受精卵核

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子体产生的两个精子中的一个进入颈卵器与卵细胞结合形成受精卵，受精卵核到第3次分裂形成细胞壁，没有受精的卵细胞还要进行两次有丝分裂后才消失。

At fertilization one male nucleus fuses with the gee nucleus to form the zygote, while the second male nucleus fuses with the primary endosperm nucleus to form a triploid cell, or more rarely a second diploid or a pentaploid cell that later gives rise to the ENDOSPERM.

受精作用发生时，一个精核与卵核融合形成受精卵，第二个精核与最初胚乳核融合，形成三倍体细胞，偶而也形成第二个二倍体或五倍体细胞，三倍体细胞最后发育为胚乳。

Double fertilization The fusion of one pollen nucleus with the egg to form the zygote and of the other pollen nucleus with a polar nucleus to form the triploid endosperm nucleus.

双受精：指花粉粒中的一个精子与胚珠中的卵细胞结合形成受精卵，另一个精核与极核结合形成三倍体的胚乳核。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

The hybrid between Brachymystax lenok and Hueho taimen were not a live in the past 2 years. However, the fertility and eyed rate of BH were significantly higher than that of HH and BB (P.05), while the hatching rate were significantly lower (P.05). The larvae died within 1 to 2 hours after hatching. It was observed that the tail of yolk-sac larve couldn't stretch around the yolk, and there were hyperemia in the middle and posterior segment of the tail. The reason why the hybrid between Brachymystax lenok and Hucho taimen couldn't snrvive might was the karyoplasms of the gametes from Brachymystax lerwk and Hucho taimen by distant hybridization were not compatible.

细鳞鲤×哲罗鱿的杂交经过2年多批次实验均未得到成活杂交子代，但在胚胎发育阶段BH的杂交受精率、发眼率均显著高于双亲自繁对照组(P.05)，但其孵化率显著低于双亲对照组(P.05)，BH杂交子代在破膜后1~2h内即死亡，刚破膜的仔鱼尾干中后段至尾鳍部分盘绕于卵黄囊表面不能伸展，且所有破膜仔鱼的尾干中后段均存在充血点，本研究认为，这种反交［细鳞鲑×哲罗鲑］子代不能成活的原因可能是由远缘杂交受精卵核质不相容所导致。

Over expressive mutant lamin A is toxic to the development of zygotes, Only zygotes with no target gene integration could survive until to be born.

过量表达的突变核纤层蛋白A对小鼠受精卵有致死性，只有无目的基因整合的受精卵才能存活至出生。

The results of protein synthesis of NT embryos indicated that the protein synthesis pattern of NT embryos and fertilized eggs was similar. 19. 2 KD protein existed in 32-cell stage donor embryos disappeared in NT embryos at the pronuclear stage, however, this protein was reprogrammed to reappeared in 32-cell stage NT embryos.

七、核移植胚的蛋白合成分析结果表明，原核期核移植胚与原核期受精卵具有相同的合成模式。32-细胞期供体胚中的19.5KD蛋白在原核期重组胚中消失，而在32-细胞期重组胚中再度出现，发生了蛋白合成的重序过程。

In vitro associstion experiments indicate that the plasmid pAy6.8 containing the core region of Antheraea yamamai fibroin gene can tightly bind to the nuclear matrix of Bombyx mori fertilized eggs,and recognize the same binding sites of homogeoous MAR fragments.

体外结合实验表明，携带天蚕丝素基因核心区的质粒pAy6.8可与家蚕受精卵核基质紧密结合，而且同内源MAR片段竞争结合位点。

The fertilization was processed by sperm in mature male gametophyte combining with egg cell on the top of archegonia on June 15 or so.

摘要樟子松发育成熟的雄配子体中的精子6月15日左右在颈卵器中上部与卵细胞结合，进行受精作用，其后，受精卵进行游离核分裂，形成8个子核时，开始形成细胞壁。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。