取出

The principle is as follows: after incubating the standard sample of bacterial endotoxins, glycogen anti coagulation whole blood extracting from the healthy domestic rabbit, extralin anti congealable whole blood from healthy volunteers, THP 1 cells together, measure the releasing amount of TNF and IL 6 from cell gene by the method of ELISA as a endogenesis index judging the pro heat role of endotoxin.

其原理是：分别将细菌内毒素标准品与从健康家兔取出的肝素抗凝全血、健康自愿者提供的人肝素抗凝全血以及THP 1细胞共同孵育后，用ELISA双抗夹心法测定其产生的细胞因子TNF α和IL 6的释放量，作为内源性检测指标来判定内毒素的致热作用。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Objective to evaluate the functional recovery of acute spinal cord injuried rats treated with exogenous wnt-3a signal protein administration and to explore its mechanism.methods moderate spinal cord contusion injury was made in 40 adult sprague dawley rats at t10.twenty rats served as contusion controls(group 1).twenty rats were treated with wnt-3a for three days after injury (group 2).the functional recovery of the rats was observed through basso,beattie,bresnahan open field locomotor score.rats were killed at 14 or 28 days after injury,then spinal cords were removed for histopathological examinations,and the expression of the bromodeoxyuridine plus neural cell markers was stained with immunohistochemical method.results rats of two groups receiving a contusive injury recovered substantial function within 1 week.by 28 days,rats in groups 2 scored 7.0 points better on the bbb scores than rats in group 1 group 2=16.94,after 28 days vs.

目的 研究外源性wnt-3a信号蛋白对脊髓损伤的修复作用，并探讨其作用机制。方法取40只成年雌性sd大鼠在t10节段制备适度脊髓挫伤模型。随机从中取20只为损伤对照组(group 1)，另外20只为损伤治疗组(group 2)。脊髓损伤3天后用wnt-3a蛋白治疗。这些大鼠的功能恢复通过basso、beattie、bresnahan开放视野运动评分来观察。这些大鼠分批在损伤后14天或28天被处死，取出损伤节段脊髓用来组织病理学检查，同时用5-溴脱氧尿嘧啶核苷和神经细胞标记物进行免疫组化染色。结果两组大鼠在伤后一周运动功能有明显的恢复。不过，到损伤后28天，我们观察发现，损伤治疗组中的大鼠bbb评分比损伤对照组中的评分高出7.0分左右(group 2∶16.94±1.18，group 1∶9.89±1.29；p.05)，光镜和电镜检查发现wnt-3a蛋白对髓鞘形成和轴突再生有一定的修复效果。

Two patients with acromioclavicular joint dislocation recurrence were excluded, and coracoclavicular ligament therapy was not performed.

取出内固定后肩锁关节脱位复发2例，均未行喙锁韧带修复。

Methods From March 2002 to January 2006, 41 patients with Tossy type Ⅲ aeramiaclavicular dislocation were treated with AO/ASIF clavicular honk plates. The coracoclavicular ligaments were sutured in 15 eases, and the plates were removed 12 months postoperatively in 18 patients.

自2002年3月～2006年1月使用AO/ASIF锁骨钩钢板治疗41例Tossy Ⅲ型肩锁关节脱位患者，其中15例术中修补了喙锁韧带，18例在平均术后1年取出钢板。

The oldest sediments cored to date are about 150 million years old .

到目前为止取出的最老沉积物的岩心是大约1.5亿年以前的生成。

After heart perfusion, the spinal cord and brainstem were removed, and post-fixed overnight, then coronally sliced into 25μm thick of each piece.

取出后过量戊巴比妥钠麻醉，心脏灌流，取脊髓和脑干，后固定过夜，冰冻切片，片厚25μm。

The right parietal cortex contusion was made with stab wound and the rat was killed at various survival time( 30min, Ih, 3h, 6h, 12h,ld, 3d, 7d, 14d, 21d). Paraffin sections(4 μ m) were cut coronally of the whole brain and stained with antibody of GFAP, the hemotoxylin eosin stain was done in the mean time.

实验组用针刺法造成大鼠右顶叶脑挫伤后，按30min，1h，3h，6h，12h，1d，3d，7d，14d，21d不同时间分批处死动物并立即取出全脑，同时颈静脉取血2ml，正常对照组不造成颅脑损伤，直接处死动物后立即取材。

The VFA in the ruminal fluid can be extracted completely by mixing it with chloroform containing an acid adsorbent and also crotonic acid as an internal standard.

瘤胃液仅在存有一种酸性吸附剂的氯仿溶液中，即可萃取出所含全部VFA。

Take out of the locking intramedullary pin from the patient with femur＇ s disunion,and debride thoroughly,then transplant the fibula of the patient himself to the femur＇ s medullary cavity and transplant the ilium to the location of the disunion.

对15例股骨骨折带锁髓内针内固定术后骨不连患者行取出髓内针，彻底清创，自体腓骨髓内移植，骨不连处植髂骨，全身抗感染和局部持续灌注冲洗引流。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。