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The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中,采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术,系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化,T和B淋巴细胞、T淋巴细胞亚群的动态变化规律,淋巴细胞表面某些粘附分子的变化,淋巴细胞和神经元的坏死与凋亡,雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律,脑组织中围官性细胞浸润的细胞类型。

Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.

用三种不同毒力的钩体(致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株)分别感染体外培养的豚鼠腹腔巨噬细胞,并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min,通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数,检测钩体对巨噬细胞吞噬功能的影响;感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化;用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。

Wear degree decreases, and the wear particle distribution curves become protrusive and precipitous gradually in the initial stage.

通过分析铜合金销与碳钢盘在干摩擦条件下相互对磨所产生的磨粒群和销试样磨损量,发现磨粒的累积分布和微分分布特性随磨损时间的变化而变化:在磨损开始阶段,磨损程度逐渐减小,磨粒群分布曲线由平缓变得逐渐凸、陡;达到磨损平衡状态后,磨损率达到最小,磨粒群微分布曲线的幅值达到最大,横向宽度达到最小;随着销与盘间互适性变弱,磨损程度增大,磨粒分布曲线变得越来越平缓,横向宽度逐渐增大;磨粒分布曲线随磨损时间的变化规律与磨损率随磨损时间的变化规律有明显的对应关系,可为科学诊断和预测摩擦学系统状态提供有用信息。

Based on the Arcview platform and compiling programs by ourselves, the DLPM of urban area of Jinan in 1998 and 2001 is established. The main conclusions are: 1 general trend of land price is reduction from central base point to urban edge, but there are different characteristics in different direction; 2 from one edge to another, land price shows low-high-low, and its sections through urban areas show normal school and deflection normal school; 3 land price evolutes with time, concaveness land price terrace changes little or gentle or protruding, and protuberant land price terrace changes little or not, price terrace or big or gentle; 4 the increasing potential of price is analyzed by the increasing rate and increasing speed of land price.

分析数字地价模型得出结论:①地价从中心基点向城市边缘总趋势是降低,但不同方向表现出不同的特点;②在贯穿市区的地价剖面中,一般表现为从一个边缘到另一个边缘的地价由低到高再到低的变化规律,由於地价剖面经过不同的市区区位,呈现出正态和偏正态的分布特点;③在时间演化上,凹性地价阶地变小或变平缓,凹性地价阶地转变为凸性地价阶地,凸性地价阶地变小或转化为非地价阶地,凸性地价阶地变大或平缓;④利用地价增值率和地价增值速度分析了地价的增值潜力。

The result makes clear, 2 class give urban sewage plant on the molecular quantity interval that organic owner should center the dissolvability in water water in kDa, and the content of organic matter compares hydrophoby the element of content big; of organic matter measures hydrophily interval distributings different organic thing shows water to accept filter film flux and hold back rate having distinct effect, the organic matter under molecular quantity 2kDa causes accept filter film to appear water flux attenuation bigger, organic matter is held back rate is minor, the organic matter that amounts to 57.2%; element to measure 10 ~ 100kDa causes accept filter film to appear water flux attenuation gentlier, organic matter is held back rate is older however highest the organic matter that amounts to 73.8%; to kiss scanty sex to differ shows water to accept filter film flux and hold back rate having distinct effect, classics is right of velar surface obstruction determine, confirm cause film to appear water flux and the main material that hold back rate change to be hydrophoby material, and the change that hydrophily material causes is minor.

结果表明,城市污水厂二级出水水中的溶解性有机物主要集中在kDa的分子量区间上,且疏水性有机物的含量比亲水性有机物的含量大;分子量区间分布不同的有机物对纳滤膜透水通量和截留率有着不同的影响,分子量2kDa以下的有机物引起纳滤膜透水通量衰减较大,有机物截留率较小,达57.2%;分子量10~100kDa的有机物引起纳滤膜透水通量衰减较平缓,有机物截留率却较大最高达73.8%;亲疏性不同的有机物对纳滤膜透水通量和截留率有着不同的影响,经对膜表面阻力的测定,证实引起膜透水通量和截留率变化的主要物质为疏水性物质,而亲水性物质引起的变化较小。

Therefore, it has the important scientific research value in studying the relationship between the changing of the parameters (including the temperature, the humidity, the grain radius and so on) and the electricity and radioactivity of the medium. Applied the count statistics and the numeral value analysis, the paper analyzes, concludes and contrasts the data sufficiently through the experimentation, and acquires the following results that the resistivity and the polarizability both have the negative relationship with the temperature variety of medium from 0℃ to 200 ℃. The parallactic poalarizability is consistent with the temperature variety in the plan as well as the section, but their trends are exactly opposite. Power supply voltage has no considerable influence to the resistivity and polarizability by using duality variance analysis, however, the detecting depth and the plan position influence them greatly. Different AB/2 interval that increases by degree does not influence variance and the average value of the resistivity and the polarizability remarkably. By using the least double multiplication, the relationship of temperature-time-the influence radius of the fire source is carried up by the mathematic drawing up. After discussing their correl ation, I educe the influence radius of the fire source and carry out the 'trend analysis and forecast to the spacial changing of the temperature and the parallactic polarizability in the end.

本文通过试验并应用数理统计和数值分析等相关理论对所得到的试验数据进行了分析、归纳和对比,得出了介质温度变化在0-200℃内视电阻率和视极化率与温度均表现为负相关性;视极化率与温度在剖面和平面上的变化形态具有很好的一致性,但变化趋势相反;由二元方差分析可得供电电压对视电阻率和视极化率无显著影响,但探测深度和平面位置对视电阻率和视极化率有显著影响;通过假设检验可证不同AB/2递增间隔,对视电阻率和视极化率的方差和平均数影响都无显著性差异;应用最小二乘法对温度——时间——火源影响半径之间的关系进行了数学拟合,讨论了它们之间的相互关系,得出了火源影响的有效半径,太原理工大学硕士研究生学位论文并对温度和视极化率的空间变化作了趋势分析和预测。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示:(1)SOD活性在52.98~133.20 U·g-1·h-1之间,并在早期单胚形成后一直保持上升的趋势,表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关;(2)POD活性在0.05~0.50 U·mg-1·min-1之间,呈现出先下降后升高的趋势,在胚性愈伤组织中最高,而在后期单胚形成时降至最低,表明其与原胚团的分裂和分化呈正相关,但与PEMⅢ向后期单胚的发育呈负相关;(3)CAT活性在0.86~2.81 U·mg-1·min-1之间,表现出升-降-升的变化趋势,在早期单胚形成时升至最高,在早期子叶胚形成时降至最低,表明其与早期单胚的发育呈正相关,而与中期单胚和早期子叶胚的发育呈负相关。

The FosB/△FosB positive staining was detected in nucleus accumbens septum, tuberculum olfactorium and dorsal caudate putamen, without little cFos positive staining. The results indicate the expression of cFos and FosB/△FosB display the differences in area and intensity, and the area intensively expressing FosB/△FosB is more nearly coincide with the area related to schizophrenia. So FosB/△FosB is a better mark to research the pathogenesis of schizophrenia.

同时大量研究证明,动物模型的行为及生理变化与中枢神经系统神经元的病理变化有关,因此,利用即早基因反应的灵敏性、功能多样性、检测方便性等,应用免疫组织化学的方法对模型小鼠脑内cFos和FosB/△FosB蛋白的表达进行检测,通过确定不同脑区cFos和FosB/△FosB蛋白的表达,来研究哪些脑区或神经元参与了反应,从而发现精神分裂症在脑区内所涉及的解剖结构,有助于对精神分裂症发病机制的认识。

The CVD is an important index to indicate tree diameterdifferentiating.On basis of dynamic change and tendency of CVDamong trees in stand,three models of square CVD are proposed.i.e.,basic model,stand factor model,and thinning effects model.All threemodels are of mechanism,asymptotic characteristic,and invariance forprojection length.Futhermore,there are compatiability and extremevalues in thinning effects model for thinned and unthinned stands.

直径变动系数是描述林木直径分化的重要指标,根据林木直径变动系数的动态变化和趋势,本文提出了直径变动系数平方动态变化的三个模型:基本模型,与林分因子相关的模型,考虑间伐效应的模型,上述三个模型均具有一定的机理性、渐近性和步长不变性,特别是考虑间伐效应的模型为间伐林分和未间伐林分的相容性模型,它还具有相容性和极值性。

The compound solution performs as pseudo-fictile fluid, and its visual viscosity is reduced when the shearing velocity increases. The higher the cationic degree of CPAM, the more apparent the rarefying behavior of the polymer-surfactant complex, but it still is pseudo-fictile fluid. In addition, such factors as the molecular weight of CPAM, temperature and PH value etc, have different effect on the properties of the mixed solution, including its visual viscosity, conductivity, rheological behavior and so on.

CPAM/SDS复合体系的表观粘度随剪切速率的增大而下降,即具有剪切变稀行为的特性,表现为拟塑性流体:CPAM的阳离子度越高,它与SDS形成复合体系剪切变稀的行为越明显,但体系的流动类型不发生变化,仍为拟塑性;随着CPAM分子量的提高,复合体系的非牛顿性增强;温度的升高将导致复合体系的粘度显著降低,非牛顿性减弱;对于SDS用量低于等电荷当量的复合体系,pH值的变化对其流变性的影响不显著。

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推荐网络例句

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性,但我大喊,并在他们的方向扔石头让他们过的线索,远离我,以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。

Store this product in a sealed, lightproof, dry and cool place.

密封,遮光,置阴凉干燥处。