变型

The tryptophan auxotrophs and the kanamycin resistant prototrophic revertants have been proved to contain a copy of Tn5 inserted in the chromosome by Southern Blot analysis with DIG-labeled 3. 5 kb HindⅢ fragment of pkan2 as the Tn5 probe. Four typies of prototrophic revertants could be found by the above assay.

用Tn5探针进行的Southern分子杂交证明Tn5在色氨酸营养缺陷型菌株及部分Km抗性原养回复突变型菌株中均插入在染色体DNA上，并且对菌株本身具有的内源性质粒的数目及大小没有带来影响。

Twenty-eight prototrophic revertants of the tryptophan auxotrophs were obtained through spotaneous back mutation from colonies grown on synthetic medium plates or nodules of plants. Most of the revertants have resumed the nodulation abilities and enhanced nitrogen fixation activities as evidenced by darker green color, higher dry weight and more nitrogen contents in shoot than those of the wild type strain 8-1-4A did in pot experiments. However, noodulation abilities decreased to some extent and no significant differences were found in the mass of nodules compared with the parent strain.

通过自发回复突变，从培养平板和根瘤中分离到28个色氨酸原养回复突变型菌株，对其中25株进行植物盆栽试验证明其中24株菌的结瘤功能得到了恢复，而且固氮酶活性普遍比出发菌有所提高，其中41％达到极显著水平，18％达到显著水平，植株地上部分干重及含氮量也有不同程度的提高，唯有根瘤数比出发菌有所降低，其中23％降低达极显著水平，11％降低达显著水平。

According to therelationship of person and animal, the images can be divided into three kinds:prototypic animals, transformable animals and opposite animals.

根据人与动物的关系，将动物形象分为三类：原型动物、转变型动物、对立型动物。

After that, a Variant-Design-Oriented Quantitive Product Definition Method is brought out with its process and tools discussed in detail.

提出了面向变型设计的量化产品定义方法，阐述了该方法的实现过程和数学工具。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Module construction which is the major task in modular design of military equipments, is aimed at designing standard, general-utility and seriate modules which fulfil the requirements of basic design, variant design and unitized design.

模块创建是装备模块化设计中的核心环节，其主要任务是要设计出标准化、通用化和系列化的模块，满足装备基型设计、变型设计以及组合设计的要求。

The results show that NPT II had already integrated into the genome of Sinningia speciosa, and the transformation efficiencies of tMEK2 and tMEK2MUT were 1.7% and 0.7%, respectively.

野生型、突变型MAPKK基因的转化效率分别为1.7％和0.7％。

Three expression vectors containing mutant gfp which were truncated from 3′ termine were constructed in the present paper.The mutation gfp with different length under the control by T 7 promoter were expressed in E. coli BL21 cells.

本文将突变型 gfp3′末端不同程度地截短，构建成表达载体，在大肠杆菌 BL 2 1中表达了不同长度的绿色荧光蛋白，当其生色基因中 65位 Ser点突变成 Thr，则 GFP的激发波由 396nm增至 4 70 nm，不用 UV激发，仅在自然光源下就可看到强烈的绿色荧光，发光强度增加 6倍，若从 3′端截短 12个氨基酸密码，对发光无影响，但截短 75个氨基酸密码，则激发波长降到 360 nm，发光强度减少 12倍。

This procedure is suitable for liquids with non-Newtonian behavior, i.e., liquids that are either shear-thinning or thixotropic.

此过程是合适于非牛顿体的液体。液体是可以剪切稀或触变型的。

This paper constructed a transformable structure of the products, accounting for the functions and characteristics of the system; brought forward a referenced optimization scheme for implementation.

通过构造一种可变型的产品结构，说明该管理系统应具备的功能和特点；对系统具体实施过程，提出优化实施方案。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。