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METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.

无菌条件下取新生小鼠脑组织,制备单细胞悬液。

METHODS: Rat cerebral cortex was obtained sterilely, made into monoplast suspension by the mechanical separation, and then incubated in the serum-free MEM/F12 medium, supplemented with B27 neurotrophic additive for 1 week. Subsequently, cells were passaged.

无菌条件下取胎鼠大脑皮质,剪碎后机械分离法制作单细胞悬液,加入含B27神经营养添加剂的无血清MEM/F12培养基进行培养,1周后传代。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组,以体外直肠不全结扎法造模,以动态浊度法检测各组大鼠血浆内毒素水平,放射免疫分析法检测各组血清TNF-α含量;②大鼠处死后,取左肺进行支气管肺泡灌洗,以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平;③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数,计数兰染的细胞数,求出死亡百分率;在光镜下计数BALF沉渣200个细胞,计算中性粒细胞所占百分比。

After the experiment the nephridial tissues were made into histological sections, the pathological changes were observed through microscope.

实验结束后,取各组兔肾脏制成切片,HE染色后镜下观察肾组织病理学变化。

Objective To evaluate the clinical safety efficiency of percutaneous nephrolithotomy in patients with complicated upper ureter calculi.

目的 探讨经皮肾镜下应用第三代弹道超声碎石清石系统治疗复杂性输尿管上段结石的临床效果及操作体会方法总体分析海口市人民医院应用经皮肾镜取石术治疗91例患者的临床资料。

More experimental data of condensation heat transfer coefficient, especially ones obtained at some determined pressure of mixtures and concentration of noncondensable gases, are required in order to make a data bank. Therefore, the experiment is arranged for steam-air condensing on the outside of a horizontal smooth tube, and 405 data have been obtained for the pressure of the bulk mixture covering 0.1~0.5bar and the mass fraction of air 0.3%~10.0%.

得到了一些重要结论,尤其是首次得到了&同样含量的空气对压力较高的混合气体冷凝换热的削弱作用较小&、&蒸汽侧冷凝换热系数近似与空气质量成分的对数成正比关系&、&空气含量的增加对蒸汽侧冷凝换热系数的影响随壁面过冷度增大而减小&及&管内冷却水流速取值范围的上、下限随管外蒸汽侧空气含量的增大而减小&等结论,并验证了在本文实验范围内&蒸汽侧冷凝换热系数与相同温度下纯净饱和蒸汽凝结换热系数的Nusselt理论预测结果的比率随壁面过冷度增大而减小&的数值分析结论〓。

We look forward to the digital business card printing and membership card making the arrival of the new millennium, ink-jet technology because they will have no version, nonpressure, digital files, system stability, substrate features: wide, with its own technology to improve, in the digital environment, is showing very strong momentum of development.

我们等待着数字化制卡和会员卡制作新纪元的到来,喷不朱技艺由于其无版、无不抬、拔取数字文件、编制处事安定、承印物普及等特点,陪同其自身技艺的不停美满,在数字化不小境况下,侧表现不入极其强劲的成长势脚。

All rats were fed under the same conditions for 7 days and sacrificed. Bilateral orchidectomy was performed and germ cell apoptosis was detected.

按Turner法建立睾丸扭转/复位模型,所有大鼠均在同等条件下喂养至术后7 d处死,切取双侧睾丸后检测凋亡指数。

My "orna" just dropped out, would you mind to hold on to it I am coming down.

我的&布&刚掉下,我下来取你不介意吧?

"A trucut needle biopsy device that can be used to obtain specimens from the pancreas and other perigastric organs under EUS guidance has been developed and successfully tested in animals," write Alberto Larghi, MD, PhD, from the College of Physicians and Surgeons, Columbia University, in New York City, and colleagues.

来自于纽约市美国综合大学内科医生和外科医生学院的Alberto Larghi, MD,PhD和其同事表示,能够在EUS原则下取得胰腺和其它的在胃周围附近器官的组织样品的一个trucut引导针取活组织检查装置已经被发展并在动物中成功地测验。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。