受精卵

To the oosperm with not stable bed of this kind of move, although pregnant woman does not drop,oosperm is worn the pregnant woman with stable bed, drop quite again sometimes and do not produce miscarriage.

对这类着床不稳定的受精卵，即使孕妇不跌跤也可能发生流产；而受精卵着床稳定的孕妇，有时跌得挺重而不发生流产。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法：将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体，应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核，再将注射后的受精卵移植至假孕鼠的输卵管。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

Now Lewis Wolpert has bet Rupert Sheldrake a case of fine port that:"By 1 May 2029, given the genome of a fertilised egg of an animal or plant, we will be able to predict in at least one case all the details of the organism that develops from it, including any abnormalities."

如今Lewis Wolpert跟Rupert Sheldrake打赌说："到2029年5月1日，只需一颗受精卵，无论动物还是植物，我们就能预测出至少在一种情况下这颗受精卵成长过程的全部细节，包括所有异常情况。"

The optimization of heat shock conditions for producing tetraploidy loach was treated the fertilized eggs for 4 min with 41℃ water after fertilization about 20 minutes.

采用热休克抑制受精卵第一次卵裂、Ag-NOR计数鉴定四倍体胚胎的方法，探索了诱发四倍体泥鳅的最佳条件是：在受精后20 min左右采用41℃热水持续处理泥鳅受精卵4 min。

Anti-endometria antibody IgG react with uterus internal membrane disturbing the embedding and growth of the fertilized egg. The tilter of the anti-endometria antibody IgG has relationship with the early habitual abortion.

抗子宫内膜抗体IgG可与子宫内膜发生抗原抗体反应，干扰受精卵的着床和发育，可导致反复流产，而且其影响受精卵的着床和发育的程度与血清中的抗子宫内膜抗体IgG的效价有关。

In these species, fertilized eggs develop into females and unfertilized eggs into males.

在这些种类的黄蜂身上，受精卵发展成为雌性黄蜂，未受精卵发展成为雄性黄蜂。

The zygote replicates itself, then we have many copies of zygote, they are called the stem cells.

受精卵自我复制，我们将会有很多分裂的受精卵，他们被称为干细胞。

Methods Using the means of culturing amphicytula in vitro to 2-cell and tubal insufflations to collect the 2-cell amphicytulas, then did embryo freezing, and compared the effect of superovulation of FCB mouse which live for 4~6weeks, 6~8weeks.

对4~6周、6~8周、8~10周FVB小鼠进行超数排卵效果的比较及采用体外培养受精卵至2~cell法和输卵管吹卵法两种不同方法收集2~cell受精卵进行胚胎冷冻。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。