受精卵

In addition, we studied the distribution of mitochondria in metaphase Ⅱ rabbit oocytes, zygotes and early embryos by two active mitochondria dyes and revealed the change of active mitochondria during early development of rabbit embryos. We also found that the distribution of microfilaments and microtubules have similar patterns to that of active mitochondria, which is an important basis for further development of animal cloning study using the recipient oocytes derived from rabbit.

此外，我们应用两种线粒体荧光染料研究了日本大耳白兔体内成熟卵母细胞、正常交配后收集的受精卵及早期体外发育胚胎的活性线粒体的位置变化，首次揭示了兔早期胚胎中活性线粒体的变化特征及其与微丝微管变化的相关性，为进一步开展以兔卵母细胞作为核移植受体的动物克隆研究打下了重要的基础。

As the wrigglers develop, the gelatinous outer membrane decomposes, perhaps triggered by a chemical change in the tadpoles. One by one, they slide off to a life of their own in the water.

受精卵一天天长大，卵胶膜破裂--可能是蝌蚪体内发生了某种化学变化--于是，一只接着一只，它们滑入下方的水中，开始自己的生命周期。

She's gone from egg to embryo, to fetus, to trillions of cells of newborn baby.

她从一个受精卵开始，经历了从胚胎，到胎儿，到几千亿个细胞组成的婴儿的过程。

Methods:The HBVtransgenic mice were prepared by microinjection of complate 2.0 copy HBV genome into pronuclei fertilized eggs.

应用受精卵原核显微注射的方法，产生HBV转基因小鼠。

Offspring of the fertilized eggs whose pronuclei were injected of the plasmid were screened for transgene expression.

接著此载体以显微注射递送到小鼠受精卵中，其子代以聚合脢链锁反应筛选转殖基因是否嵌入其基因体。

Then, the two recombinant genes were microinjected into the male pronuclei of fertillized mouse eggs for generating transgenic mice harbouring the introduced genes, respectively.

进而将它们分别导入小鼠受精卵雄原核，作成相应的转基因小鼠，则发现它们都失去了表达特性。

PKC isoform was typically concentrated around the condensed chromosomes before the MI stage, but localized in the pronuclei of zygotes and the nuclei of blastomeres of early embryos.

PKC亚形集中在MI期前浓缩的染色体周围，也位于受精卵原核或早期胚胎分裂球的核。

Early cleavage rate,embryo quality,the rate of blastocyst,implantation and pregnant rates from these zygotes were observed,and the transferred embryos were selected according to the pronuclei orientation and early cleavage time.

根据受精卵原核构型的不同分为四组，结合构型类型及早期卵裂与否进行选择性移植，通过观察优质胚胎率、囊胚形成率、种植率和怀孕率，来评估合子原核构型和早期卵裂与胚胎发育潜力的关系。

We spliced the pronuclei vector and microinject the remain DNA sequence to pronuclei of the mouse zygote.We got 29 mice of G0 generation ,and 8 of the 29 mice are positive result which checked by method of PCR.

构建的乳腺特异性表达载体去除原核部分(αS1-casein基因调控序列+人胰岛素原基因)，显微注射小鼠受精卵雄原核，获得29只G_0代小鼠，PCR检测出其中8只为转基因刚性小鼠。

This study was conducted to clone the sheepβ-lactoglobulin gene and the human myelomonocytic surface differentiation antigen CD14 gene (hCD14) by PCR, and this two gene were fused to construct oBLG-hCD14 fuse gene, this fuse gene were microinjected into pronuclei of mouse eggs to produce transgenic mouse.

本研究拟利用PCR和分子克隆技术，首先获得绵羊β-乳球蛋白基因调控序列片段和人CD14基因（hCD14）全程编码序列，并构建oBLG-hCD14融合基因，最后利用微量注射法将融合基因导入小鼠受精卵以产生转基因小鼠。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。