变异比

During the postgenomics era the information about genomic sequence and gene functions provides a new foundation for evolutionary biology and ecology As the first whole-genome sequenced plant Arabidopsis thaliana and its wild relatives have played a critical role in understanding the evolution of genomics and speciation Both A halleri and A lyrata are closely related to the model species A thaliana A halleri ssp gemmifera occurs in northeastern China Japan and Taiwan; while its sister A halleri ssp halleri is mainly distributed in Europe Geographical barriers such as Tienshan Mountain Range isolate these intraspecific sisters Likewise A lyrata ssp kamchatica and ssp lyrata occur in East Asia and North America respectively Such distribution patterns seem to be consistent with allopartic speciation The comparison between ancestral and extant polymorphism by multilocus can be informative about the population genetics of speciation In this study we collected and analyzed DNA sequences of 98 genes from four wild relatives of A thaliana A halleri ssp gemmifera A halleri ssp halleri A lyrata ssp kamchatica and A lyrata ssp lyrata The ancestral states of these four species were compared to each other in terms of level of genetic variation However the ancestral species at the time of speciation were substantially more polymorphic than the extant geographical populations The observations are not fully compatible with speciation by strict allopatry At some species pairs parapatric speciation seems more reasonable in speciation of Arabidopsis The 98 gene sequences are also used for the congruence test between gene genealogy and species phylogeny Only 28 genes support the species phylogeny but there are 23 genes supports another major genealogy { lyrata} thaliana Based on the phylogenetic position change of A lyrata ssp kamchatica and Ks value for each species pair suggested the recent directional gene flow between A halleri ssp gemmifera and A lyrata ssp kamchatica

阿拉伯芥是第一个完成基因体定序的开花植物，其基因体资讯提供植物学研究的重要依据；在解析阿拉伯芥属物种的亲缘关系以及种化机制等重要的演化议题时，阿拉伯芥近缘的野生物种自然成了不可或缺的关键；跟阿拉伯芥近缘的物种包括A halleri及A lyrata，其中A halleri ssp gemmifera主要分布於中国东北、日本以及台湾，与近缘的A halleri ssp halleri其分布於欧洲隔著天山及大陆的障蔽，而A lyrata ssp kamchatica主要分布於东北亚及台湾，与分布於北美五大湖的A lyrata ssp lyrata被北极圈所分隔，这样的分布模式暗示异域种化的可能。藉由多基因分析比较祖先物种与现生物种遗传歧异度的相关可提供讯息探讨种化时期的族群遗传结构，本研究针对A halleri ssp gemmifera、A halleri ssp halleri及A lyrata ssp kamchatica、A lyrata ssp lyrata四个物种，两对互为亚种的姊妹群，以阿拉伯芥为外群进行研究，在四个物种完成98个同源基因的分子序列，利用套装软体MCMCcoal来估算祖先物种的遗传变异，亦估算现生物种的核苷酸歧异度，观察到？多物种配对中祖先物种遗传多型性大於现生物种DNA歧异度，显示异域种化模型并无法完全解释阿拉伯芥属物种的种化模式，在某些物种配对间邻域种化模式应比异域种化更为可能；在基因树与物种树的比较，98个基因片段的亲缘模式只有28个是与已知物种树一致的，有23个基因其树状图支持{ lyrata} thaliana的型式，藉由kamchatica位置的变化以及估算各物种配对间的平均同义置换率，推测在A halleri ssp gemmifera与A lyrata ssp kamchatica间具有近代的单方向基因交流。

Experiments of flow conductivity closure stress of typical propping agent are done, propping agent short-term flow conductivity alteration index is defined, by this, the relation between fissure short-term flow conductivity and closure stress, it is close to exponential relation. 4. Establishing the mathematical simulator of hydraulic fractured well's production in a steady state, finding the relation curve between dimensionless flow conductivity and fracture penetration coefficient by means of calculation and analyses, it is the basis of hydraulic fracture. 5. Expatiating the importance of optimized hydraulic fracturing design in reservoir engineering, advancing the optimizing design method, fracture dimension can be calculated easily when the physical parameters are known. 6. Production rate decline standard curve of vertical fractured wells is achieved by mathematical simulation, when the flow pressure is certain , by asymptotic analyze, this decline curve is close to exponential relation.

完成了国内外典型支撑剂的导流能力闭合应力影响实验，在数据分析中定义了支撑剂短期导流能力变异系数，并由此发现裂缝短期导流能力随闭合应力变化的近似符合指数关系式； 4 建立了一种水力压裂井稳态产能的数学模拟器，计算分析找到了最优无量纲导流能力与裂缝穿透比之间的新型关系曲线，它是水力压裂优化设计的基础； 5 阐述了水力压裂油藏工程优化设计的重要性，提出了优化设计方法，在已知支撑剂物性参数条件下可简洁计算最优裂缝尺寸； 6 数学模拟给出了垂直裂缝井产量递减标准曲线，在井底定压条件下通过渐近分析表明，该递减曲线满足指数关系式。

The several sets of arias with variations are, surprisingly, much, more developed than the organ chorale variations.

阿里亚斯与变异数集的，奇怪的是，许多较发达的比机关的乐章变化。

The thesis also draw several conclusions as follows:(1) Orthogonal experiment design method can be viewed as a special case of genetic algorithm, i. e. a genetic algorithm with a fixed initial population, an oriented-mutation operator and one evolution epoch.(2) In terms of running steps, genetic algorithm is more complex than orthogonal experiment design method.

论文首次对正交试验设计法和遗传算法这两种独立发展起来的方法进行比较研究，提出(1)正交试验设计法是遗传算法的一种特例，即是一种初始种群固定的、只使用定向变异算子的、只进化一代的遗传算法；(2)遗传算法产生的优化解优于正交试验设计法产生的优化解，且遗传算法处理交互作用项的效率高于正交试验设计法；(3)遗传算法的步骤比正交试验设计法复杂，所需的试验次数也要多。

The conserved regions of rRNA and tRNA genes exhibited higher sequence similarity among plant species. Five primer pairs were designed from the conserved regions distributing in psbA~trnK, psbB~psbH, rpl23~trnI, trnR-ACG~trnN-GUU and trnY-GUA~trnD-GUC, respectively to amplify sequences about 500~600 bp, 800~900 bp, 650~700 bp (350 bp, not legume species), 700~800 bp (400 bp in dicotyledon), and 500~600 bp.

经由上述比对分析后设计出5引子对，位於叶绿体基因体的位置分别为psbA~trnK、psbB~psbH、rpl23~trnI、trnR-ACG~trnN-GUU及trnY-GUA~ trnD-GUC，预期实际应用在其他物种上所能增幅之变异序列长度分别约为500~600 bp、800~900 bp、650~700 bp (豆科以外物种为350 bp)、700~800 bp (双子叶植物为400 bp)及500~600 bp。

The study has shown:(1) With increasing sediment depth and the woody giblets, the total organic carbon, the extraction of benzene , the total humic acids and the unhydrolyte are enhanced, while the hydrolyte is decreasing.(2) According to the GC-MS analysis, n-alkane,n-alkan-2-ones and n-alkanones are composed mainly of long-chains,such a result is thought to be characteristic of a herbaceous input from 2300a.B.P. n-alkanones exist in the samples may be considered as the evidence of microorganisms activity.(3) It is showed that the lipids is more sensitive to the climate change than the floristic. The proxy of the pollen spore of the peat stratum is different from the normal environment proxy. It is one of the main reasons that the sensitivity difference of the bog vegetation reacting to the climate isn't accordant with the log of the fat molecule fossil and biology fossil.(4) The transformation of the peat organic carbon is under the influence of temperature,acidity,organic material constituting, etc, and it showed the up-trend with the depth. In the short-term scale, the conversion of the peat organic carbon is effected by seasonal water,regional climate and vegetations; while in the long-term scale, it is controlled mainly by the climate.(5) According to the comparing the orderliness of organic compound,vegetation giblets with the depth to the characteristics of the peat molecule fossil with the section, in the development of this peatmire, 1500a B.P（210cm）is regard as the borderline of the late Holocene, the keynote of the front period is warm with several cold fluctuations, and the trend of the back period is dry and cold although there were several fluctuations.

研究表明：（1）随深度的增高及植物残体中木本残体的增多，总有机碳、苯萃取物、总腐植酸、不被水解物含量逐渐增高，而易水解物含量减少；（2）GC- MS分析显示，正构烷烃，直链烷基2-酮和正烷醇均以长链组分为主，揭示了该泥炭柱剖面2347a.B.P的母源输入以草本植物为主；甲基酮类和短链正烷醇的存在是微生物活性很大的生物证据；（3）通过有机分子标志物与孢粉、植物残体等指标的对比研究表明：脂类化合物对气候变化的反映要比植物种灵敏得多，不同沼泽植物对气候变化的敏感性的差异是脂类分子化石记录与生物大化石结果很难一致的主要原因之一；（4）泥炭有机碳的转化受到温度、酸度、有机物质组成等很多因素的影响，短时间尺度，泥炭有机碳的转化受季节性降水、地方气候和植被等因素的影响；长时间尺度，泥炭地有机碳的转化，受气候条件控制；（5）总结泥炭分子化石特征、泥炭有机质各组分含量及植物残体组成随深度的变异规律，分析结果显示该泥炭地层形成过程中，1500a B.P（210cm）可作为划分前后晚全新世的界线，前期的基调是气候偏暖，但又有几次偏干冷气候的反复，后期气候以干冷为主，其间虽有几次上升波动，但整体趋势不变。

The distribution of variance, exhibited severe skewness which can be adjusted by natural logarithm transformation. Table 1.Comparison TA with GE of HRV. Table 1. presented the RR interval、 Ln、 LF% and variance of two groups. TA had significant higher than GE.

在安静心跳率TA比NT及HT患者之心跳率低，且达显著水准；TA与NT及HT间R-R波间隔时间皆有显著差异；在Ln的结果，TA与HT有显著差异 NT与HT也有显著差异；LF%的结果，TA显著高於HT，NT与另两组皆无显著差异；心率变异度部份HT皆显著低於另外两组。

Compared to SOC density in the dry land, SOC density in paddy soils was 13 percentages higher with a lower variation coefficient between different regions.

不同用地方式下，水田耕层有机碳密度比旱地的平均高13个百分点，但水田有机碳密度的区域变异显著低于旱地。

In a whole, the genetic diversity of clonal plants is much higher than that had been expected before. Although the genetic diversity is lower than their sexual congener, widely genetic monomorphism has been rarely found.

总体而言，克隆植物拥有比早期推测大得多的遗传变异，虽然克隆种与其近缘有性繁殖种相比，遗传多样性较低，但广泛的遗传单态性却很罕见。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。