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变圆

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Results hypso-purity (95%) astrocytes can be acquired and more depurative by passaging, part of astrocytes will be dying after nulli -nutrition;If applying methylprednisolone (10umol/l), cytoactive of damaged astrocytes will obviously super and expression of GFAP also obviously increase, all the thing is opposite for normal astrocytes.

结果 星形胶质细胞摇床后传至第三代,经鉴定其星形胶质细胞纯度达95%以上;缺氧3h后部分细胞形态变圆甚至死亡漂浮在正常细胞表面;正常组和损伤组分别加入MPSS3d后,损伤组细胞活性与GFAP表达均明显升高,但是正常则没有明显变化。

Results Stable drug-resistant SW1990/GZ cell strain was established by culturing with gemcitabine for 9 months. The morphology and growth characteristics of the cell strain changed remarkably. The cells shrinked and became rounder; its endoplasm expanded; granular substances increased; and the doubling-time was prolonged. Resistance of the cell line to gemcitabine, fluorouracil, adriamycin, and mitomycin significantly increased. The TrxR activity of the drug-resistant cells was increased markedly.

结果 药物培养9个月后,得到稳定传代的SW1990/GZ耐药细胞株;其形态学、生长特征等均发生了明显变化,细胞变圆、体积缩小、内质网扩张、颗粒样物质增多及细胞倍增时间延长;对吉西他滨、5-氟尿嘧啶、阿霉素和丝裂霉素的耐药性均显著增加;耐药细胞的TrxR活性也明显增高。

The embryonic vessels failed to invade into the labyrinthine layer of placenta, which impaired the embryonic-maternal vascular connection. These defects could not be rescued by wild-type tetraploid blastocysts, excluding the possibility that they were due to the extraembryonic tissues.

组织免疫组化分析结果显示:毛细血管腔异常扩张,排列紊乱,不能形成大血管及细致有序的血管网络;胚外中胚层来源的卵黄囊脏层扁平上皮细胞形态变圆,与内皮细胞连接受损;胚胎血管不能侵入胎盘迷路层,胚体与母体血管连接受损。

Expression of hepatocyte markers such as ALB and CK18 was measured with immunohistochemistry. RESULTS: Cells in control group kept the long spindle shape, with shape agent as 4.5±1.0 and small size, proliferated quickly, submitted typical fibroblast whirlpool or flamboyancy growth. Five to seven branches were observed at the late culture period, no cell rounded, and the rate of dikaryocyte was less than 0.1%.

结果:未进行诱导的对照组细胞保持长梭型,形状因子4.5±1.0,面积较小,增生较快,呈典型成纤维细胞的漩涡状或火焰状生长,培养后期可见5~7个分支,无细胞变圆,双核细胞率小于0.1%,逐渐老化,颗粒增多。

My head become more round which occupies half of my body.

我的头逐渐直起来,而且开始变圆

The CNE2/Gem cells appeared roundish in various size under light microscopy, with more granulation in cytoplasm.

光镜下见CNE2/Gem胞体变圆,大小不一,胞质内有较多颗粒。

Dexamethasone treatment group, the hearts showed cardiac musclewaxy necrosis, bubble apomorphosis, the livers showed a large area of cellularnecrosis, the spleens showed hemorrhagic infarct, the lungs showed hemorrhage andhyperemia, serous fluid exudation, the kidneys showed granular degeneration, thecerebrum showed nerve cell swelling.

地塞米松治疗组的心脏表现为心肌发生蜡样坏死,水泡变性;肝脏表现为肝组织出现大面积坏死;脾脏表现出血性梗死;肺脏表现为出血充血,浆液渗出;肾脏表现为颗粒变性;大脑表现为神经细胞肿胀,变圆

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下:一、tau蛋白过度表达和聚积对细胞形态的影响:倒置显微镜下观察两种细胞的形态,发现N2a/wt细胞的突起多而长,而N2a/tau40细胞胞体变圆,突起明显缩短;免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍,免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光,tau主要分布在核周和突起起始部分的胞质内,而N2a/wt细胞内的荧光很弱。

The correlations among the parameters suggested that larger trophozoite had its shape closer to round but the shape of nucleus changed little.

参数间的相关系数显示,随着滋养体增大,虫体的形态变圆,而核的形态变化不大。

Look, from chinese new year until now, my waist is more unshaped and my face is more and more round like asa water melon.

哈哈~~看看现在的自己,由过年至今,腰粗了,面也变圆了像个大西瓜一般。

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