受保护的

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Chapter Two illustrates the nature of the international guarantee.At first,the writer analyses the meaning of collateral nature and its theoretical basis.Thewriter points out that in order to reinforce the protection of the guarantor'sinterest,the guarantee is located as collateral guarantee in all countries'traditional legislation.In other words,the validity of the guarantee contract isdetermined by the underlying contract,i.e.if the underlying contract isestablished,the guarantee contract is established,too;if the underlying contract isvalid,and the guarantee contract is valid too.It is true,otherwise.But thedoctrine of autonomy of private law dominates the civil law and commercial lawin all the countries,so the parties are permitted to alter the collateral nature of theguarantee by an agreement under the related laws worldwide.so the collateralnature of the guarantee is not the compulsory nature of the law.This lays a solidfoundation for the emerging of the independent guarantee.The writer thenanalyses the meaning of independence and the cause of the booming of theindependent guarantee.In fact,the independent guarantee was originated fromthe change of the collateral nature by the parties contract.As a result,the validityof the contract of independent guarantee is not determined by the underlyingcontract as the collateral guarantee,But the validity of the independent guaranteecontract is independent from the underlying contract,the establishment and thevalidity of the contract of independent guarantee are not subjected to theunderlying contract.

第二章对国际信用担保的法律属性进行了论述，首先分析了从属性的含义及其理论基础，指出虽然各国传统立法为了加强对保证人利益的保护，都将信用担保定位于从属保证，即保证合同的效力取决于主合同的效力，主合同成立，保证合同也成立，主合同生效，保证合同也生效；主合同不成立，保证合同也不成立，主合同不生效，保证合同也不生效，但是由于私法自治的精神主导着各国的民商法领域，各国立法都允许当事人通过合同约定对信用担保的从属性进行改变，所以保证的从属性从来就不是信用担保制度的法律强制属性，这也为独立保证制度的出现奠定了制度前提；其次分析了独立性的含义以及独立保证兴起的原因，独立保证实际上就是当事人通过合同约定对保证从属性的改变，使独立保证合同的效力不再象从属保证合同的效力那样取决于主合同的效力，而是使独立保证合同的效力独立于主合同的效力，独立保证合同成立不因主合同的不成立而受影响，也不因主合同的无效而无效。

The coins in your nation's collective pocket will also determine your stature when it comes to diplomacy, since in addition to standard military alliances, you can propose trade alliances, make national gifts, and trade entire regions to your partners, as well as demand protectorate status, which, if accepted, will require the subject nation to forfeit half of its profits to you and preclude it from declaring war (unless the nation decides to rebel and declare war on you).

这套金银纪念币在您的国家的集体口袋也将决定你的地位，当涉及到外交，因为除了标准的军事联盟，可以提出贸易联盟，使国家的礼物，和整个区域的贸易到您的合作伙伴，以及需求的保护状况，如果接受，将要求受国家丧失一半的利润给您和妨碍它宣战（除非国家决定反叛和宣战你）。

In August 2007, C-SPAN unveiled a new "C-SPAN Video Library" webpage, which will eventually provide free access to all of its past programs including Congressional proceedings, hyperlinked to corresponding Congressional Record entries that are not otherwise subject to copyright.

2007年8月， C - SPAN的推出了一种新的& C - SPAN的视频库&的网页，最终将免费提供其所有的过去的项目，包括美国国会的程序，链接到相应的国会记录条目，没有其他受版权保护。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。