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A study on spermatheca of the freshwater crab,Sinopotamon yangtsekiense was performed with histologic and cytochemical metheds.

纳精囊是溪蟹雌性生殖系统的重要组成部分,在精子储藏、受精及排卵过程中起着十分重要的作用。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明:(1)在中华蚱蜢的精子发生中,PNA受体是由初级精母细胞合成,然后转移到膜上,在次级精母细胞,由于量的积累其质膜出现了强阳性标记;从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化,这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关:受精囊内与精巢内的精子表面的标记是相同的,说明质膜糖蛋白的修饰作用在精巢内就已完成。

Objective:To compare the efficacy of 2 revised long protocols by triptorelin in in vitro fertilization and embryo transfer and intracytoplasmic spermatocyte injection.

目的:比较两种修正的达必佳长方案在体外受精-胚胎移植和卵母细胞单精子显微注射中的作用。

We think that centrin play an important role in meiosis during Urechis unicinctus sperm metamorphosis difference and we also think that centrin may be related to trochophore cilia and sperm tail formation,and reconstraction of centrosome after fertilization.

我们推测Centrin在单环刺螠精子发生过程中减数分裂或精子细胞变态分化过程中发挥作用;在单环刺螠早期胚胎发育中,Centrin可能与受精后中心体的重建及担轮幼虫的纤毛形成有关。

Two molecular forms of Xenopus hatching enzyme, 60kD and 40kD molecules, was obtained during preparation and purification. Both of them were verified as the hatching enzyme molecules, using anti GST UVS.2 antibody as the probe. 60kD molecule was digested or autodigested easily into 40kD molecule during purification. It was indicated that 40kD molecule was probably derived from 60kD molecule with its two CUB repeats lost, and the two CUB repeats may play an important role in recognizing and/or modifying of the molecular structure of vitelline envelope.

在分离纯化非洲爪蟾孵化酶时,得到了60kD和40kD两种分子,用孵化酶的特异性抗GST-UVS.2抗体进行Western杂交的结果证明二者均为孵化酶分子。60kD分子很不稳定,在纯化过程中极易降解,40kD分子可能是由60kD分子经过降解或自身降解丢失了两个CUB重复区而形成的,而CUB重复区很可能在对受精膜分子结构的修饰或改造中具有重要作用。

Data accumulated up to now have indicated the important role of MR in fertilizing modulation of human.

有关资料表明,MR在人类受精调节中可能起重要作用,但迄今对MR的表达、定位和功能等基本问题尚无较一致的看法,极待进一步深入探讨。

Methods Human luteinized granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and were cultured with M199 medium plus various concentration of leptin (0, 10, 30, 100, 300 ng/ml),human menopausal gonadotropin (hMG, 0, 0.1, 0.2, 0.5, 1, 2, 5, 10 IU/ml) and testosterone 100 μg/ml. For 2 days the media were collected for estradiol and progesterone measurements.

方法将来自体外受精-胚胎移植的卵巢黄素化颗粒细胞纯化后,在不同浓度瘦素(0、10、30、100、300 ng/ml)和人绝经期促性腺激素(hMG,0、0.1、0.2、0.5、1、2、5、10 IU/ml)单独或联合作用下进行体外培养,收集培养液,采用放射免疫方法测定颗粒细胞产生雌二醇及孕酮的量。

The structure of both synergids is clear, but the developmental mechanism and the roles during fertilization are not yet understood.

助细胞的形态结构已研究得比较清楚,但有关助细胞的发育机理和它在受精过程中的作用则仍不明确。

Superovulated C57BL/6J female mice were sacrificed at 0.5 dpc and injected purified BAC into male pronuclei.

将由超排卵作用受精后0.5天的C57B/6J母鼠牺牲,并注射纯化后的重组MLL基因至精核中。

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