受精卵

In order to obtain viable tetraploids, which remains a major challenge worldwide, research on spindle organization and chromosome behavior was carried out mainly in zhikong scallop, Chlamys farreri, a typical commercially important species which is being cultured in North China Sea. Immunofluorescent technique and laser scanning confocal microscopy has been developed for observation of both microtubule organization and chromosome segregation. Orcein squash method was also used for chromosome observation. A series of complicated configurations of the meiotic spindle and associated patterns of chromosome segregation, including tripolar and seperated bipolar segregation, were found in developing eggs following the inhibition of the first polar body.

为了攻克可存活四倍体人工培育技术的国际性研究难题，本研究选取了我国北方海区的典型经济贝类栉孔扇贝为主要研究对象，建立了一套免疫荧光染色和激光扫描共聚焦显微技术，结合使用染色体地衣红压片观察技术，对第一极体受抑制的受精卵的减数分裂纺锤体装配、染色体分离动态进行了研究，发现了一系列特殊的纺锤体装配及相应的染色体分离行为，包括三极分离、独立二极分离等分离类型，从亚细胞水平上阐明了四倍体形成的细胞学机理。

The ova may be fertilized at oviducal gland and then the thick brown rectangle egg c...

卵经过卵壳腺时受精，受精卵外被一厚而坚韧的卵鞘，为黄褐色、椭圆四角形

Then one ovule degenerated, the other which contained zygote kept on developing. Embryo undergone proembryo and globular embyo stage in early part of June. Heart stage embryo developed into torpedo stage, and meanwhile nuclear endosperm formed cellwalls in later June. Birch Fruit matured in July..

此后，一个胚珠退化，另一个含有受精卵的胚珠继续发育。6月上旬，白桦胚正经历原胚球形胚阶段。6月下旬核型胚乳细胞化，心形胚发育为鱼雷胚。7月，白桦果实成熟。

The fertilized egg at the pronuclear stage (prior to the first cell division) is then placed into the fallopian tube hoping that the tubal environment, just as in GIFT, will aid in embryo development, growth and pregnancy.

将原核期的受精卵移植回输卵管环境，此时就同GIFT一样了，这将有助于胚胎的发育。生长和妊娠。

The results of protein synthesis of NT embryos indicated that the protein synthesis pattern of NT embryos and fertilized eggs was similar. 19. 2 KD protein existed in 32-cell stage donor embryos disappeared in NT embryos at the pronuclear stage, however, this protein was reprogrammed to reappeared in 32-cell stage NT embryos.

七、核移植胚的蛋白合成分析结果表明，原核期核移植胚与原核期受精卵具有相同的合成模式。32-细胞期供体胚中的19.5KD蛋白在原核期重组胚中消失，而在32-细胞期重组胚中再度出现，发生了蛋白合成的重序过程。

Finally, transfer 8-16 zygotes into oviduct of pseudopregnant female recipients mice.

最后，将注射后的受精卵植入代理孕母鼠输卵管中。

The vector was linearized and injected into 817 fertilized eggs of mice in which coagulation factor IX gene has been knocked out. The manipulated embryos were transferred into the oviducts of 45 pseudopregnant females, from which 63 offsprings were obtained.

将其线性化后，用显微注射法注射入817只凝血因子Ⅸ基因剔除小鼠受精卵雄原核，再将它们分别回输45只假孕受体母鼠的输卵管中，共产仔69只，存活63只。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

During the process of nuclear reprogramming, the zygotes of the normal embryos and the donor nuclei of thecloned embryos cease their unique repertoire of gene expression and reset their gene expression to the totipo-tent status, and then the normal and cloned embryos redifferentiate from the totipotent status to variousdifferentiated states for tissue generation or organogenesis.

通过细胞核重编程，首先，受精卵和克隆胚胎的供体核停止其特有的基因表达程序，恢复为全能状态的基因表达程序；然后，受精胚胎和克隆胚胎的细胞再从全能状态重新进入分化状态，最终形成各种组织和器官。

Objective To study the male infected by hepatitis B virus,whether the spermoblast takes the HBV or not,was passed by fertilized egg the filial generation,the formation father with baby's transmission.

目的 了解男性感染乙型肝炎病毒后，精液中是否携带HBV经受精卵传给子代形成父婴传播。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！