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The parameter of the reliability and the reproducibility of the microarray data were analyzed by correlation coefficient, coefficient of variation and false positive rate etc. Meanwhile, the error resource also had been inspected.

利用相关系数(correlationcoefficient, R)、变异系数(coefficientofvariation, CV)和假阳性率(falsepositiverate, FPR)分析猪瘟病毒cDNA芯片数据的可靠程度,对cDNA芯片实验数据作了整体的评估。

Self-comparison experiments with the same kind of tissue and differential expression experiments with the different kinds of tissue have been done to verify the reproducibility and the accuracy of this technique. The parameter of the reliability and the reproducibility of the micriarray data were analyzed by correlation coefficient, coefficient of variation and false positive rate etc. Meanwhile, the error resource also has been inspected.

通过同种组织RNA自身比较实验及不同组织RNA的差异分析实验对cDNA芯片实验的重复性进行检验,利用相关系数(correlation coefficient,R)、变异系数(coefficient of variation,CV)和假阳性率(false positive rate,FPR)分析cDNA芯片数据的可靠程度,对cDNA芯片实验数据作了整体的评估。

Self-comparison experiments with the same kind of tissue and differential expression experiments with the different kinds of tissue have been done to verify the reproducibility and the accuracy of this technique.The parameter of the reliability and the reproducibility of the microarray data were analyzed by correlation coefficient,coefficient of variation and false positive rate etc.Meanwhile,the error resource also has been inspected.

通过同种组织RNA自身比较实验及不同组织RNA的差异分析实验对cDNA芯片实验的重复性进行检验,利用相关系数(correlation coefficient,R)、变异系数(coefficient of variation,CV)和假阳性率(false positive rate,FPR)分析cDNA芯片数据的可靠程度,对cDNA芯片实验数据作了整体的评估。

Theoretical plates of propyl alcohol is 9.9×10^4 plates/m; resolution of ethanol and propyl alcohol is 4.66; RSD of correction factor of relative mass is 1.67% Good linearity with correlation coefficient 0.999 is observed over the range 45.0%~85.0% of ethanol in solvent. The method recovery for ethanol is 100.4% with RSD 1.69%; he RSD of accuracy is 1.74%.

结果:系统实用性实验测得正丙醇理论塔板数为99000,乙醇与正丙醇分离成4.66,相对质量校正因子的变异系数为1.67%;该方法在乙醇百分含量范围45.0%-85.0%内呈良好的线形关系,相关系数为0.999;平均回收率为100.4%, RSD为1.69%;精密度相对标准偏差为1.74%。

Theoretical plates of propyl alcohol is 9.9 ×104 plates/m; resolution of ethanol and propyl alcohol is 4.66; RSD of correction factor of relative mass is 1.67 % Good linearity with correlation coefficient 0.999 is observed over the range 45.0%-85.0%of ethanol in solvent.

结果:系统实用性实验测得正丙醇理论塔板数为99000,乙醇与正丙醇分离度为4.66,相对质量校正因子的变异系数为1.67%;该方法在乙醇百分含量范围45.0%-85.0%内呈良好的线形关系,相关系数为0.999;平均回收率为100.4%,RSD为1.69%;精密度相对标准偏差为1.74%。

The calibration curve of the SM2-Kit with standard SM2 inhibitor was typical sigmoid curve fitted to the four parameter logistic equation with the linear detection of 0.625 μg/L to 80.0 μg/L (R2=0.9911), the sensitivity of 0.9 μg/L, the IC50 of 5.82 μg/L and the detection limit of 1 μg/L. The recoveries of SM2 spiked in pig feed were 83.4 %, in pig urine were 89.5 %. The precision and accuracy of the assay as determined by inter-assay and intra-assay coefficient variation was both below 15 %. The SM2-Kit generally had 2.08 % cross-reactivity towards sulfamerazine and little or no cross-reactivity towards other sulfonamides. The dilution solution of SM2 had no effect on results of SM2-Kit. The validity of SM2-Kit in 4 ℃ was above six months.

研究结果表明,SM2-Kit的标准曲线呈典型的S型,相关系数R2=0.9911,符合4参数logit曲线拟合,线性检测范围为0.625 μg/L~80 μg/L,灵敏度为0.9 μg/L,半数抑制浓度(IC50)为5.82 μg/L,检测限为1 μg/L;饲料样、猪尿样的平均添加回收率分别为83.4 %、89.5 %,平均批内和批间变异系数均低于15 %;SM2-Kit与磺胺甲基嘧啶的交叉反应率为2.08 %,与其它磺胺类药几乎没有反应性;基质对SM2-Kit的检测结果影响不大;试剂盒在4 ℃可保存6个月。

Inner layer heterogeneity, plane heterogeneity and microfacies heterogeneity are studied by using heterogeneity parameters: permeability variance coefficient, permeability tantivy coefficient and permeability differential.

应用渗透率的变异系数、突进系数和级差来分析储层层内、平面非均质性与各微相的非均质性。

Results The coefficient of variation for the whole analytical method in the range of 31.83-2 037 mg/m^3 was

结果当空气中邻-氯甲苯的浓度范围在31.83~2037 mg/m^3(参照美国标准259 mg/m^3)时,方法的变异系数为1.2%~4.9%;回归方程式Y=3 399+135.0X,相关系数r=

ResultsThe within-day and day to day precision of Hitachi 7180 was both between 3% and 4%, the linearity was good (0.96~0.99), the schlepped polluting rate was 0, the coefficient of accuracy was higher than 95% and compared with 7600 autoanalyzer, the coefficient of correlation was higher than 0.9856, with no significant difference.

结果:日立7180日内及日间变异系数均在3%~4%之间;线性良好(0.96~0.99);携带污染率为0%;准确度在95%以上;与日立7600相比,相关系数在0.9856以上,无显著性差异。

Respective standard deviations, coefficients of variation, and average recovery were 0.18, 2.28%, and 99.09% for SYP-Z048, and 0.19, 0.59%, and 99.38% for thiram.

啶菌唑和福美双标准偏差分别为0.18和0.19,变异系数分别为2.28%和0.59%,平均回收率分别为99.09%和99.38%,线性相关系数分别为0.9896和0.9563。

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