变性作用

A primer was designed based on the conserved regions of other MBL plants agglutinin through homology alignment.

盐酸胍较脲可产生更强的变性作用。

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果：(1)在无血清条件下单独培养的DRG，背根神经节的轴突数目众多，外形纤细弯曲，不成束，并且施万细胞和成纤维细胞稀少，所以可以排除两者对轴突生长的影响，为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件；(2)在无血清条件下DRG和变性坐骨神经段联合培养，①先单独培养DRG，4天后待神经元轴突长出，再与坐骨神经段联合培养，观察到神经元的轴突数目减少，外形挺直，部分轴突之间相互粘附成束；②变性坐骨神经段和DRG同时联合培养，神经元的轴突数目明显减少，外形粗壮，轴突之间相互粘附成束；(3)有血清条件下单独培养DRG，轴突数目较多，外形挺直，长短不一，部分神经元的轴突之间相互粘附，施万细胞和成纤维细胞数目众多，观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

When pH is below 1 0, DNA denaturalization of pEGFPC3 was prominent and there was hardly protection by β-cyclodextrin.

结果 pH 1 0的酸液对pEGFPC3质粒DNA的变性作用最强，pH 1 5～ 2 5的酸液对其有部分变性作用，pH 1 5～ 2 。5范围内β-环状糊精对质粒DNA具有较为明显的保护作用，对于pH 1 0的情况仍不足提供相应的保护。

Values of hydration number of amino acids have been calculated at different temperature and concentration of denaturant. It is found that they decrease with increasing temperature and concentration of denaturant. Denaturant and increasing temperature have cooperative dehydration effect on amino acids.

计算了不同温度和变性剂浓度下氨基酸在乙酸钠、丁酸钠、己酸钠、辛酸钠和盐酸胍水溶液中的理论水化数，发现它们随变性剂浓度的增加和温度的升高而减小，说明变性剂和升温对氨基酸有协同脱水作用。

This change is considered to be due to denaturation of the enzyme.

据认为这种变化是由于酶的变性作用造成的。

It was found that the denaturation of hemoglobin might not occur at the surface of the silver electrode.

实验表明，血红蛋白在银电极表面无变性作用发生。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

Amylin plays a critical role in islet amyloidosis and in the deelopment of β-cell dysfunction in patients with diabetes; howeer, the inolement of amylin in renal amyloidosis has not been studied.

胰淀素在糖尿病患者的胰岛淀粉样变性及β-细胞机能异常过程中起关键作用；然而尚未研究过胰淀素在肾脏淀粉样变性中的作用。

Simultaneously, the total extractible myoglobin decreased and the proportion of metmyoglobin increased significantly when the pressure above 400MPa. Beef discoloration after pressure processing may result from oxidation of ferrous myoglobin to ferric metmyoglobin, globin denaturation and structural changes. At 40℃, the changes of beef color was similar to that of room temperature's. When the temperature rise to 60℃, most of muscle proteins denatured, a small quantity of native myoglobin could not affect the color of beef significantly. Therefore, protein denaturation was attributed to the main cause of discoloration in beef muscle after pressurization at 60℃.

压力处理导致色泽变化的直接原因可能是由于二价铁的肌红蛋白氧化成三价铁的高铁肌红蛋白、球蛋白的变性及结构的变化所致。40℃时的压力处理，色泽的变化规律与室温下相似，只是色泽变化的程度更为剧烈，蛋白质变性在色泽变化中起到的作用逐渐加强。60℃下的压力处理，绝大部分蛋白质发生变性，少量未变性的肌红蛋白对色泽不会产生明显的影响，此温度的压力处理，蛋白质变性是造成肌肉失去红色的主要原因。

It is able to smelt S, S-Ca, S-RE and S-Ca-RE system free-cutting steel in acid furnace.

本文研究了Ca、RE对硫系易切削钢中易切削相的变性作用。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失