受体

The efficiency of the nuclear transfer was 63.6%(63/99).Forty-seven clone embryos were transferred into 5 recipient goats. After embryo transfer, two recipient goats have not been seen oestrous within 2 oestrum, and one of the two recipient goats was examined positive by gravid testing, which testified the clone embryos were viable in the uterus of recipient goats.

阳性细胞克隆中的2株(M9，M10)已经进行了首次细胞核移植，核移植成功率为63.6％(63／99)，将47个发育较好的克隆胚胎进行了胚胎移植，共用受体羊5只，2只受体羊2个情期未见返情，在没有返情的2只受体山羊中有1只妊娠检测阳性，说明转基因克隆胚胚胎移植后在受体山羊体内发育良好。

Intravoneous injection of 0.5mg/kgBW jingsongling cause the 60 minutesrelaxation of goat gastric motor.The effects of jingsongling on the gastric motor ofgoat is major of effects of α2 -adrenoceptor agonist,as well as that of α1 -adrenoceptor agonist,the effects of α1 -adrenoceptor agonist concentrate at thecardic and pylorus;the function of α2 -adrenoceptor exit widely at cardic,pylorus,rumen,reticulum,omasum and abomasum,the function at different part is not the same.The lowestdose of jingsonglinginhibiting the amplitude and frequency of the gastricmyoelectric is 0.01mg/kgBW;the influence of jingsonglingto the gastricmotility of goat is dose-dependent,and the amplitude regain after the frequency ofgastric myoelectriv wave.When the goat revived,the gastric motor function is notreach the level of the normal;the major reason ofjingsongling cause the goat gastricrelaxation is the amplitude renew slowly.The intravoneous injection ofjingsonglingfirst,after 5 minutes,intravoneous injection the antagonist,the effect of prazosin and idazoxan mixed is best,which can fast reversal of theinhibition of jingsongling on the gastric motor of goat,the effects of idazoxan isgood,and the prazosin block the effection of jingsongling is better than that ofxylazine,especially at cardia and pylorus.When intravoneous injection of0.5mg/kgBW idazoxan and/or prazosin only,the amplitude of gastric myoelectric ingoat increases at a certain degree,but the frequency is not changed.

静脉注射0.5mk/kgBW的静松灵引起山羊胃蠕动弛缓长达60分钟；静脉注射0.5mg/kgBW的静松灵对山羊胃蠕动机能的影响是以α2肾上腺素受体作用为主的，兼有α1肾上腺素受体的作用，而且静松灵的α1肾上腺素受体的作用主要集中在幽门和贲门；α2肾上腺素受体的作用广泛存在于贲门、瘤胃、网胃、瓣胃、皱胃和幽门中，不同部位间有功能上的差异；静注0.01mg/kgBW的静松灵是对山羊的贲门、瘤胃、网胃、瓣胃、皱胃和幽门的肌电波频率和振幅均产生影响的最低剂量；静注一定剂量的静松灵对山羊胃蠕动的抑制作用与剂量呈相关性；而且山羊胃肌电波振幅的恢复比肌电波频率的恢复慢；苏醒时山羊的胃蠕动机能尚未恢复到正常状态；静松灵引起山羊胃弛缓的主要原因是由于胃肌电波的振幅恢复慢；静注0.5mg/kgBW的静松灵，5分钟后静注0.5mg/kgBW的拮抗剂时，咪唑克生+哌唑嗪的作用最好，能迅速地逆转静松灵对山羊胃蠕动的抑制作用，咪唑克生也有很强的拮抗作用，而哌唑嗪对静松灵的拮抗效果好于对隆朋的拮抗；单独静注0.5mg/kgBW的咪唑克生和/或哌唑嗪时，山羊的瘤胃、网胃、瓣胃、皱胃、贲门和幽门的肌电波振幅有一定程度的增强，而肌电波频率未见变化。

Although the intact-receptor based biosensors of the maxillary palp of cockroach Periplaneta Americana are not yet practical analytical devices at present. Yet, sensitive and selective responses to biomolecules involving amino acids and protein, as well as some simple substance such as salts and sugars have been firstly obtained. It is already evident that receptor based biosensors are enormously superior to isolated receptor biosensors in terms of response time and recovery times, thus, the intact receptor biosensor worth of further investigation and development.

总而言之，本论文首次从电生理信号上对美洲蜚蠊下颚须的化学响应进行研究，初步探讨了基于美洲蜚蠊下颚须的整合受体生物传感器的特性；发现了美洲蜚蠊下颚须能够整合其密集的感觉毛阵列对化学刺激的响应；得到了蜚蠊对氨基酸和蛋白质等生物分子以及简单物质如盐和糖等的敏感而具有选择性的响应信号，并发现了糖和蛋白质的特殊响应信号；表明了美洲蜚蠊下颚须是一种新型整合受体生物传感器的敏感材料，进一步深入地研究信号波形及其整合原理、探寻糖和蛋白质的特殊受体细胞等，对这一类型整合受体传感器的研究发展是很有意义的。

The effects of FC were abolished by intrathecal administration of kynurenic acid (50 nmol) or 2-amino-5-phosphonovaleric acid (AP-5, 0.5 nmol), but not by 6, 7-dinitroquinoxaline-2, 3-dione (DNQX, 5 nmol), picrotoxin (5 nmol) or strychnine (1 nmol).

，高频刺激前0.5小时鞘内注射特异性NMDA受体拮抗剂AP-5（0.5 nmol）或非选择性谷氨酸受体拮抗剂犬尿酸（50 nmol），FC的效应被阻断；non-NMDA受体拮抗剂DNQX（5 nmol）不能阻断FC的效应；〓（5 nmol）和甘氨酸受体（1 nmol）拮抗剂对FC的效应也无明显影响。

Puzzlingly, such effect is transient and depression is usually reinstated after the first episode of recovery sleep, or even after short naps .

睡眠剥夺可能通过改变5-羟色胺(serotonin 5-HT)系统起作用，5-HT_(1A)受体作为突触前的自身受体在调节中缝核5-HT神经元的活性中发挥着关键作用，并且作为突触后受体介导5-HT对边缘系统、皮层、下丘脑及其它一些脑区部位的作用，使得5-HT_(1A)受体的调节对于精神疾病非常重要。

According to the prediction results of secondary structure of receptor β chain, the general theory about relationship between cytokine and its receptor at binding site and blocking results of polyclonal antiserum against D1 (9～114 residure of β chain), the new three peptides, which comprised residures of 45～75, 56～75 and 66～75, were designed and synthesized.

依据对受体β链二级结构的预测、受体与细胞因子结合部位的结构理论以及抗受体β链D1(9～114)片段多克隆抗血清有阻断GM-CSF促进DMSO诱导HL60细胞增殖活性的结果，设计和固相合成了受体β链第45～75，56～75和66～75三个人工合成多肽片段。

Under the direct current electric field, the expression of PDGFR were increased obviously and distributed asymmetricly on the membrane of VSMC. This receptor were mainly focused on the cathodal side of cellular membrane in electric field. The AT1R were also enhanced in the cells after exposure to the electric field, and the expression of AT2R had showed the changeless.

改进的电场干预装置作用下，血管平滑肌细胞膜血小板源生长因子受体表达增加，部分细胞内血小板源生长因子受体表达上调，呈不对称分布，在电场负极面较集中；细胞中血管紧张素Ⅱ1型受体表达亦增加，但无明显不对称分布现象；血管紧张素Ⅱ2型受体表达改变不显著。

In present study, we demonstrated that μ opioid receptor and D1 dopamine receptor could form heteromeric complexes in membranes preparation from HEK293T cells transiently cotransfected with both receptors and from mice striatum.

在本工作中，我们通过免疫共沉淀和BRET法证明，HEK293T细胞上瞬时表达的μ阿片受体和D1多巴胺受体能形成异源二聚体，并证实μ阿片受体和D1多巴胺受体异源二聚体存在于小鼠纹状体中。

Histomorphometric studies were done to measure the following indexes: the IOD values of pulmonary, skeletal muscular and pleural small vascular walls from staining of AT1 and AT2 receptors, the pulmonary vascular density, muscularization of the nonmuscular vessels, percent wall thickness and proliferation of pulmonary small vascular walls.

结果：先心病患儿肺小血管壁AT1、AT2受体染色的IOD值均高于对照组(P=0.000)；先心病患儿骨骼肌和胸膜小血管壁AT1、AT2受体染色的IOD值均高于肺小血管(P＜0.05)；先心病患儿肺小血管壁AT1受体染色的IOD值与肺血管密度、非肌性肺血管肌化程度、肺小血管壁厚百分比和肺小血管壁细胞的增殖度相关(r分别为-0.783、0.742、0.521、0.657)，AT2受体仅与肺血管密度和血管壁厚百分比相关(r分别为-0.487、0.516)。

However, opioid receptors are not expressed naturally in great abundance, they are relatively labile and frequently difficult to solubilize as monomers. The availability of their cDNAs makes them good candidates for overexpression in heterologous cells, which offers hope for the purification of membrane receptors for biochemical and structural studies.

在生物体内，阿片受体的含量很低，分离纯化过程中受体蛋白不稳定、易形成多聚体。cDNAs的克隆成功使阿片受体的异源高效表达成为可能，为研究阿片受体蛋白的生化特性和结构特征创造了条件。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。