原油管输的可行性方案。

Based on the theories of rheology, Non-Newtonian Fluid Mechanics, petroleum chemistry, crystallography and pipeline technology etc. and sampling Huabei crudes, experimental indexes and programmes are drew out, integrating the oil properties and parameter analysis; in terms of the macro analysis of continuum mechanics, substantial experiments are conducted with HAAKE RS600 rotational rheometer and the rheological equation as well as the viscosity-temperature equation are further developed with tested rhrogramme and viscosity-temperature curve, respectively. Beased on the researches on thixotropic mechanism and thixotropy fluid property, hysteresis loop curve, structure failure and viscosity build-up curve as well as shear stress decrement curve for one type oil are first present in this paper. According to the theoretical researches and testing results, a novel thixotropy equationwith τ=τ_∞e~ pattern, which is of hi-accuracy, few parameter and simplicity, is developed.Considering the influence of various parameters on rheological behaviour, depressant adding condition is optimized and experimental evaluation on BEM-5P is conducted, which further unrip its mechanism on the Huabei crude. A corresponding viable pipe transportion programmel is also recommended.

本文基于原油流变学、非牛顿流体力学、石油化学、结晶学、原油管输工艺学等理论，以华北原油为研究对象，结合原油物性及实验影响因素分析，制定出实验研究的评价指标及实验方案；从连续介质力学宏观分析的角度，利用HAAKE RS600高端旋转流变仪开展了大量的室内实验，并运用微观研究方法，测定了油样的流变曲线和粘温曲线，建立了流变方程和粘温方程；通过对触变性机理和触变性流体特征的研究，首次对华北原油测定出滞回环曲线、结构破坏与恢复粘时曲线、剪切应力裂降过程曲线，并在理论研究和实验结果的基础之上，建立了能定量描述含蜡原油剪切应力裂降过程的新型三参数触变模式τ=τ_∞e~b/(t+c，该模型具有精度高、参数少、实验简便且适用性强的特点；通过对含蜡原油流变性的影响因素理论研究，开展了加剂条件优选及BEM-5P降凝剂室内评价实验，进一步揭示了降凝剂对华北原油作用机理，在此基础之上，提出了华北原油管输的可行性方案。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失