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After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列,插入p16表达载体,并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因,构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

One year later, Slit was confirmed as the ligand of Robo and the Slit-Robo signaling is know involved in the repulsive effect of the commission axons crossing the mid-line, and also involved in the repulsive effect of the guidance of neuronal migration and leukocyte migration as well.

而对 Slit 功能展开的研究起于1998年,先是发现其受体 Robo突变型果蝇的中线处交叉神经元的导向发生缺陷。1999年的一系列遗传和生化的研究又发现,分泌性因子 Slit 正是 Robo 的配体, Slit - Robo 信号起到了排斥一些轴突,使之无法穿过中线的作用。

Marching cubes algorithm is a classical algorithm to extract iso-surface from regular volume data, and marching tetrahedra algorithm is improved based on MC algorithm.

Marching Cubes算法是基于规则体数据抽取等值面的经典算法,而Marching Tetrahedra算法是MC算法的改进和变型

MCP could effectively delay the softening of climacteric fruit, such as Starking and Fuji apples and Feicheng peach, but as different fruits had different softening mechanism, the mechanism of 1-MCP inhibiting different fruit softening was different: in Starking apples, the key enzyme to the softening is endo-and exo-PG, while during Feicheng peach fruit softening, the key enzyme was EGase before the 20th d and endo-PG after 20th d during storage at 0℃. 1-MCP inhibited fruit softening mainly by inhibiting PG activity in Starking apple while in Feicheng peach, by inhibiting the activity of EGase in the earlier storage time and endo-PG in the latter time. It was also found that LOX accelerated the initiation of the softening and was regulated by ethylene and 1-MCP in apple and peach.

2.1-MCP能较好的延缓红星、富士和肥城桃等跃变型果实硬度的下降,但是不同品种/树种的果实软化机理不同,1-MCP抑制其软化的机理也不一致:在红星果实中,对软化起主要作用的酶一直为内切和外切两种PG,而在肥城桃软化过程中,前20d以纤维素酶活性起主要作用,20d后软化以内切PG为主。1-MCP主要通过抑制红星果实中PG酶活性来抑制果实硬度的下降;在肥城桃果实中,1-MCP通过抑制前期纤维素酶和后期内切PG活性,延缓了果实硬度的下降。

S where 3-EtO-SalAPA is the monoanionic Schiff base derived from 3-ethoxysalicylaldehyde and N-(3-aminopropylaziridine and S is C6H5F, C6H5Cl, C6H5Br, C6H5I or o-C6H4Cl2. Their gradual nature of spin-state interconversion is evidenced by the variable-temp. magnetic susceptibility and EPR data. Only a single quadrupole-split doublet is seen in the Moessbauer spectra for all solvates in the temperature range of

本文对五种组成为[Fe(3-EtO-SalAPA)2]ClO4.S的Fe自旋变异晶态配合物进行了磁学和谱学表征,所有溶剂合物的变温磁化率和EPR数据证示它们的自旋态转变性质均属渐变型,在110-300K范围内的Mossbauer谱都只显示单一的四极分裂双峰。

With the increasing of the detritus content of rock masses(from mud shale, siltstone, to fine and medium sand), the compressive strength and elastic modulus of rock masses increase.

试验研究与理论分析结果表明,随着碎屑颗粒含量的增加,岩石的强度和刚性增强,受载过程中积蓄的弹性应变能增大而耗散的永久变形能减少,发生应变型冲击地压的可能性增加。

DDRT-PCR method was exploited to study the differential gene expression between immature siliques of Arabidopsis wide-type and ast mutant.

采用DDRT-PCR的策略,分析野生型与突变型植株未成熟角果中基因表达的差异。

The plane-parallel resonant cavity is built up in theory with the U-shaped bending waveguide for TM mode. The beam splitting and convergence effect are also discussed for the structure composed of filling-factor graded wave-like 2D-PC and one-dimensional multilayer thin films.

利用TM模的U型转弯波导构建了新型的平行平面谐振腔,并指出由填充率渐变型波状结构二维光子晶体和一维多层薄膜构成的混合结构在光分束和抑制光束发散方面的作用及其在平行平面谐振腔中的潜在应用。

Results:Twenty four cases of β thalassemia with CD17 mutant including 3 cases in Shanxi, 20 cases in Gansu and 1 in Xinjiang, were found among 85 probands of β thalassemia.

结果:对该地区的85例β地贫先证者进行了基因分析,检出24例CD17突变型,其中陕西3例、甘肃20例、新疆仅1例,以甘肃地区检出率为最高。

Based on product platform, many design candidates were produced by making use of inheritance, mutation, as well as crossover of evolution technology.

在产品平台的基础上,利用进化技术中的遗传、变异和交叉等方法进行产品设计,产生更多的变型产品设计方案。

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推荐网络例句

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。