受精卵

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of 8 female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

6 Kb of pGFAP-Cre-hGH DNA fragment was introduced into 191 fertilized eggs by microinjection. 176 injected eggs were implanted into the oviducts of eight female mice respectively, from which 25 offspring were obtained.

以显微注射的方法将7.6 kb的转基因片段pGFAP-Cre-hGH引入191枚小鼠基因组受精卵，其中176枚分别移植至8只假孕母鼠的输卵管中使其发育，共获得子代小鼠25只。

Methods Totally 630 zygotes were collected from the oviducts of superovulated female Kunming mice, which were cultured in protein-free potassium simplex optimized medium supplemented with Eagle's essential amino acids and Eagle's non-essential amino acids of different concentrations (mKSOM, mKSOM+1/16AA, mKSOM+1/8AA, mKSOM+1/4AA, mKSOM+1/2AA, mKSOM+AA, and mKSOM+2AA).

方法从超排昆明小鼠输卵管中取出的630枚受精卵均分为7组，分别在经添加不同浓度Eagle's氨基酸的去牛血清白蛋白KSOM培养液(mKSOM、mKSOM+1/16AA、mKSOM+1/8AA、mKSOM+1/4AA、mKSOM+1/2AA、mKSOM+ AA、mKSOM+2AA)中培养，对比观察各组胚胎体外发育的过程。

However, crossing fertility between them was lower than that of mating in and in, and fertilized egg of reciprocal crossing can be incubated and produced F〓 without prezygotic isolation. Among all combinations of reciprocal crossing, crossing of wild male quail×female small-type domestic quail are proved to produce the best effect.

野生日本鸣鹑与家鹑群体之间的杂交能育性低于同种群交配水平，但可以实现正、反交，产生受精卵、孵化出杂种一代，不存在合子前生殖隔离；在所有正、反交组合中，以野雄鹑×小型雌家鹑的效果最好。

Results of interpopulation crossing test of mating, fertilization rate, embryo growth and hatchability under the condition of cage feeding indicated that:(1) due to difference in body size, morphology, habits and characteristics and reproducing season, crossing fertility between wild Japanese quail in China and domestic quail population was lower than that of intrapopulation mating, but fertilized egg through reciprocal crossing could be incubated and produced F1 without prezygotic isolation;(2) crossing of wild male quail ?female small-type domestic quail produced the best effect among all combinations of reciprocal crossings.

通过关于笼饲环境下交配行为、受精率、胚胎发育、种蛋孵化率的种群间杂交试验证明：由于体量、形态、习性、繁殖季节性的差异，我国境内的野生日本鸣鹑与家鹑群体之间的杂交能育性低于同种群交配水平，但可以实现正、反杂交，产生受精卵、孵出杂种一代，不存在合子前生殖隔离；在正反杂交组合中，以野雄鹑X小型雌家鹑的效果最好。

To elucidate the mechanisms regulating TSHb expression, we isolated a 5.1-kb fragment of the red-spotted grouper 5'-flanking region by Genome walking .1.8kb of this upstream region was used to produce GFP reporter construct (pBK-TOL2) for analysis of tissue-specific expression in zebrafish embryos.

将起始密码子上游1864bp的启动子片段顺向克隆到pBK-TOL2载体中， pTSHb-TOL2重组质粒与转座酶mRNA共注射斑马鱼一细胞期受精卵，荧光显微镜观察发现，TSHb启动子驱动GFP在原始生殖细胞和垂体中表达。

He said his team took skin cells from a beagle, inserted fluorescent genes into them and put them into eggs before implanted them into the womb of a surrogate mother, a local mixed breed.

他说，他的研究组从一只猎犬身上提取皮肤细胞，并植入荧光基因，再将其注入卵细胞形成受精卵植入代孕母狗的子宫内。这只母狗是当地的一个杂交品种。

He said his team took skin cells from a beagle, inserted fluorescent genes into them and put them into eggs before implanted them into the womb of a surrogate mother, a local mixed breed.

他说，他的研究小组从一只猎犬身上提取皮肤细胞，并向细胞中植入荧光基因，再将其注入卵细胞形成受精卵植入代孕母狗的子宫内。

He said his team took skin cells from a beagle, inserted fluorescent genes into them and put them into eggs before implanted them into the womb of a surrogate mother, a local mixed breed.

他说他的小组从猎狗身上提取了皮肤细胞，植入荧光基因并把细胞植入受精卵，再放入本地杂交代孕母亲体内

By contrast, most miscarriages occur earlier in pregnancy, usually within three weeks of conception.

研究人员得出结论说，在妊娠开始的最初阶段，也就是当受精卵处于着床的过程中时，孕妇的精神压力的大小对怀孕能否顺利进行影响巨大。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！