受染

The most popular wool mordants in current use are alum and cream of tartar and they are quite safe.

当前使用的最受欢迎的羊毛媒染剂是明矾和酒石，他们是非常安全的。

But chemical mordant, such as alum are popular today.

但化学媒染剂，如明矾当今很受欢迎。

It's the most polluted street in Europe. Busses, beggars, blood.

它是欧洲受汚染最严重的街道，从公共汽车的汚染到乞丐与流血事件。

Milk fiber.The influence of pH value,dyeing temperature and neutral electrolytes on percent exhaustion were studied.The dyeing rates and buildingup properties of these dyes were also discussed.The effect of pH value was prominent on percent exhaustion,showing that the electrovalent bond between the dyes and the casein component of the fiber played an important role in the uptake of dyes.The ...

染液pH值、染色温度、元明粉等对上染率的影响，以及这两类染料在牛奶丝上的染色速度和提升性能的研究表明，牛奶丝染色受pH值影响很大，说明染料与酪素蛋白之间的离子键结合对染料上染起重要作用；元明粉在各pH值下对上染率的影响显示，牛奶丝的等电点约为4；酸性染料对牛奶丝的初染率高、初染速度快，但80℃恒温下的上染速度却慢于蚕丝和羊绒；牛奶丝染色对pH值的敏感性大于蚕丝和羊绒，且其酸性染料上的提升力较蚕丝和羊绒差。

Micronuclei in the bone marrow polychromatophilic erythrocytes in each dose group of mice were examined,leucocytes number and 30 day survival rate of mice following whole-body 5.0?Gy γ irradiation were also determined.

通过照射前后给予受试物，检测各剂量组受照小鼠的白细胞数、骨髓嗜多染红细胞微核数，观察照射后30d的平均存活天数。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

We observed that FuGene6 regent had the highest transfection efficiency and lowest toxicity to auditory neurons in the three groups. The transfection efficiency was about 3%-10%, variable from experiment to experiment. However, when we used recombinant adenovirus vector to transfer LacZ gene into auditory neurons,β-gal expression was detected in more than 70% of both neurons and gilas at 100M0I viral concentration..

通过对不同转染方法的选择和转染条件的优化，其中FuGene 6介导的质粒转染，其转染效率最高，可达3％-10％左右，但不同批次结果重复性较差，易受不同批次细胞来源动物的个体差异及培养条件等因素的影响。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

By the way, osmanthus is susceptible to oak root fungus and I have had at least six big ones died for the same cause. The problem with oak root fungus is you do not know when the osmanthus roots may touch and contract the fungus. Once it gets it, we have to cut off the infected area and move it to a container. My two pot-grown ones are the survivors of the fungus.

顺便一提，桂花易於感染oak root菌，我至少有六大盆死於此因，对於此菌的麻烦，就是你不会知道桂花的根何时接触这个菌种，一旦染上，就必须切除受感染地方，移到另一个容器，我两盆种的就是染菌后的幸存者。

The cell in acidic situation presented a degenerative chang-ment,such as an increased lipid drop and empty cavity in the cell plasma,a py-knotic nuclei,the dilatation of the endoplasmic reticulum and Golgi saccules,mi-tochondrial swelling the number of ribose decrease,and the ribose dissociated inthe plasma,a lot of swallowing cavity,the fibric material decreased accompany-ing with the acid being stronger.

随培养基pH逐渐下降，上述过程因受酸性阻抑而延缓，细胞增殖速度也受影响，处于酸性环境中的软骨细胞呈退行性变化，表现为细胞浆内脂质颗粒与小泡增多、细胞核皱缩、核仁浓染、内质网线粒体膨胀、核糖体数目减少，且与粗面内质网结合的少，游离在胞浆中的多。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。