变弱了的

In an integrated space, different parts are endowed with different nature, and there is no boundary in their connections. In those areas where the functions become vague, rules and order would break loose, backing away from the industrial nature in the modernism city to get close to the natural rule, or rather, the weak rules.

在一个整体的空间中，不同部分被赋予了不同的属性，而他们之间的联系是没有明显边界的，在这些功能性模糊的区域里，规则和秩序变得很松散，远离现代主义城市体现出的明显的工业属性，而更接近于自然中的规则，或者说是弱规则。

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

He made no offer of molestation orsociability, but kept aloof on one side of the road, jogging along on the blind side of oldGunpowder who had now got over his fright and waywardness.

他没有表现出任何好骚扰和爱社交的样子，而是孤伶伶的在路的另一侧，慢跑在绑火药的弱侧，他又开始变得恐怕和不安了。

To test the hypothesis, neontal rat cadiomyocytes Treat with heat shock(HS,42℃,2h) to induce the expression of HSP70,then teated with 0.5mmol/L H2O2. We first found H2O2 can reduced cadiomyocytes apoptosis and HSP70 only over-expression in the HS group, other two group only express HSP70 a little in the test of expression of HSP70.Then we tested the concentration of Ca2+, the [Ca2+ ] of CON was 192.224+6.654, the [Ca2+ ] of H2O2 group was 290.6918+8.922and the [Ca2+]of HS group was 214.2633+4.484.To further determine how HSP70 repaired the Ca2+ homestasis, we measured calcium transient of each group.

实验中应用MTT和流式细胞技术检测细胞的凋亡，发现损伤组的存活率明显低于其他2组，热休克组(Heat shock group，HS group)细胞存活率略低于正常组细胞；免疫组化结果表明只有在HS组才检测到阳性反应，即是HSP70的表达，其他2组均为弱阳性结果；应用离子成像技术对细胞内钙离子浓度进行测定，发现损伤组290.6918+8.922明显高于正常组192.224+6.654和HS组214.2633+4.484，HS组细胞内的钙离子浓度略高于正常组；为了进一步探讨HSP70对于ROS引起的心肌凋亡过程中钙离子的调控机制，又对各组的心肌细胞进行了钙瞬变的测定。

After comparing nonlinear simulation with linear, the results indicate that: Weak nonlinearity has definite and apparent influence on the distribution of acoustic fields and focused gain of array, which cause the distribution of waveform is closer to the array and the focused gain deteriorate ; the strong nonlinearity have a strong waveform aberration, because the other waves of non-fundamental frequency appear . No evident distinction is found in the effects between the oblique propagation and the vertical propagation, though less focused gain and influence caused by strong nonlinear produced by the acoustic diffraction which results in more attenuation in terms of oblique propagation.

将线性条件下的模拟结果与非线性条件下的模拟结果进行比较发现：弱非线性会对声场的分布和阵列聚焦增益产生一定的影响，使得声场分布波形比线性条件下的更加靠近阵列，聚焦效果变差；强非线性会使波形发生更严重畸变，这是由于产生了基频以外的其他频率声波引起的；非线性对斜向传播时声场分布的影响与垂直传播时的影响效果基本相同，但由于斜向辐射时的声波衍射造成的衰减要大于垂直辐射时的，因此聚焦增益和强非线性的影响都将小于垂直辐射时的情况。

My first article for Screen Power Magazine, which is to this day is the official magazine of Jackie Chan, was a DVD review of the Hong Kong actioner Gen-X Cops, starring Nicholas Tse and Daniel Wu.

我的第一篇文章为屏幕力量杂志，在对这天是Jackie Chan 正式杂志，是香港actioner Gen X 警察的DVD 回顾，担任主角Nicholas Tse 和丹尼尔吴。

His lips grazed hirs, Now what are you going to do?

他的唇使吃草了 hirs，现在你将要做什麼？