取下

Mscs were cultured in a conditional medium in subculture including: dexamethasone, vitamin c, and β-glycerophosphate.

sd大鼠12只，在全麻及无菌条件下取其左侧股骨的骨髓基质细胞，并用矿化液诱导培养。

The neck of femur were cut and stined with Masson Golder Trichrome for bone histomorphomemetric contrast group analysis.

在给药后3、4.5、6个月每组各取10只大鼠进行检测，先测量体重，空气栓塞处死，分离股骨颈，获得股骨颈样本，经切片等处理后，显微镜下作骨形态计量学检测。

Methods］ninety femal sd rats,weight 230～280 gram,were rangdonmized into three groups: qiangguyin group,miacalcic group and control group.all rats were variectomized.after 10 weeks,the osteoporosis model succeed,the treatment group,contrast group were given qiangguyin,miacalcic respectively.the treatment group used qiangguyin everyday,1ml/g.the contrast group used miacalcic 0.72u/kg,everyday hyodermic.and 3,4.5,6 months after the treatment,kill 10 rats to explore.the neck of femur were cut and stined with masson golder trichrome for bone histomorphomemetric contrast group analysis.

方法］健康雌性sd大鼠90只，体重230～280g。随机分为3组，分别为治疗组、对照组和空白组，每组30只。分别采取切除双侧卵巢方法进行骨质疏松造模，10周造模成功后，开始给药，治疗组用自拟强骨饮灌胃，每日一次，每次1ml/g，对照组用密盖息，皮下注射，每日1次，每次0.72u/kg。空白组，不做处理。在给药后3、4.5、6个月每组各取10只大鼠进行检测，先测量体重，空气栓塞处死，分离股骨颈，获得股骨颈样本，经切片等处理后，显微镜下作骨形态计量学检测。

The steps are as the following:1、Preparing the cerebrospinal fluid one hour before the experiment,take some of them for cryopreservation;2、Without paralyse,cuting down the rat head quickly and dislodge the hippocamp part which is used in the experment in freezing liquid,and trim the hippocamp distrct.3、Fixing the hippocamp tissue on the vibratome,to cut down a brain slice with 300μm,use the haustorial tube to move the slice to the preincubate dish,cultivanting it for one hour.Then move it to the record incubation chamfer.4、Preparing the glass micloelectrode and fill it with NaCl,the corcentation is 3mol/L.5、adjusting the recorder,after cultivate the brain slice in the incubation chamfer for 2 hours,the expermentize can be gone on.6、In order to ensure the accurate of the experimental result,use only one medicine concentration for one brain slice in every experiment.

本实验研究方法是：1、实验前1小时配制好所需脑脊液并充以95% O2和5% CO2的混合气，取小部分冷冻保存；2、在乙醚麻醉下，快速断头并在冷冻液里取出包含海马的大脑部位，修整出所需海马区；3、将海马组织固定于振动切片机上切出300μm厚的海马脑片，用广口吸管转移至预孵育皿培养一小时，后再转移到记录用孵育槽内；4、拉制好玻璃微电极，并充以3mol/L的NaCl；5、调试好记录仪器，将脑片在孵育槽内培养2小时后进行实验；6、为了保证实验结果的准确性，每一块脑薄片只进行一个浓度的药品实验。

College of Veterinary Medicine, China Agricultural University, Beijing 100094, China Abstract: A method has been developed to determine residual stilbenes such as diethylstilbestrol, dienestrol and hexestrol in animal tissues using solid phase extraction and gas chromatography-mass spectrometry.

动物组织样品在碱性条件下用乙酸乙酯提取，经碳酸钠溶液液-液分配净化，再用硅胶柱固相萃取净化，洗脱液均分成两份后经氮气吹干，分别用双三甲基硅基三氟乙酰氨和七氟丁酸酐衍生，采用选择离子监测模式进行测定，外标法定量。

MATERIALS AND METHODS: Bilateral hippocampi were isolated from neonatal Wistar rats; cellular suspensions were prepared under sterile conditions.

材料与方法：取新生Wistar 大鼠双侧海马，无菌条件下制备细胞悬液。

Histomorphology changes of liver tissue were observed with light microscope.

再灌120min后，各组取血及肝组织，测定血清及肝组织中SOD活力、MDA和NO水平，光镜下观察肝组织形态学改变。

PRETTY RICKY LYRICS "Your Body" Yes sir Yes sir Yes sir Yes sir I got new shoes on the ride Rollin' down 95 And you can see in my eyes That I'm lookin for a cutiepie And we ain't gotta make love And we can just cuddle up But if she want me to beat it up Then dammit, I'll beat it up I don't know why, but the ladies call ol' baby blue the sticker They take me and rape me and make me they victim I lick em and freak 'em if they married I see ya If they look like wifey material, then I keep 'em Stuntin' through the city tryin' to find a lady who Beautiful, but she gotta have booty too Baby blue gonna let you do what you wanna do You can feel on it if you really want to Get a taste of the salami knock knock knock knock you down like a tsunami bust in you like atomi-ee I'mma ahead of my class gettin' head in the jag look in the duffle bag see benjamin heads on the cash I got new shoes on the ride Rollin' down 95 And you can see in my eyes That I'm lookin for a cutiepie And we ain't gotta make love And we can just cuddle up But if she want me to beat it up Then dammit, I'll beat it up Top down blue star tag Ol' master bear skin rugs in the jag Spectac with the bad chick in the back tryin ta beat it up like an Everlast punching bag hotter than a bisquick biscuit out the oven your baby mama go on missions to get this lovin we kissin and huggin she never pick her phone up You be lookin for her while we doin the grown up she complain when she catch back spasms, but she love when she get the back to back orgasms yes sir, the game is automatic, give it to 'em one time They come back like addicts.

漂亮祥歌词&你的身体&不错不错先生先生不错不错先生先生我有新鞋就骑滚动'下来95 ，你可以看到我的眼睛，我lookin 一cutiepie ，我们并不推荐做爱，大家只能偎，但如果她要我打起来，然后dammit ，我会打起来：我不知道为什么，但是各位请职的婴儿蓝的贴纸，他们带我和强奸我，使我对他们的受害者我舔EM和怪物'电磁如果他们结婚，我看亚若看起来好像wifey材料，钍恩，我保持'电磁stuntin '通过市tryin '找一位美丽，卜笔，她所推荐的战利品太婴儿蓝gonna让你做你想做的事，你可以感受到它如果你真的想要有品味的萨拉米敲叩敲你敲下来像李元簇纳米胸像你像原子- 41 i'mma提前班上极少'头军法看在达夫尔袋本杰明见首长的现金我有新鞋就骑滚动'下来95 ，你可以看到我的眼睛，我lookin一个cutiepie ，我们爱 1982推荐做爱，大家只能偎，但如果她想我会在它上升 dammit话，我会它击败了由上而下蓝星标签醇'师父紧皮肤地毯在军法萃取受检者与坏鸡的背部tryin电讯拍起来像一个everlast出气筒炎热比之二快速饼干出烤箱婴儿你妈妈去出差要想让这个lovin我们基辛和1001-1560她氮以往接她的电话了，你能lookin她虽然我们doin的长大，她抱怨，当她三曲线回痉挛，但她的爱，当她得到背靠背高潮不错先生， 77 饰物是自动的，它给予『电磁一次他们回来一样上瘾。

The invention relates to a synthesis method of N-acetylaminoglucose, which comprises dissolving D-glucosamine hydrochlorate and alcohol at the mass ratio of 2-2.5:100, adjusting the pH value of the solution to 9-10, filtering, taking the filter liquor, mixing and adding acetic anhydride at the 50-60wt% amount of D-glucosamine hydrochlorate, mixing and reacting for 2h, using absolute ethyl alcohol to recrystallizing, filtering and recrystallizing via water, to obtain rod-shape food grade N-acetylaminoglucose.

本发明涉及一种N-乙酰氨基葡萄糖的合成方法。该方法的具体步骤为：将D-葡萄糖胺盐酸盐和乙醇按2～2.5∶100的质量比溶于水中，调节该溶液的pH值为9～10；过滤，取滤液，搅拌下加入乙酸酐，其加入量为原料D-葡萄糖胺盐酸盐量的50～60wt％；搅拌反应2小时后，用无水乙醇进行重结晶；过滤，晶体再用水重结晶，得棒状食品级N-乙酰氨基葡萄糖。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。