取物

The highest one, pig dung's, is about three times more than the sheep dung's and cow dung's; the smelliest and deepest fermentation liquid is the pig dung's and followed by cow dung's and sheep dung's.

第2期刘德江等：猪粪、牛粪、羊粪沼气发酵比较试验Ⅱ纯羊粪便发酵试验组：取采集的新鲜羊粪便42g，同时加入20g驯化好的接种物，把料

Can improve the survival ratio of seeding. Culturing in hormone media can make meristem tip survive once time, and the result is better than two-time-cultivation.3、The meristem tip with two phyllomes(0.3-0.4mm in size)were found to suitable for virus-free seeding culture that were cut from aseptic seeding, which led to higher survival and virus-free ratio, up to 72.1%and 54.2%.

在培养基中添加椰汁、香蕉汁等有机添加物可以明显提高成苗率。3、采用二次剥离茎尖的方法，从无菌试管苗上切取带2个叶原基(大小0.3-0.4mm)茎尖分生组织培养，成苗率为72.1%，脱毒率为54.2%。

The result makes clear, in the affiliation of saturated NaCl the quantity is 5.5 ML, bulk mark joins a quantity to be 0.125 ML for the PAN of 0.1%, the Maerbi of SDS and Triton X-100 is when 0.38~1.39, forms system has better extractive effect to Ni2+-PAN complexing content.

结果表明，在饱和NaCl的加入量为5.5 mL，体积分数为0.1%的PAN加入量为0.125 mL，SDS和Triton X-100的摩尔比在0.38~1.39时，所形成的体系对Ni2+-PAN络合物具有较好的萃取效果。

During extracting vacuum still bottom and hydrocyclone underflow of H-coal process,coal liquid could be clearly divided into deashed liquidproducts of ash yield less than 0.05% and ash-rich residue with ash yield greater than 60%.

在萃取氢-煤法减压蒸馏釜液或水力旋风分离器底流物时，这些重质物料可分离成灰分在0.05%以下的低灰液体产品和灰分达60%以上的富灰残渣。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Three tar mat s of Well Wenjian3 were discovered and determined accurately in Wenliu Oilfield in Dongpu Depression by thin layer chromatogram-flame ionization detector, whole hydrocarbon gas-chromatography, and the characteristics of reservoir sedimentology and logging response.

根据文留油田文检3井密闭取心层段岩心样品抽提物薄层色谱分析和全烃气相色谱分析、以及储层沉积学和测井响应特征发现并准确鉴定出3个沥青垫。

LiDAR ; Building Detection ; Structure Line Extraction ; Wavelet ; External Contours ; Internal Structure Lines

指导教授徐百辉；中文关键字光达；建物侦测；结构线萃取；小波；外部轮廓线；内部结构线

Solanesol was extracted in the waste tobacco leaves by dynamic saponification method of ethanol and solvent naphtha.

建立了以废次烟叶粗提物为原料，采用乙醇和溶剂油动态皂化方法萃取茄尼醇的工艺。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

Robert Williams was the first man ever killed by a robot. On January 25, 1979,Williams climbed into a storage rack at the Ford Motor's Flat Rock casting plant to retrieve a part because the parts-retrieval robot malfunctioned.

被机器人杀死： Robert Williams是第一个被机器人杀死的人，1979年1月25日，在福特汽车公司平顶石汽车制造厂，由于负责拿取零件的机器人出了故障，所以威廉姆斯爬上一个储物架拿一个配件。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。