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At fertilization one male nucleus fuses with the gee nucleus to form the zygote, while the second male nucleus fuses with the primary endosperm nucleus to form a triploid cell, or more rarely a second diploid or a pentaploid cell that later gives rise to the ENDOSPERM.

受精作用发生时,一个精核与卵核融合形成受精卵,第二个精核与最初胚乳核融合,形成三倍体细胞,偶而也形成第二个二倍体或五倍体细胞,三倍体细胞最后发育为胚乳。

PKC activation also caused failure of the second polar body emission after fertilization.

PKC活化也引起受精卵第二极体排出的失败。

Objective To investigate the relationship between the first polar body morphology of oocyte and the zygote morphology in pronucleus stage, embryo quality in cleavage stage.

目的 探讨卵细胞第一极体形态与原核期受精卵形态、卵裂期胚胎质量的关系。

Double fertilization The fusion of one pollen nucleus with the egg to form the zygote and of the other pollen nucleus with a polar nucleus to form the triploid endosperm nucleus.

双受精:指花粉粒中的一个精子与胚珠中的卵细胞结合形成受精卵,另一个精核与极核结合形成三倍体的胚乳核。

Cleavage polyembryony The splitting of an embryo into several identical parts, each of which may develop into a mature embryo. It is form of natural cloning.

裂生多胚现象:一个受精卵在发育过程中胚原细胞分裂为几个胚的现象,是自然克隆的一种形式。

The effects of salinity on the buoyancy hatching and abnormal rate of fertilized eggs of ovate pompano at 10 different salinities ranging from 5.0 to 50.0 were studied. Moreover, the survivorship as well as survival activity index of larvae of T.ovatus at 8 different salinities ranging from 10.0 to 45.0 were also observed.

观察比较了10种不同盐度(5.0~50.0)条件下卵形鲳鲹受精卵的沉浮性、孵化率和畸形率,以及8种不同盐度(10.0~45.0)下仔鱼存活情况及不投饵存活系数(survival activity index,SAI)。

AIM: To establish a transgenic mouse model of Hi1tchirtson-Gilford progeria syndrome and observe the effect of mutant lamin A on the implantation and survival ability of injected mouse zygotes.

目的:制备Hutchinson-Gilford早老症转基因小鼠模型,观察突变的核纤层蛋白A对小鼠受精卵着床和成活情况的影响。

To make the transgenic mice: We purified the gene fraction nestin-hsp68-EGFP and injected it into pronucleus of mouse zygotes to generate the transgenic mice.

1。转基因小鼠的获得:纯化基因片段nestin-hsp68-EGFP,用显微注射方法把基因片段注射到受精卵的雄原核中,获取转基因小鼠。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法,将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部,然后将胚胎移植到输卵管内,13只受体怀孕产仔。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵显微注射法,制备hbv转基因小鼠,用pcr,elisa,荧光定量pcr和免疫组化的方法研究hbv基因在转基因小鼠体内的整合、复制和表达情况。

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