受精卵

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

Some poeple remove them prior to incubation, but this should be done only if it's easy to seperate them from the rest of the egg mass.

一些朋友喜欢及早把未受精卵分离出来，只要能够轻松得分离的话，倒无所谓，最怕是破坏到相连的蛋壳。

In the spring of 2002, 11 different shell colors lines including 4 orange ,3 purple and 4 white were successfully established by using the mate strategy of self-fertilization.

利用壳色可遗传的特性，采用自体受精的交配策略，于2002年成功地建立了4个橙色、3个紫色、4个白色等3类共11个海湾扇贝自交系，并对不同家系的受精卵大小、胚胎孵化率及幼虫、稚贝和养成阶段的生长速率与存活率等生物学参数进行了比较。

Maybe this is your way of testing my love and devotion to you--I have always suspected that I might be too selfish and self-involved to be a good caregiver.

我的医生本来也告诉过我，除了黄体不足的流产可以挽救外，其他大多数的都是因为受精卵本身缺陷，没有办法的。

Self-incompatibility (self-sterility) The condition in whereby male gametes cannot fertilize female gametes from the same individual and give rise to a viable embryo.

自交不亲和性：是雄配子体不能与来自同一植物体的雌配子体发生受精作用并产生可育受精卵的情况。

It develops from the zygote and comprises the foot, seta, and capsule.

由受精卵发育而来，包括基足、柄和孢蒴。

The doscis decided by the maturity of the parent fish s sex gland.

受精卵在盐度27.9-33.5，水温26.5-30.5℃，pH8.2的水环境中经15-17h孵化。

The head of the sperm cell and the nucleus of the egg cell then unite and form a new cell: the zygote.

然后，精子的头和卵子的核融合，形成一个新细胞：受精卵。

This suggests that CaHIRA plays a role in the early embryo development and the late stages. The different expression characters of hira gene in these fish species may be related to the different reproduction models. The research on Drosophila has indicated hira gene related to sperm nucleus decondensation during fertilization. The high expression of hira gene before fertilization is prepared for the sperm nucleus decondensation and the formation of male prenucleus because colored crucian carp is bisexual reproduction. However, the sperm nucleus is not decondensated during the fertilization and is only to activate egg nucleus in gynogenetic gibel carp.

在彩鲫中，从未受精卵到胚胎发育的各个时期均可检测到表达，但表达量发生明显变化，受精后表达量迅速下降，直到原肠胚期表达量有所上升，并一直保持到出苗。hira基因在两亲缘种成熟卵子受精前后表达情况不同，可能与两种鱼不同的生殖方式有关。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。