变型的

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Banana is a kind of typical respiration climacteric fruit, which has important theoretical and practical meanings to regulate its ripening and to study the mechanism of ripening.

香蕉是一种典型的呼吸跃变型果实，对其进行成熟的调控及机理的研究具有重要的理论和实践意义。

The normal feather Wugu chickens also had a lot of maternal ancestors; the result showed that at least six populations of red jungle fowl contributed to the gene pool of the domestic fowls.

丝羽乌骨鸡为常羽乌骨鸡的突变型，常羽乌骨鸡有比丝羽乌骨鸡更多的单倍型类型，而且在丝羽乌骨鸡群体中出现的几种主体单倍型类型也是常羽乌骨鸡群体的主体单倍型。

Six pairs of oligonucleotide primers were designed to amplify and sequence scarlet gene of mutant stbr flies which were obtained by outcross between Bar and wild type flies.

设计了6对引物对本实验室由棒眼果蝇Bar与野生型果蝇杂交所产生的亮红眼突变型果蝇stbr的scarlet基因进行了测序，结果在scarlet基因的第4外显子检测到了一个7.5kb的插入，进一步的测序证实此7.5kb插入为逆转座子412元件。

Objective To observe the influence of gene transfection of inducible nitric oxide synthase to the transplanted human laryngeal squamous carcinoma in nude mice, to investigate the expression and the correlayion of iNOS and p53, and to proke into the possible apoptosis mechanism. Methods Transfect the gene of iNOS to Hep-2 human laryngeal cancer cell, sieve out positive clone cell and amplify.

目的 观察诱生型一氧化氮合酶(inducible nitric oxide synthase， iNOS)基因转染对人喉鳞癌细胞裸鼠移植瘤生长的影响，研究在体人喉鳞癌细胞中iNOS与突变型p53基因的表达，探讨二者的相关性及促进肿瘤细胞凋亡的机制。

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折叠式铝合金展览架最大的优点是： 1、外观美观、细腻、本产品全部采用高强度铝合金材料，经过阳极处理； 2、承受力强、连接性强、安全系数高、不易变型； 3、运输携带方便、省运输费、搭建费及装潢费； 4、环保性强，不会产生大量的垃圾，一次性投资可重复使用，可自行维修、使用后可回收不会对自然产生污染，代替了世界上的木材资源； 5、多功能组合、组装简易、时间短、效率高、男女皆可、节省大量人工、费用； 6、造型多样化、可根据客户的需要定做S型、菱形、圆形、弧形等。

We thus hypothesized that increased TGF-beta signaling may contribute to the multisystem pathogenesis of MFS, including the development of myxomatous changes of the atrioventricular valves.

研究者使用抗游离和激活型TGF-β的单克隆抗体观察到，突变型小鼠二尖瓣叶内的免疫激活性增加，&表明TGF-β的激活而不是细胞因子的生成增多&。

Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group.

结果 免疫组织化学结果显示，Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关，对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值。

This answer, however, runs into trouble with a variant of the trolleyconnect with the track leading to the five people (1). Here we willsuppose that without a body on the alternate track, the trolley would

那么我们再来看一个&电车难题&的变型：假设站着一个人的那条轨道的另一端是跟另一条轨道相连的，即形成一个回路，如果那上面没有这个人，电车会从这条

The theory analysis, experiment test and simulation by computer are utilized to research the vibration characteristics of transporters converted from tractors in this paper.

本文运用理论分析、试验研究和计算机模拟相结合的方法，对拖拉机变型运输机的振动特性进行系统的研究。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。